2018
DOI: 10.1590/0074-02760170250
|View full text |Cite
|
Sign up to set email alerts
|

Leishmania (Viannia) braziliensis isolated from the saliva of patients in a cutaneous leishmaniasis-endemic area of northeastern Brazil

Abstract: Several studies have described the use of non-invasive collection methods, mostly based on the detection of parasite DNA, for diagnosis. However, no Leishmania specimens have been isolated from saliva. Here, we report the first isolation of Leishmania braziliensis from the saliva of humans with cutaneous leishmaniasis but without lesions on their mucosa. The isolates were obtained from salivary fluid inoculated in hamsters and were tested by multilocus enzyme electrophoresis. Seven samples from 43 patients sus… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1

Citation Types

0
3
0

Year Published

2019
2019
2024
2024

Publication Types

Select...
5

Relationship

1
4

Authors

Journals

citations
Cited by 5 publications
(3 citation statements)
references
References 18 publications
0
3
0
Order By: Relevance
“…It was demonstrated that PCR is better to define the patient diagnosis of TL in all types of the analyzed biological samples. Therefore, the combination of minimally invasive sample collection procedures (such as the swab of lesion, salivary fluid, and blood) with molecular PCR test for diagnostic confirmation of TL is very important 25,[29][30][31] . Especially when compared with classic parasitological tests with lower positivity 10,26,32 and need for invasive collection (lesion puncture and biopsy).…”
Section: Discussionmentioning
confidence: 99%
“…It was demonstrated that PCR is better to define the patient diagnosis of TL in all types of the analyzed biological samples. Therefore, the combination of minimally invasive sample collection procedures (such as the swab of lesion, salivary fluid, and blood) with molecular PCR test for diagnostic confirmation of TL is very important 25,[29][30][31] . Especially when compared with classic parasitological tests with lower positivity 10,26,32 and need for invasive collection (lesion puncture and biopsy).…”
Section: Discussionmentioning
confidence: 99%
“…Cultures are performed with fine needle aspirations or cutaneous biopsies. However one study, which has been performed on 7 CL patients with lesions in extremities and no mucosal lesions, reports the isolation of the parasite in saliva, which has promising implications on disease surveillance if further tested on a larger cohort [27]. In Sri Lanka, polymerase chain reaction (PCR) is usually performed on biopsy samples.…”
Section: Sample Collection Methodsmentioning
confidence: 99%
“…The presence of viable Leishmania parasites in the saliva of infected patients was demonstrated in 1934 by Forkner [221]. More recently, L. braziliensis was recovered from the saliva of a person suffering from cutaneous leishmaniasis [222]. A large number of studies describe the successful detection and identification of Leishmania DNA in saliva or oral swabs, with PCR or other methodologies of DNA amplification (LAMP).…”
Section: Saliva/oral Swab/sputummentioning
confidence: 99%