Leishmania Species in Two Regions of Paraná, Brazil: Biochemical Characterization by Isoenzyme Electrophoresis

Soccol, Vanete Thomaz; Castro, Edilene Alcântara de; Luz, Edna Dora Martins Newman; Dereure, J.; Pratlong, Francine; Membrive, Norberto; Dedet, Jean-Pierre

doi:10.1007/978-94-017-0203-4_35

Cited by 4 publications

(6 citation statements)

References 21 publications

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“…With the specific primers, we verified that the two patients from Argentina and one from Mato Grosso were carriers of L. (L.) amazonensis, and 70 from Paraná, São Paulo, Mato Grosso and Santa Catarina of L. (V.) braziliensis. Therefore, based on our PCR results, it was possible to demonstrate that L. (V.) braziliensis is autochthonous in the Paraná State and is the species responsible for CL and MCL in humans from this state as demonstrated by Thomaz-Soccol et al(26). The prognosis of human leishmaniosis depends in large part on the parasite species and also on the diagnosis time.…”

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confidence: 62%

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Molecular diagnosis of leishmaniosis in the Paraná state of southern Brazil

Pereira

Thomaz-Soccol

Lima

et al. 2008

Experimental Dermatology

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The objective of the present study was to establish a polymerase chain reaction (PCR) technique for the diagnosis of cutaneous and mucocutaneous leishmaniosis from autochthonous cases in the state of Paraná in southern Brazil as well as imported cases. We sought to determine its utility and accuracy compared with smears and present culture methods. To standardize PCR samples, skin and mucosal punch biopsies from human lesions were performed on patients living in different regions of the Paraná state (76 cases) and other endemic areas of Brazil and Argentina (7 cases). For PCR standardization, two pairs of primers (MP1L/MP3H and B1/B2) were utilized for amplification of the conserved sequences in the minicircle of kinetoplast DNA (kDNA) for the Leishmania braziliensis complex. Two other primer pairs (b1/b2 and a1/a2) were species-specific for L. (V.) braziliensis and L. (V.) amazonensis, respectively. After differential diagnosis, eight patients had clinical diagnosis of the cutaneous ulcer changed to others pathologies such as syphilis, baso-cellular carcinoma, varicose ulcer, ecthyma and paracoccidioidomycosis. Of the 75 patients with cutaneous (CL) and mucocutaneous (MCL) lesions who provided samples, 47 (46 CL + 1 MCL) were diagnosed with leishmaniosis by smear and 57 (52 LC + 5 MCL) were diagnosed by culture methods. In contrast, our PCR technique presented higher accuracy when compared to the direct examination and culture of parasites. PCR is applicable both for CL where all 61 lesions were diagnosed, and MCL where 12 of 14 lesions were diagnosed. This molecular biology technique is also a faster and more specific diagnostic method compared with present parasitological procedures.

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confidence: 62%

“…Medeiros et al (24), using the primers 13A and 13B, obtained 81.5% (26). Medeiros et al (24), using the primers 13A and 13B, obtained 81.5% (26).…”

Section: Discussionmentioning

confidence: 97%

Molecular diagnosis of leishmaniosis in the Paraná state of southern Brazil

Pereira

Thomaz-Soccol

Lima

et al. 2008

Experimental Dermatology

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“…The antigenic diversity due to molecular and biochemical differences of each complex or species has been characterized recently with modern molecular biology tools [4][5][6]. Some questions that remain are: Is the sensitivity the same when we use a homologous or a heterologous antigen for CL diagnosis?…”

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confidence: 99%

Comparison of serological and parasitological methods for cutaneous leishmaniasis diagnosis in the state of Paraná, Brazil

Szargiki¹,

Castro²,

Luz³

et al. 2009

Braz J Infect Dis

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“…In the State of Paraná, in southern Brazil, indigenous CL transmission has been detected in three regions: the Ribeira de Iguape River Valley (east), where the disease has been reported for more than a century (5) ; in northern Brazil, where an outbreak was reported in 1994 (6)(7)(8) and in the central region, where reports have been reported since 2002 (9) . In the same State, the parasite isolated from humans and dogs in the different regions was Leishmania (Viannia) braziliensis Vianna, 1911 (8)(9)(10)(11) . The vector Lutzomyia (Nyssomyia) whitmani (Antunes & Coutinho, 1939) was found infected with the same species in the northern part of the State (7) .…”

Section: Introductionmentioning

confidence: 99%