Comparison of serological and parasitological methods for cutaneous leishmaniasis diagnosis in the state of Paraná, Brazil

Szargiki, Regiane; Castro, Edilene Alcântara de; Luz, Edna Dora Martins Newman; Kowalthuk, Wolodymir; Machado, Ângela Aparecida; Soccol, Vanete Thomaz

doi:10.1590/s1413-86702009000100011

Cited by 40 publications

(52 citation statements)

References 25 publications

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“…19 Moreover, Szargiki et al, using parasitological methods, detected 79.3% of the patients with clinical diagnosis, and demonstrated a 95% sensitivity with serological methods for homologous antigens. 20 However, when IFA and MST positiveness rates were analyzed for diseased patients, rates reached 83.9% and 91.6%, respectively.…”

Section: Discussionmentioning

confidence: 97%

Epidemiological and clinical characteristics of cutaneous leishmaniasis and their relationship with the laboratory data, south of Brazil

Curti¹,

Silveira²,

Arraes³

et al. 2011

Braz J Infect Dis

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Objective: To evaluate clinical, epidemiological and laboratorial aspects for the understanding of the disease characteristics and its relationship with diagnostic tests. Methods: A retrospective, descriptive and analytical study involving 2,660 American cutaneous leishmaniasis (ACL) suspected patients from southern Brazil was undertaken between April 1986 and December 2005. Data on population characteristics and laboratory tests were obtained. Diagnostic laboratory tests used were direct search for Leishmania spp. (DS), Montenegro skin test (MST) and indirect immunofl uorescent assay (IFA). Results: 62.3% of patients were positive for at least one laboratory test. DS test was positive in 65.1%; MST in 92.3% and IFA in 70.0%. Although Cohen's Kappa test did not reveal any agreement with laboratory diagnosis for ACL, the association between MST and IFA tests increased positiveness to 98.9%; MST and DS to 97.2%; and IFA and DS to 85%. IFA and MST positiveness were higher among patients with ≤ 2-month lesion-evolution time. Most ACL patients were male (72.6%) in the age range of 15-49 years, featuring lesions during two months or less (53.9%) and a cutaneous form of the disease (88.0%). Conclusion: Findings suggest that for the adequate identifi cation of ACL cases a combination of laboratory tests that involves the association of MST with at least another test should be used.

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Section: Discussionmentioning

confidence: 97%

Epidemiological and clinical characteristics of cutaneous leishmaniasis and their relationship with the laboratory data, south of Brazil

Curti¹,

Silveira²,

Arraes³

et al. 2011

Braz J Infect Dis

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“…Thus, IIF-Leishmania was performed to compare the positivity of the ELISA-positive samples. The results of IIF were not in agreement with those obtained by ELISA, which may be a consequence of the higher sensitivity of the latter (Szargiki et al, 2009). Some studies have shown that IIF-negative samples could test positive for ACL in ELISA (Ferreira et al, 2006), including the subclinical cases (Arraes et al, 2008).…”

Section: Resultsmentioning

confidence: 71%

“…Several northwestern cities in the state of Paraná have shown a high incidence of ACL; therefore, it is an endemic region for this disease (Curti et al, 2009;Monteiro et al, 2009). Leishmania braziliensis is the main etiological agent of ACL in the state of Paraná (Lonardoni et al, 2006;Szargiki et al, 2009). The disease spectrum includes single, localized, cutaneous ulcers, diffuse cutaneous leishmaniasis and mucosal disease.…”

Section: Introductionmentioning

confidence: 99%

“…Enzyme-Linked Immunosorbent Assay (ELISA)-based methods of diagnosis are known to be highly sensitive (Yoneyama et al, 2007) and have been employed in the diagnosis of both clinical and subclinical cases of ACL (Arraes et al, 2008;Szargiki et al, 2009). Previous reports have also confirmed the applicability of Polymerase Chain Reaction (PCR) in the diagnosis of ACL, although it is not routinely used for this purpose (Yoneyama et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Serological and Molecular Investigation of Cutaneous Leishmaniasis in Healthy Individuals from an American Cutaneous Leishmaniasis-Endemic Region

Ribas-Silva

Navasconi

Braga

et al. 2015

American Journal of Infectious Diseases

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American Cutaneous Leishmaniasis (ACL) is a major, clinically relevant zoonosis in Brazil. The disease spectrum includes single, localized, cutaneous ulcers, diffuse cutaneous leishmaniasis and mucosal disease. A subclinical form of the disease has also been described in individuals living in ACL-endemic regions. The goal of this study was to employ immunological and molecular diagnostic methods to evaluate the presence of subclinical ACL in healthy individuals from an ACL-endemic region in northwestern Paraná.

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“…Additionally, ELISA is a relatively easy test to perform with fast 24 Laboratory diagnosis and clinical signs of canine visceral leishmaniasis in dogs examined at the Center for Zoonosis Control in Campo Grande -MS, Brazil results and a low cost (Alves and Bevilacqua, 2004). However, there has been disagreement in the literature regarding the need for two or more diagnostic methods to increase rate of correct diagnosis of leishmaniasis (Szargiki et al, 2009). When compared to clinical symptoms, the PCR-based diagnostic test showed the greatest correlation, with 45% to 55% agreement with each clinical symptom, followed by ELISA, with 34.5% to 54.5% agreement.…”

Section: Diagnostic Test Results Compared To Clinical Manifestationsmentioning

confidence: 99%

Laboratory Diagnosis and Clinical Signs of Canine Visceral Leishmaniasis in Dogs Examined at the Center for Zoonosis Control in Campo Grande – Ms, Brazil

Cunha¹,

Andreotti²,

Silva³

et al. 2012

AVS

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Visceral leishmaniasis is a type of zoonosis caused by several Leishmania species endemic to tropical, subtropical, and Mediterranean climate regions. Dogs are the primary source of infection in urban areas and can be symptomatic or asymptomatic. This study focused on the observation of clinical signs of leishmaniasis in dogs in Campo Grande, Mato Grosso do Sul, Brazil. Samples from affected animals were analyzed using indirect fluorescent antibody (IFA) tests, an enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR) assays to determine the optimal diagnostic tool for use on animals that present clinical symptoms. A predominance of clinical symptoms affecting the integumentary system was observed, and splenomegaly and hepatomegaly were the most important pathological signs. Among the diagnostic tests, the greatest agreement was seen between ELISA and IFA, followed by ELISA and PCR, and finally IFA and PCR. PCR diagnostic results showed the greatest extent of correlation with clinical signs, followed by ELISA and then IFA. When choosing a diagnostic method, veterinarians should consider the clinical signs and health status of the patient. Key Words: ELISA; PCR; IFA; RV1/RV2 primers, Visceral leishmaniasis DIAGNÓSTICO LABORATORIAL E SINAIS CLÍNICOS PARA LEISHMANIOSE VISCERAL CANINA EM CÃES EXAMINADOS NO CENTRO DE CONTROLE DE ZOONOSES DE CAMPO GRANDE -MS

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Comparison of serological and parasitological methods for cutaneous leishmaniasis diagnosis in the state of Paraná, Brazil

Cited by 40 publications

References 25 publications

Epidemiological and clinical characteristics of cutaneous leishmaniasis and their relationship with the laboratory data, south of Brazil

Epidemiological and clinical characteristics of cutaneous leishmaniasis and their relationship with the laboratory data, south of Brazil

Serological and Molecular Investigation of Cutaneous Leishmaniasis in Healthy Individuals from an American Cutaneous Leishmaniasis-Endemic Region

Laboratory Diagnosis and Clinical Signs of Canine Visceral Leishmaniasis in Dogs Examined at the Center for Zoonosis Control in Campo Grande – Ms, Brazil

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