Leishmania infantum infection in dogs from maroon communities in the Eastern Amazon

Silva, Andréia Ferreira da; Damasceno, Ágatha Rossanni Alves; Prado, Welton Seabra; Caldeira, Rossela Damasceno; Sampaio-Júnior, Francisco Dantas; Farias, Diana Maria de; Silva, Laís Cristina Oliveira da; Guimarães, Ricardo José de Paula Souza e; Góes-Cavalcante, Gustavo; Scofield, Alessandra

doi:10.1590/0103-8478cr20160025

Cited by 5 publications

(3 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In these areas wandering dogs, as well as owned restricted and semi-restricted ones are usually part of the domestic fauna. Data on canine zoonotic pathogens from these communities are scarce, with a single report recording a prevalence of 8.4% for L. infantum in skin samples from dogs (Silva et al 2017 ). Nevertheless, the presence of wandering dogs, vectors and deficient sanitary conditions suggest that other pathogens of veterinary and public health concern may occur in these areas.…”

Section: Introductionmentioning

confidence: 99%

Vector-borne pathogens of zoonotic concern in dogs from a Quilombola community in northeastern Brazil

et al. 2022

View full text Add to dashboard Cite

Canine vector-borne pathogens (CVBPs) comprise a group of disease agents mainly transmitted by ticks, fleas, mosquitoes and sand flies. In this study, we assessed the presence of CVBPs in an Afro-descendent community (Quilombola) of northeastern, Brazil. Dog blood samples (n = 201) were collected and analyzed by rapid test for the detection of antibodies against Leishmania spp., Anaplasma spp., Ehrlichia spp. and Borrelia burgdorferi sensu lato (s.l.), and antigens of Dirofilaria immitis. In addition, polymerase chain reactions were performed for Anaplasmataceae, Babesia spp., Hepatozoon spp., Rickettsia spp. and B. burgdorferi s.l. Overall, 66.7% of the dogs scored positive to at least one pathogen at serological and/or molecular methods. Antibodies against Ehrlichia spp. were the most frequently detected (57.2%; n = 115/201), followed by Anaplasma spp. (8.5%; n = 17/201), Leishmania spp. (8.5%; n = 17/201) and B. burgdorferi s.l. (0.5%; n = 1/201). For D. immitis, 11 out of 201 (5.5%) animals scored positive. At the molecular analysis, 10.4% (n = 21/201) of the samples scored positive for Babesia spp./Hepatozoon spp., followed by Anaplasmataceae (5.0%; n = 10/201) and Rickettsia spp. (3.0%; n = 6/201). All samples were negative for B. burgdorferi s.l. Our data demonstrated the presence of CVBPs in the studied population, with a high seropositivity for Ehrlichia spp. In addition, considering the detection of zoonotic pathogens in dogs and their relationship with people from Quilombola communities, effective control strategies are advocated for minimizing the risk of infection in this socially vulnerable human population and their pets.

show abstract

Section: Introductionmentioning

confidence: 99%

Vector-borne pathogens of zoonotic concern in dogs from a Quilombola community in northeastern Brazil

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Moreover, RV1/RV2 primer pairs could also efficiently detect the genetic material of donovani complex in Brazilian dog samples and exhibited high sensitivity primarily due to high kDNA minicircle copy number of the target employed (Almeida et al, 2013;Bigeli, Oliveira Júnior, & Teles, 2012;A. F. Silva et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Applications of polymerase chain reaction for the detection of equine Leishmania sp. infection

Escobar

Döwich

Cantele

et al. 2020

SCA

View full text Add to dashboard Cite

Leishmaniasis is a neglected zoonotic disease caused by a variety of pathogenic Leishmania species. In the New World, especially in Brazil, visceral leishmaniasis (VL) is caused by Leishmania infantum. The pathogen can infect several animal species including dogs, foxes, rodents, primates, felines, equines and humans. Dogs act as the primary domestic reservoirs. This study aimed to use polymerase chain reaction (PCR) for detecting Leishmania infection in horses living in a canine visceral leishmaniasis (CVL) endemic region. DNA samples from horse peripheral blood were used to perform PCR. Templates were amplified using primers for the kinetoplast DNA (kDNA) minicircles, which were able to detect different species of Leishmania. In addition, primers for internal transcribed spacer (ITS) of ribosomal DNA were used for detection of Trypanosomatidae sp. Amongst the 75 (39%) positive PCR samples from total 192 samples, 21 samples were positive for kDNA and 63 samples were positive for either ITS, ITS1, or ITS2 gene markers. The kDNA PCR and sequencing allowed the detection of L. infantum in horse blood samples. To our knowledge, this is the first report of equine infection with L. infantum in Southern Brazil. These results proved that L. infantum could also infect horses in addition to humans and dogs, as well as in European countries. This conclusion emphasizes the urgent need to follow up investigation of the infection in these animals.

show abstract

“…Percebe-se que a sensibilidade da técnica de diagnóstico parasitológico depende de algumas variáveis, como o grau do parasitismo, entre outras (LAURENTI, 2009). Inhangapi, Pará, foi possível detectar a infecção por L. infantum em cães (SILVA et al, 2017). Estes dados mostram a importância da caracterização da espécie de Leishmania, visto que, corrobora com o direcionamento das ações de controle da parasitose em área de transmissão.…”

Section: Inquérito Humano Na áRea Urbanaunclassified

Análise Da Leishmaniose Visceral Canina E Humana No Município De Cláudio, Macrorregional De Saúde Oeste De Minas Gerais, Brasil

Lamounier¹,

Lopes²,

Gama-Melo³

et al. 2020

Hygeia

View full text Add to dashboard Cite

A Organização Mundial de Saúde e o Ministério da Saúde do Brasil preconizam a importância de avaliar a ocorrência de doenças negligenciadas, entre elas as Leishmanioses, em regiões consideradas indenes, pois a identificação precoce de áreas de ocorrência dessas enfermidades favorece a implementação de medidas preventivas de forma mais eficaz. Objetivo: Determinar a prevalência de leishmaniose visceral (LV) canina, a frequência de LV humana e identificar espécies de Leishmania circulantes na cidade de Cláudio, região Centro Oeste de Minas Gerais. Método: Foi realizado inquérito sorológico amostral canino na área urbana do município utilizando dois testes imunológicos (imunocromatografia rápida, ensaio imunoenzimático), e diagnóstico sorológico em humanos por três testes (imunocromatografia rápida, ensaio imunoenzimático, imunofluorescência indireta) em residentes de casas com cães parasitados e/ou onde foram capturados Lutzomyia longipalpis, inseto vetor de Leishmania infantum. Resultados: Dos 430 cães examinados, cinco foram sororreagentes nos dois testes imunológicos, com prevalência de 1,2% (IC95%: 0,4%–2,7%). Nas amostras de medula óssea, baço e linfonodos dos cães sororreagentes, foram identificadas formas amastigotas e através do diagnóstico molecular constatou a presença de L. infantum, parasito causador da leishmaniose visceral. Na pesquisa da Leishmaniose visceral humana foram avaliados 74 indivíduos, não sendo identificado nenhum com sorologia positiva. Conclusão: É muito importante a implantação de estratégias de prevenção e controle da leishmaniose visceral canina no município, evitando a propagação da parasitose entre a população canina e consequentemente a transmissão para população humana, pois essas enfermidades estão em franca expansão na região Centro Oeste de Minas Gerais, e em humanos pode levar ao óbito se não diagnosticada e tratada precocemente.

show abstract

Leishmania infantum infection in dogs from maroon communities in the Eastern Amazon

Abstract: This study was designed to detect L. infantum infection in dogs and to evaluate the factors associated with canine visceral leishmaniasis in the maroon communities of Menino Jesus de Petimandeua and

Cited by 5 publications

References 11 publications

Vector-borne pathogens of zoonotic concern in dogs from a Quilombola community in northeastern Brazil

Vector-borne pathogens of zoonotic concern in dogs from a Quilombola community in northeastern Brazil

Applications of polymerase chain reaction for the detection of equine Leishmania sp. infection

Análise Da Leishmaniose Visceral Canina E Humana No Município De Cláudio, Macrorregional De Saúde Oeste De Minas Gerais, Brasil

Contact Info

Product

Resources

About