Leishmania donovani: Intraspecific Polymorphisms of Sudanese Isolates Revealed by PCR-based Analyses and DNA Sequencing

Tai, Nahla O. El; Fari, Mustafa El; Maurício, Isabel; Miles, Michael A.; Oskam, Linda; Safi, Sayda Hassan El; Presber, W; Schönian, Gabriele

doi:10.1006/expr.2001.4592

Cited by 135 publications

(84 citation statements)

References 27 publications

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“…Linking the polymorphism of Leishmania samples with clinical outcomes has not always been evident in the past (19,37,44,47,52). As highlighted by Schriefer et al (48), the wide geographic distribution and multiple sources (e.g., vectors, reservoirs, and human immunodeficiency virus-positive human hosts) of the isolates tested may be among the reasons for this lack of association.…”

Section: Discussionmentioning

confidence: 99%

“…Because of the high level of polymorphism in the composition of the ITS locus, several molecular epidemiological studies have been conducted by RFLP analysis in New World taxa (5,10,21,38), but most studies have been performed on Old World Leishmania species (7,19,34,(44)(45)(46)(47). Analyses of the ribosomal ITS regions of different parasites have demonstrated the utility of this marker in detecting genetic diversity and any association of this diversity with several epidemiological aspects (16,33).…”

Section: Discussionmentioning

confidence: 99%

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Molecular Epidemiology of Leishmania ( Viannia ) guyanensis in French Guiana

et al. 2006

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Little information is available about the genetic variability of Leishmania populations and the possible correlations with ecoepidemiological features of leishmaniases. The present study was carried out in French Guiana, a country where cutaneous leishmaniases (CL) are endemic over the whole territory. The genetic polymorphism of a nuclear sequence encompassing the end of the ribosomal small subunit and the internal transcribed spacer 1 of 265 isolates from patients with CL was examined by restriction fragment length polymorphism analysis. Genotypes based on the fingerprinting phenetic integration were compared to epidemiological, clinical, and geographical data. In agreement with previous reports, five different Leishmania species were identified, but Leishmania (Viannia) guyanensis represented 95.8% of the samples. Two distinct L. (V.) guyanensis populations were found to originate in two ecologically characterized regions. Higher lesional parasite densities and the need for additional treatments were significantly linked to genotype group I. Parasites of genotype group II were more likely to cause chronic and disseminated cutaneous forms in patients. L. (V.) guyanensis was previously said not to be very polymorphic; however, the present analysis resulted in a significant degree of discrimination among L. (V.) guyanensis isolates from diverse ecological areas and with different clinical implications.In the highly biodiversified region of the New World (NW) that encompasses the northern Amazon Basin and the Guiana Shield, at least seven named species belonging to the Leishmania genus coexist with often overlapping hosts and vectors (39). American visceral leishmaniases due to Leishmania (Leishmania) infantum are found there, but cases of cutaneous leishmaniases (CL) are far more numerous. Human cutaneous infections may be unapparent; however, many of the Leishmania parasites are able to produce a spectrum of diseases rather than a single clinical form. Cases reported in that region range from localized and diffuse cutaneous forms to mucocutaneous leishmaniases. They are now being reported in areas where leishmaniases had previously not been endemic (2, 17): increasing risk factors related to natural and especially manmade environmental changes are making leishmaniases a growing public health concern in this particular region. However, little information is available about the genetic variability of parasite populations and the possible correlation with ecoepidemiological features of the diseases. The present study was carried out in French Guiana, a country where CL are endemic over the whole territory (incidence of 0. 2% between 1979 and 2000 [6, 14]). The genetic polymorphism of a nuclear sequence encompassing the end of the ribosomal small subunit (SSU) and the internal transcribed spacer 1 (ITS1) of 265 samples from patients with CL was examined by restriction fragment length polymorphism (RFLP) analysis. Genotypes based on the fingerprinting phenetic integration were then compared to epidemiological, clin...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Molecular Epidemiology of Leishmania ( Viannia ) guyanensis in French Guiana

et al. 2006

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“…Earlier studies to differentiate between VL and PKDL patient isolates in Sudan did not reveal any correlation between sequence polymorphisms and clinical manifestations of human disease (3,7). No such studies have been reported with isolates of L. donovani from Indian populations.…”

Section: Leishmania Donovani In India Causes Visceral Infection (Kalamentioning

confidence: 94%

DNA Polymorphism Assay Distinguishes Isolates ofLeishmania donovaniThat Cause Kala-Azar from Those That Cause Post-Kala-Azar Dermal Leishmaniasis in Humans

et al. 2004

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“…Meanwhile, DNA-based methods have replaced traditional methods to analyze the phylogenetic relationship among species with various targets such as kDNA genes, introns and nuclear genes (Piarroux et al 1995;Noyes et al 1997;El Tai et al 2001;Ibrahim and Barker 2001;Fraga et al 2010), as they trend to be more specific and stable. These studies suggest that the taxonomy of the L. donovani complex needs to be revised (Lukeš et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Phylogenic analysis of Chinese Leishmania isolates based on small subunit ribosomal RNA (SSU rRNA) and 7 spliced leader RNA (7SL RNA)

Wang

DePing

Sun³

et al. 2012

Acta Parasitologica

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The leishmaniases are zoonotic diseases caused by protozoan parasites of the genus Leishmania. Leishmaniases are still endemic in China, especially in the west and northwest froniter regions. To revalue the preliminary phylogenetic results of Chinese Leishmania isolates, we amplified partial fragment of small subunit ribosomal RNA (SSU rRNA) and 7 spliced leader RNA (7SL RNA), then tested the phylogenetic relationships among Chinese Leishmania isolates and their relatives by analyzing SSU rRNA gene sequences and 7SL RNA gene sequences. 19 SSU RNA sequences and 9 7SL RNA sequences were obtained in our study, then analyzed with 42 SSU RNA sequences and 32 7SL RNA sequences retrieved from Genbank, respectively. In the Bayesian analysis of the SSU RNA gene, the isolate

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Leishmania donovani: Intraspecific Polymorphisms of Sudanese Isolates Revealed by PCR-based Analyses and DNA Sequencing

Cited by 135 publications

References 27 publications

Molecular Epidemiology of Leishmania ( Viannia ) guyanensis in French Guiana

Molecular Epidemiology of Leishmania ( Viannia ) guyanensis in French Guiana

DNA Polymorphism Assay Distinguishes Isolates ofLeishmania donovaniThat Cause Kala-Azar from Those That Cause Post-Kala-Azar Dermal Leishmaniasis in Humans

Phylogenic analysis of Chinese Leishmania isolates based on small subunit ribosomal RNA (SSU rRNA) and 7 spliced leader RNA (7SL RNA)

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