Leishmania chagasi in Opossums (Didelphis albiventris) in an Urban Area Endemic for Visceral Leishmaniasis, Campo Grande, Mato Grosso do Sul, Brazil

Humberg, Roberta Martins Passos; Oshiro, Elisa Teruya; Cruz, Maria do Socorro Pires e; Ribolla, Paulo Eduardo Martins; Alonso, Diego Peres; Ferreira, Alda Maria Teixeira; Bonamigo, Raquel de Abreu; Tasso, Norton; Oliveira, Alessandra Gutierrez de

doi:10.4269/ajtmh.2012.11-0534

Cited by 30 publications

(25 citation statements)

References 14 publications

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“…Cats and a different species of opossum ( M. domestica ) did not support sand fly development at all, and dogs were intermediate in their abilities to efficiently support the sand fly life cycle. These observations raise hypotheses about animals that could serve as potential reservoir hosts for Leishmania species (Humberg et al 2012). Recent studies have found evidence of VL in cats (Maia and Campino 2011, Vides JP et al 2011).…”

Section: Discussionmentioning

confidence: 98%

Feeding Preferences ofLutzomyia longipalpis(Diptera: Psychodidae), the Sand Fly Vector, forLeishmania infantum(Kinetoplastida: Trypanosomatidae)

et al. 2014

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Leishmania infantum, the causative agent of visceral leishmaniasis (VL) in Brazil, is spread mostly by the bite of the sand fly Lutzomyia longipalpis (Lutz & Neiva). We trapped sand flies in endemic neighborhoods near Natal, Brazil, where cases of human and dog VL were documented. Amplification of species-specific cytochrome b (Cyt b) genes by polymerase chain reaction revealed that sand flies from rural and periurban areas harbored blood from different sources. The most common source of bloodmeal was human, but blood from dog, chicken, and armadillo was also present. We tested the preference for a source of bloodmeal experimentally by feeding L. longipalpis F1 with blood from different animals. There were significant differences between the proportion of flies engorged and number of eggs laid among flies fed on different sources, varying from 8.4 to 19 (P < 0.0001). Blood from guinea pig or horse was best to support sand fly oviposition, but human blood also supported sand fly oviposition well. No sand flies fed on cats, and sand flies feeding on the opossum Monodelphis domestica Wagner produced no eggs. These data support the hypothesis that L. longipalpis is an eclectic feeder, and humans are an important source of blood for this sand fly species in periurban areas of Brazil.

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Section: Discussionmentioning

confidence: 98%

Feeding Preferences ofLutzomyia longipalpis(Diptera: Psychodidae), the Sand Fly Vector, forLeishmania infantum(Kinetoplastida: Trypanosomatidae)

et al. 2014

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“…In a similar study performed in Minas Gerais, five of 20 Didelphis marsupialis were PCR positive for Leishmania sp., and 24 out of 111 were positive by IFAT (Schallig et al 2007). L. infantum DNA was detected by PCR in 15 out of 54 Didelphis albiventris opossums in the State of Mato Grosso do Sul, Brazil (Humberg et al 2012). Leishmania sp.…”

Section: Discussionmentioning

confidence: 99%

Diagnosis of Leishmania infantum infection by Polymerase Chain Reaction in wild mammals

et al. 2014

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Pesq. Vet. Bras. 34(12):1243-1246, dezembro 2014 1243 RESUMO.-[Diagnóstico de infecção por Leishmania infantum pela reação em cadeia da polimerase em mamíferos silvestres.] A leishmaniose visceral é uma doença infecciosa crônica de mamíferos causada, no Brasil, pelo protozoário Leishmania infantum (sinonímia: Leishmania chagasi) e transmitida pelo flebótomo Lutzomyia longipalpis. Trata-se de uma zoonose endêmica em muitas regiões do Brasil, inclusive em Belo Horizonte, Minas Gerais. Em centros urbanos, leishmaniose visceral acomete principalmente o cão doméstico. Entretanto, L. infantum já foi diagnosticada em outras espécies, incluindo canídeos e primatas de cativeiro em zoológicos. Este estudo buscou avaliar a presença do DNA deste agente em animais de cativeiro e de vida livre da Fundação Zoobotânica de Belo Horizonte através da reação em cadeia da polimerase. Foram analisadas oitenta e uma amostras de sangue oriundas de primatas, carnívoros, ruminantes, edentatos, marsupial e herbívoro de estômago simples. Três primatas Alouatta guariba (bugio marrom) e dois canídeos Speothos venaticus (cachorro--do-mato-vinagre), foram positivos, demonstrando a importância do controle da leishmaniose em áreas endêmicas com a finalidade de conservar a fauna silvestre mantida em cativeiro.

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“…Previous studies showed that the presence of opossum (Didelphis marsupialis) was an important risk factor for the transmission of leishmaniasis, since opossum populations usually have high infection rates in urban endemic area, suggesting that this species may act as a natural link between domestic and sylvatic Leishmania life cycles, especially because opossums and dogs can inhabit the same environment [37,38]. Moreover, opossum infection by L. infantum chagasi has been associated with human VL in some areas [36].…”

Section: Discussionmentioning

confidence: 99%

“…The search for leishmaniasis in opossum is justified by reports of natural infection of this animal in different regions of Brazil [12,35,36]. Previous studies showed that the presence of opossum (Didelphis marsupialis) was an important risk factor for the transmission of leishmaniasis, since opossum populations usually have high infection rates in urban endemic area, suggesting that this species may act as a natural link between domestic and sylvatic Leishmania life cycles, especially because opossums and dogs can inhabit the same environment [37,38].…”

Section: Discussionmentioning

confidence: 99%

Survey of Leishmania Infantum Chagasi in Wild and Domestic Animals in Urban Area and Atlantic Rainforest Fragment in Northeast, Brazil

Costa¹,

Ferreira²,

Fournier³

et al. 2014

J Biodivers Biopros Dev

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Objective: Survey of Leishmania infantum chagasi in domestic and wild mammals in urban area and a Biological Reserve in Natal, Rio Grande do Norte, Brazil.Methods: Domestic and wild mammals were captured in Brazilian northeast Atlantic Rainforest, Rio Grande do Norte state. Serological and parasitological studies were conducted; Leishmania isolates were positioned in phylogeny based on small subunit rDNA (SSU rDNA) and glycosomal-3-phosphate dehydrogenase (gGAPDH) gene sequences.Results: Blood samples were collected from 138 wild and domestic mammals, comprising 66 dogs, 52 cats and 20 marsupials. Antibodies were found in 27 dogs (40.91%), two cats (3.85%) and one Didelphis albiventris (5%). The cultures of popliteal lymph node aspirates were positive in nine dogs (13.64%) and from seven (10.61%) the parasite was isolated and cryopreserved. All isolates were positioned in phylogeny based on SSU rDNA and gGAPDH in the same branch with L. infantum chagasi. Conclusions:The proximity of the forest fragment with humans and their domestic animals provide interference in the health of wild animals. Measures to control the population of feral cats and environmental conservation should be implemented due the importance of visceral leishmaniasis.

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Leishmania chagasi in Opossums (Didelphis albiventris) in an Urban Area Endemic for Visceral Leishmaniasis, Campo Grande, Mato Grosso do Sul, Brazil

Cited by 30 publications

References 14 publications

Feeding Preferences ofLutzomyia longipalpis(Diptera: Psychodidae), the Sand Fly Vector, forLeishmania infantum(Kinetoplastida: Trypanosomatidae)

Feeding Preferences ofLutzomyia longipalpis(Diptera: Psychodidae), the Sand Fly Vector, forLeishmania infantum(Kinetoplastida: Trypanosomatidae)

Diagnosis of Leishmania infantum infection by Polymerase Chain Reaction in wild mammals

Survey of Leishmania Infantum Chagasi in Wild and Domestic Animals in Urban Area and Atlantic Rainforest Fragment in Northeast, Brazil

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