Leiomyosarcomas and Benign Smooth Muscle Tumors of the Stomach: Nuclear DNA Patterns Studied by Flow Cytometry

Tsushima, K; Rainwater, Leslie M.; Goellner, John R.; Heerden, Jon A. van; Lieber, Michael M.

doi:10.1016/s0025-6196(12)61904-1

Cited by 46 publications

(16 citation statements)

References 13 publications

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“…In STGIT, tumour ploidy has been analysed by flow cytometry (Cooper et al 1992;Cunningham et al 1993;Kiyabu et al 1988;Tsushima et al 1987) and cytophotometry (Cunningham et al 1993;Federspiel et al 1987;Flint et al 1989). The frequency of aneuploidy varies from 25% (Cooper et al 1992) to 41% (Kiyabu et al 1988) rising to 54% in sarcomas (47% in low-grade and 71% in high-grade sarcomas; Kiyabu et al 1988).…”

Section: Discussionmentioning

confidence: 99%

“…Tumour size appears to have some prognostic value, whereas cellularity and nuclear pleomorphism are considered less important (Hendrickson and Kempson 1989). Although there is some disagreement (Flint et al 1989), recent studies have shown that ploidy of STGIT is related to microscopic features (Cooper et al 1992;Federspiel et al 1987;Kiyabu et al 1988;Tsushima et al 1987) and survival (Cooper et al 1992;Kiyabu et al 1988;Tsushima et al 1987).…”

Section: Introductionmentioning

confidence: 99%

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Stromal tumours of the gastrointestinal tract: a clinicopathological and ploidy analysis of 33 cases

Lerma

Oliva

Tugues

et al. 1994

Vichows Archiv A Pathol Anat

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The clinicopathological and DNA flow cytometric data of 33 patients with stromal tumours of the gastrointestinal tract (STGIT) were analysed to select pathological features of prognostic value. Tumours had been previously classified as benign (21 cases) or malignant (12 cases). Data relating to poor prognosis statistically were local invasion, pathological grade, size greater than 10 cm, mitotic index (MI) and necrosis. Pathological grade was related to local invasion. Aneuploidy did not correlate with poor survival although a common trend was detected between both. DNA content may help to predict prognosis of STGIT, but its real value has not yet been clearly established. Currently, stage (invasion), size, MI and pathological grade remain the most useful prognostic factors.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Stromal tumours of the gastrointestinal tract: a clinicopathological and ploidy analysis of 33 cases

Lerma

Oliva

Tugues

et al. 1994

Vichows Archiv A Pathol Anat

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“…Thus, DNA aneuploidy has been claimed to allow a sensitive and specific discrimination between benign and malignant tumors of the gastrointestinal tract and to correlate with their prognosis [22][23][24][25][26][27][28] . However, in other reports, aneuploidy could not be used as a diagnostic criterion of malignancy, but was associated with a mere tendency to an adverse outcome [29,30] .…”

Section: Discussionmentioning

confidence: 99%

DNA ploidy andc-Kitmutation in gastrointestinal stromal tumors

Lee¹

2004

WJG

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AIM:To investigate the prognostic significance of c-Kit gene mutation and DNA ploidy in gastointestinal stromal tumors (GISTs). METHODS:A total of 55 cases of GISTs were studied for the expression of c-Kit by immunohistochemistry, and the c-Kit gene mutations in exons 9, 11, 13, and 17 were detected by polymerase chain reaction-single strand confirmation polymarphism (PCR-SSCP) and denaturing high performance liquid chromatography (D-HPLC) techniques. DNA ploidy was determined by flow cytometry. RESULTS:Of the 55 cases of GISTs, 53 cases (96.4%) expressed c-Kit protein. The c-Kit gene mutations of exons 11 and 9 were found in 30 (54.5%) and 7 cases (12.7%), respectively. No mutations were found in exons 13 and 17. DNA aneuploidy was seen in 10 cases (18.2%). The c-Kit mutation positive GISTs were larger in size than the negative GISTs. The aneuploidy tumors were statistically associated with large size, high mitotic counts, high risk groups, high cellularity and severe nuclear atypia, and epithelioid type. There was a tendency that c-Kit mutations were more frequently found in aneuploidy GISTs. CONCLUSION:DNA aneuploidy and c-Kit mutations can be considered as prognostic factors in GISTs.Lee JH, Zhang X, Jung WY, Chae YS, Park JJ, Kim I. DNA ploidy and c-Kit mutation in gastrointestinal stromal tumors.

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“…Nevertheless, unequivocal aneuploidy is signifi cantly associated with tumor malignancy. Preliminary data indicate that determination of the DNA ploidy by flow cytometry may be significant for the prognosis of leio myosarcomas [22,23], In this study, flow cytometry was conducted in 46 cases of gastric myogenic tumors. Twelve of 24 leiomyosarcomas were aneuploid and all 22 leiomyo mas were diploid.…”

Section: Discussionmentioning

confidence: 99%

Usefulness of Nucleolar Organizer Region Staining in Gastric Myogenic Tumors: Correlation with Ploidy by DNA Flow Cytometry

Shimamoto

Haruma²,

Tanaka³

et al. 1993

Oncology

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A silver-staining technique to identify nucleolar organizer region-associated proteins (Ag-NOR) was applied to 55 gastric myogenic tumors. The mean numbers of Ag-NOR in the nucleus were: leiomyoma (30 cases), 2.0 + 0.6 (mean + SD); low-grade leiomyosarcoma (11 cases), 3.0 + 0.7; and high-grade leiomyosarcoma (14 cases), 3.9 + 0.7. The Ag-NOR counts were compared with DNA ploidy as determined by flow cytometry in 46 tumors. The Ag-NOR counts were significantly different in the aneuploid leiomyosarcomas (4.1 + 0.6: mean + SD) and diploid leiomyosarcomas (2.9 + 0.6: mean + SD). Thus, this parameter may serve as an objective histological discriminant for malignancy.

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Leiomyosarcomas and Benign Smooth Muscle Tumors of the Stomach: Nuclear DNA Patterns Studied by Flow Cytometry

Cited by 46 publications

References 13 publications

Stromal tumours of the gastrointestinal tract: a clinicopathological and ploidy analysis of 33 cases

Stromal tumours of the gastrointestinal tract: a clinicopathological and ploidy analysis of 33 cases

DNA ploidy andc-Kitmutation in gastrointestinal stromal tumors

Usefulness of Nucleolar Organizer Region Staining in Gastric Myogenic Tumors: Correlation with Ploidy by DNA Flow Cytometry

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