Lectins as potential tools for cancer biomarker discovery from extracellular vesicles

Islam, Md. Khirul; Khan, Misba; Gidwani, Kamlesh; Witwer, Kenneth W.; Lamminmäki, Urpo; Leivo, Janne

doi:10.1186/s40364-023-00520-6

Cited by 6 publications

(4 citation statements)

References 195 publications

(215 reference statements)

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“…Lectins, which are carbohydrate-binding proteins that recognize specific glycans, can efficiently label EVs through their major carbohydrate-recognition domains. WGA is an example of such lectins ( 25 ). We previous introduced an innovative, user-friendly and highly efficient technique for the capture and quantitative analysis of EVs by leveraging WGA-coupled magnetic beads in conjunction with flow cytometry ( 19 ).…”

Section: Resultsmentioning

confidence: 99%

ECM1 and ANXA1 in urinary extracellular vesicles serve as biomarkers for breast cancer

Huang,

Wan,

et al. 2024

Front. Oncol.

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ObjectiveAlthough urinary extracellular vesicles (uEVs) have been extensively studied in various cancers, their involvement in breast cancer (BC) remains largely unexplored. The non-invasive nature of urine as a biofluid and its abundant protein content offer considerable potential for the early detection of breast cancer.MethodsThis study analyzed the proteomic profiles of uEVs from BC patients and healthy controls (HC). The dysregulation of ECM1 and ANXA1 in the uEVs was validated in a larger cohort of 128 BC patients, 25 HC and 25 benign breast nodules (BBN) by chemiluminescence assay (CLIA). The expression levels of ECM1 and ANXA1 were also confirmed in the uEVs of MMTV-PyMT transgenic breast cancer mouse models.ResultsLC-MS/MS analysis identified 571 dysregulated proteins in the uEVs of BC patients. ECM1 and ANXA1 were selected for validation in 128 BC patients, 25 HC and 25 BBN using CLIA, as their fold change showed a significant difference of more than 10 with p-value<0.05. Protein levels of ECM1 and ANXA1 in uEVs were significantly increased in BC patients. In addition, the protein levels of ECM1 and ANXA1 in the uEVs of MMTV-PyMT transgenic mice were observed to increase progressively with the progression of breast cancer.ConclusionWe developed a simple and purification-free assay platform to isolate uEVs and quantitatively detect ECM1 and ANXA1 in uEVs by WGA-coupled magnetic beads and CLIA. Our results suggest that ECM1 and ANXA1 in uEVs could potentially serve as diagnostic biomarkers for breast cancer.

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Section: Resultsmentioning

confidence: 99%

ECM1 and ANXA1 in urinary extracellular vesicles serve as biomarkers for breast cancer

Huang,

Wan,

et al. 2024

Front. Oncol.

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“…Viable biomolecules in cancer therapy have become a promising target in the eld of biotechnology. Carbohydrate-speci c binding proteins play an important therapeutic and biotechnological role (Islam et al, 2023). Lectins can recognize carbohydrate or glycoconjugate fractions and bind reversibly to cancer cells with high speci city and without changes in the covalent structure (Figueiroa et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…In another study, Freitas et al (2019) described that the lectins E-PHA and L-PHA bind to cancer-related N-glycans, and the AAL lectin binds to fucose glycans. It is important to highlight that glycoconjugates present in extracellular vesicles play versatile roles in cancer (Islam et al, 2023). A study by Surman et al (2018) showed that a group of lectins can bind speci c glycan epitopes in extracellular vesicles compared to parental cell membranes.…”

Section: Discussionmentioning

confidence: 99%

Genipa americana lectin (GaBL) induces inhibition of growth, migration, invasion, and regulation of the expression of caspase-mediated apoptosis and proteins related to the development of cancer in human head and neck cells

Costa,

Silva,

Gomes

et al. 2024

Preprint

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The antitumor activity of Genipa americana bark lectin (GaBL) was evaluated for the first time against cell lines of human skin cancer (A431), melanoma (B16), and squamous cell carcinoma of the tongue (SCC9). Cancer cell lines were treated with 10 µg/ml of GaBL to assess cell viability, cell migration and invasion, as well as the identification of cell membrane alterations associated with apoptosis. Real-time polymerase chain reaction for caspase-3 was performed to verify if apoptosis is activated by lectin treatment. The mRNA expression of proteins (E-cadherin, type I collagen) related to the epithelial-mesenchymal transition was also analyzed. GaBL decreased (27.5–50%) cell proliferation and reduced cell migration in all strains evaluated. Additionally, the lectin decreased the invasion of SCC9 cells. Apoptosis was higher against B16 and SCC9 cells treated with the lectin. GaBL induced the upregulation of caspase-3, E-cadherin and suppression of type I collagen in all strains tested, indicating lower cancer development. GaBL induces inhibition of growth, migration, invasion, and regulation of the expression of caspase-mediated apoptosis and proteins related to the development of cancer in human head and neck cells.

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“…This leverages its preferential binding to malignant cells to guide nanostructured systems containing conventional drugs to the tumor, thereby reducing undesired side-effects [67,68]. Additionally, Cvill can serve as a tool for glycomics and glycoproteomics studies, facilitating the investigation and detection of glycosylation profiles on different cancer cell lines by exploiting the various glycans present on the cell surface [69][70][71]. This enables the use of the lectin as a diagnostic tool and in cancer biomarker research.…”

Section: Discussionmentioning

confidence: 99%

Structural Analysis and Characterization of an Antiproliferative Lectin from Canavalia villosa Seeds

Lossio,

Osterne,

Pinto-Junior

et al. 2023

IJMS

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Cells use glycans to encode information that modulates processes ranging from cell–cell recognition to programmed cell death. This information is encoded within a glycocode, and its decoding is performed by carbohydrate-binding proteins. Among these, lectins stand out due to their specific and reversible interaction with carbohydrates. Changes in glycosylation patterns are observed in several pathologies, including cancer, where abnormal glycans are found on the surfaces of affected tissues. Given the importance of the bioprospection of promising biomolecules, the current work aimed to determine the structural properties and anticancer potential of the mannose-specific lectin from seeds of Canavalia villosa (Cvill). Experimental elucidation of the primary and 3D structures of the lectin, along with glycan array and molecular docking, facilitated the determination of its fine carbohydrate-binding specificity. These structural insights, coupled with the lectin’s specificity, have been combined to explain the antiproliferative effect of Cvill against cancer cell lines. This effect is dependent on the carbohydrate-binding activity of Cvill and its uptake in the cells, with concomitant activation of autophagic and apoptotic pathways.

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Lectins as potential tools for cancer biomarker discovery from extracellular vesicles

Cited by 6 publications

References 195 publications

ECM1 and ANXA1 in urinary extracellular vesicles serve as biomarkers for breast cancer

ECM1 and ANXA1 in urinary extracellular vesicles serve as biomarkers for breast cancer

Genipa americana lectin (GaBL) induces inhibition of growth, migration, invasion, and regulation of the expression of caspase-mediated apoptosis and proteins related to the development of cancer in human head and neck cells

Structural Analysis and Characterization of an Antiproliferative Lectin from Canavalia villosa Seeds

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