Lectin-magnetic separation can enhance methods for the detection of Staphylococcus aureus, Salmonella enteritidis and Listeria monocytogenes

Payne, Mary; Campbell, Shona; Kroll, R.G.

doi:10.1006/fmic.1993.1008

Cited by 18 publications

(5 citation statements)

References 15 publications

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“…It was used in the development of an impedimetric biosensor based on self-assembled hybrid cysteine-gold nanoparticles for the analysis of S. enterica [48]. Also, a Triticum vulgaris lectin (36 kDa), with an affinity for glycoconjugates with N-acetylglucosamine or N-acetylneuraminic acid residues, was used for magnetic separation of S. enterica [49].…”

Section: Lectin Evaluationmentioning

confidence: 99%

Impedimetric Biosensor Based on a Hechtia argentea Lectin for the Detection of Salmonella spp.

et al. 2020

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A sensitive electrochemical detection method for Salmonella spp. was described, based on the use of Hechtia argentea lectin immobilised on a screen-printed gold electrode. The lectin was extracted from Hechtia argentea, a plant belonging to the Bromeliaceae family. The lectin with molecular weight near 27.4 kDa showed selectivity towards D-mannose, contained on the lipopolysaccharide cell wall of Salmonella spp. Carbohydrate selectivity of the lectin was measured as a change in impedance with respect to concentration. The binding of the bacteria to the biosensor surface increased impedance with increasing concentrations of Salmonella spp., achieving a linear range of detection of 15–2.57 × 107 CFU mL−1, with a limit of detection of 5 CFU mL−1. Increases in impedance were measured using electrochemical impedance spectroscopy and analysed using Nyquist plots. The biosensor was applied in analysis of hen egg samples, and the results were consistent with those obtained using the official analysis methodology.

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Section: Lectin Evaluationmentioning

confidence: 99%

Impedimetric Biosensor Based on a Hechtia argentea Lectin for the Detection of Salmonella spp.

et al. 2020

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“…Specific cell recovery was found to be variable, but the use of lectins may offer some promise as an alternative cell discriminator (Payne et al, 1993;. Recovered cell populations were free from environmental impurities and a high percentage of the culturable cells was extracted.…”

Section: Interaction With Magnetic Particles Bearing Immobilized Biolmentioning

confidence: 99%

Magnetic modification of cells

Šafařı́k

Pospíšková

Baldíková³

et al. 2016

Engineering of Nanobiomaterials

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“…Common foodborne pathogens that cause illnesses such as Salmonella spp., S. aureus , and Listeria spp. often coexist with foods, such as in milk and egg . Besides, owing to the fast and continuous growth, for instance, 10 4 –10 5 CFU/h in eggs, multiple pathogens require simultaneous detection as early as possible to avoid potential cross-contamination, even after the food product entered the distribution, where sophisticated approaches are unavailable.…”

Section: Introductionmentioning

confidence: 99%

“…often coexist with foods, 7 such as in milk and egg. 8 Besides, owing to the fast and continuous growth, for instance, 10 4 −10 5 CFU/h in eggs, multiple pathogens require simultaneous detection as early as possible to avoid potential cross-contamination, even after the food product entered the distribution, where sophisticated approaches are unavailable. The sensitive and multiplex detection of foodborne in a simple, fast approach is still challenging and thus remains a high-priority work in estimating the burden of foodborne illness.…”

Section: Introductionmentioning

confidence: 99%

Artificial Intelligence-Based Imaging Transcoding System for Multiplex Screening of Viable Foodborne Pathogens

et al. 2023

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Multiplex detection of viable foodborne pathogens is critical for food safety and public health, yet current assays suffer trade-offs between cost, assay complexity, sensitivities, and the specificity between live and dead bacteria. We herein developed a sensing method using artificial intelligence transcoding (SMART) for rapid, sensitive, and multiplex profiling of foodborne pathogens. The assay utilizes the programmable polystyrene (PS) microspheres to encode different pathogens, inducing subsequent visible signals under conventional microscopy that can be analyzed using a customized, artificial intelligence-computer vision, which was trained to decode the intrinsic properties of PS microspheres to reveal the numbers and types of pathogens. Our approach enabled the rapid and simultaneous detection of multiple bacteria from egg samples of <102 CFU/mL without DNA amplification and showed strong consistency with the standard microbiologic and genotypic methods. We adopted our assay through phage-guided targeting to enable the discrimination between live and dead bacteria.

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Lectin-magnetic separation can enhance methods for the detection of Staphylococcus aureus, Salmonella enteritidis and Listeria monocytogenes

Cited by 18 publications

References 15 publications

Impedimetric Biosensor Based on a Hechtia argentea Lectin for the Detection of Salmonella spp.

Impedimetric Biosensor Based on a Hechtia argentea Lectin for the Detection of Salmonella spp.

Magnetic modification of cells

Artificial Intelligence-Based Imaging Transcoding System for Multiplex Screening of Viable Foodborne Pathogens

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