Impedimetric Biosensor Based on a Hechtia argentea Lectin for the Detection of Salmonella spp.

Lopez-Tellez, Jorge; Sánchez-Ortega, Irais; Hornung‐Leoni, Claudia T.; Santos, Eva M.; Miranda, José M.; Rodríguez, José A.

doi:10.3390/chemosensors8040115

Cited by 7 publications

(3 citation statements)

References 61 publications

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“…From the experimental results, the measured impedance obtained increased with the concentration of Salmonella bound to the biosensor surface and it was linear from 15 to 2.57×107 CFU/mL, with a low limit of 5 CFU/mL. The biosensor was used for the detection of Salmonella in egg samples and the results obtained were consistent with the official test results [60]. Besides, the biosensor had the advantages of short detection time and simple operation and had a broad application prospect.…”

Section: Electrochemical Biosesnsors For Salmonella Detection In Food...supporting

confidence: 74%

“…In the study, this extracted lectin was fixed on a screen-printed gold electrode to serve as identification. When the recognition occurred on the electrode, the impedance of electron transferring happened on the surface of the electrode changed because the presence of self-assembled monolayers altered the rate of electron transfer and the interface capacitance, ultimately leading to an increase in impedance [60]. The overall detection scheme is shown in Figure 7a.…”

Section: Electrochemical Biosesnsors For Salmonella Detection In Food...mentioning

confidence: 99%

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Biosensor Detection Methods for Salmonella: a Review

Du¹

2023

AETR

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Salmonella is a pathogenic bacterium capable of causing serious food-borne illness, which has caused worldwide concern. It can enter the food supply chain at various stages of production, distribution, and marketing. Due to its high prevalence, it is necessary to develop effective and efficient methods for the identification, detection, and monitoring of Salmonella at an early stage. While traditional detection technologies often have long detection times, complex operation and low sensitivity, emerging biosensor technologies combine novel nanomaterials with signal amplification methods to achieve improved performance. Biosensors show several advantages over traditional assays in terms of specificity, accuracy, and sensitivity and show superiority in rapid response. They are now seen as promising alternative tools since they are accessible and applicable approaches for rapid detection. In recent years, there has been a boom in the development of biosensors for the detection of foodborne Salmonella. This paper focuses on the comparison of new methods for the detection of optical and electrochemical biosensors in recent years, analyzes the different separation strategies and determination methods used, and provides an outlook on the future development of biosensors for the detection of Salmonella.

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Section: Electrochemical Biosesnsors For Salmonella Detection In Food...supporting

confidence: 74%

Section: Electrochemical Biosesnsors For Salmonella Detection In Food...mentioning

confidence: 99%

Biosensor Detection Methods for Salmonella: a Review

Du¹

2023

AETR

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“…Impedance, expressed in terms of current-voltage ratio, reflects the resistance of the circuit under the change of electric field, and is generally measured by electrochemical impedance spectroscopy (EIS) [124]. The ultra-low LOD provided by impedance biosensors is outstanding, and one of the main reasons for its rapid development.…”

Section: Impedimetrymentioning

confidence: 99%

Overview of Rapid Detection Methods for Salmonella in Foods: Progress and Challenges

Wang

Zhang

Tian

et al. 2021

Foods

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Salmonella contamination in food production and processing is a serious threat to consumer health. More and more rapid detection methods have been proposed to compensate for the inefficiency of traditional bacterial cultures to suppress the high prevalence of Salmonella more efficiently. The contamination of Salmonella in foods can be identified by recognition elements and screened using rapid detection methods with different measurable signals (optical, electrical, etc.). Therefore, the different signal transduction mechanisms and Salmonella recognition elements are the key of the sensitivity, accuracy and specificity for the rapid detection methods. In this review, the bioreceptors for Salmonella were firstly summarized and described, then the current promising Salmonella rapid detection methods in foodstuffs with different signal transduction were objectively summarized and evaluated. Moreover, the challenges faced by these methods in practical monitoring and the development prospect were also emphasized to shed light on a new perspective for the Salmonella rapid detection methods applications.

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Aptamer based rGO-AuNPs Electrochemical Sensors and its Application for Detection of Salmonella Anatum in Food

Chai¹

2022

International Journal of Electrochemical Science

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Foodborne pathogenic bacteria can cause food poisoning or infection. Salmonella is an aerobic or partly anaerobic gram-negative bacterium that enters the body with food, invades the intestine and releases large amounts of endotoxin. The endotoxin acts as a pyrogen that leads to an increase in body temperature and acts on the intestinal mucosa to trigger systemic inflammation and toxic symptoms in the host. The development of novel methods and systems for the monitoring and detection of foodborne pathogenic bacteria is of great significance for food safety, especially the development of simple, highthroughput and highly sensitive rapid detection techniques. This work proposes a novel aptamer sensor based on reduced graphene oxide (rGO)-AuNPs for the detection of Salmonella in food. Firstly the rGO and AuNPs were modified on the surface of glassy carbon electrode in turn, and then a highly sensitive sensor was made by designing a segment of thiolated aptamer probe that could specifically capture Salmonella. The high electron transport ability of rGO and the advantage of large specific surface area of AuNPs were adopted to achieve signal amplification and improve the detection capability. In the presence of Salmonella, the aptamer traps and immobilizes Salmonella on the surface of the electrode, resulting in a change in the immediate current in the sensor and realizing quantitative detection of Salmonella based on the change in electrical signal. Under the optimized conditions, the response current of the constructed aptamer sensor is linearly related to the logarithmic value of Salmonella concentration, with a linear range of 6×10 2 cfu/mL~6×10 7 cfu/mL and a detection limit of 200 cfu/mL (S/N=3). Meanwhile, this sensor has been successfully applied in the detection of Salmonella in pork and beef.

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Impedimetric Biosensor Based on a Hechtia argentea Lectin for the Detection of Salmonella spp.

Cited by 7 publications

References 61 publications

Biosensor Detection Methods for Salmonella: a Review

Biosensor Detection Methods for Salmonella: a Review

Overview of Rapid Detection Methods for Salmonella in Foods: Progress and Challenges

Aptamer based rGO-AuNPs Electrochemical Sensors and its Application for Detection of Salmonella Anatum in Food

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