Lectin histochemistry of<i>Kudoa septempunctata</i>genotype ST3-infected muscle of olive flounder (<i>Paralichthys olivaceus</i>)

Kang, Jaeyoun; Park, Changnam; Jang, Yeounghwan; Ahn, Meejung; Shin, Taekyun

doi:10.1051/parasite/2016021

Cited by 8 publications

(8 citation statements)

References 16 publications

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“…With development of sequencing technique, numerous genomic researches have been reported to understand infection results by virus 28,31,40 , bactria 26,41,42 , and parasite 43 in the olive flounder genome. A fundamental way to overcome disease outbreak from external pathogens is to approach from their genome level.…”

Section: Discussionmentioning

confidence: 99%

Gene expression profiles alteration after infection of virus, bacteria, and parasite in the Olive flounder (Paralichthys olivaceus)

Nam

Mishra

Gim³

et al. 2018

Sci Rep

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Olive flounder (Paralichthys olivaceus) is one of economically valuable fish species in the East Asia. In comparison with its economic importance, available genomic information of the olive flounder is very limited. The mass mortality caused by variety of pathogens (virus, bacteria and parasites) is main problem in aquaculture industry, including in olive flounder culture. In this study, we carried out transcriptome analysis using the olive flounder gill tissues after infection of three types of pathogens (Virus; Viral hemorrhagic septicemia virus, Bacteria; Streptococcus parauberis, and Parasite; Miamiensis avidus), respectively. As a result, we identified total 12,415 differentially expressed genes (DEG) from viral infection, 1,754 from bacterial infection, and 795 from parasite infection, respectively. To investigate the effects of pathogenic infection on immune response, we analyzed Gene ontology (GO) enrichment analysis with DEGs and sorted immune-related GO terms per three pathogen groups. Especially, we verified various GO terms, and genes in these terms showed down-regulated expression pattern. In addition, we identified 67 common genes (10 up-regulated and 57 down-regulated) present in three pathogen infection groups. Our goals are to provide plenty of genomic knowledge about olive flounder transcripts for further research and report genes, which were changed in their expression after specific pathogen infection.

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Section: Discussionmentioning

confidence: 99%

Gene expression profiles alteration after infection of virus, bacteria, and parasite in the Olive flounder (Paralichthys olivaceus)

Nam

Mishra

Gim³

et al. 2018

Sci Rep

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“…While immunohistochemistry and in situ hybridization methods offer greater specificity and are becoming increasingly cost‐effective, histochemical stains targeting specific cell and tissue components are used most frequently to detect alterations and identify infectious agents in tissues (Bancroft & Stonard, 2013; Smith, Newman, Coleman, & Alex, 2018). Histochemical, lectin histochemical and ultrastructural techniques have been historically used to study myxozoan composition, development and metabolism, particularly the composition of iodinophilous vacuoles and nuclear DNA content (Bajpai & Haldar, 1982; Desser & Paterson, 1978; Kang, Park, Jang, Ahn, & Shin, 2016; Morris & Adams, 2004; Muñoz, Palenzuela, Álvarez‐Pellitero, & Sitjà‐Bobadilla, 1999; Podlipaev & Schulman, 1978; Redondo, Cortadellas, Palenzuela, & Álvarez‐Pellitero, 2008; Uspenskaya, 1975, 1979a, 1982). Myxozoans demonstrate a variety of conserved and differing carbohydrate structures and lectin‐binding patterns, which may be useful in assessing myxozoan function or for diagnostic purposes (Kang et al, 2016; Morris & Adams, 2004; Muñoz et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

“…Histochemical, lectin histochemical and ultrastructural techniques have been historically used to study myxozoan composition, development and metabolism, particularly the composition of iodinophilous vacuoles and nuclear DNA content (Bajpai & Haldar, 1982; Desser & Paterson, 1978; Kang, Park, Jang, Ahn, & Shin, 2016; Morris & Adams, 2004; Muñoz, Palenzuela, Álvarez‐Pellitero, & Sitjà‐Bobadilla, 1999; Podlipaev & Schulman, 1978; Redondo, Cortadellas, Palenzuela, & Álvarez‐Pellitero, 2008; Uspenskaya, 1975, 1979a, 1982). Myxozoans demonstrate a variety of conserved and differing carbohydrate structures and lectin‐binding patterns, which may be useful in assessing myxozoan function or for diagnostic purposes (Kang et al, 2016; Morris & Adams, 2004; Muñoz et al, 1999). The presence of particular carbohydrate moieties can depend on the stage of myxospore development as well as the myxozoan species (Redondo et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Insights into myxozoan composition and physiology revealed by histochemical properties of myxospores

Stilwell

Griffin

Rosser

et al. 2020

Journal of Fish Diseases

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Myxozoa (phylum Cnidaria) are a diverse group of metazoan parasites that predominately infect fish. Little is known regarding the composition and physiology of their myxospore life stage. The objective of this work was to investigate the composition of myxospores and extrasporogonic stages of nine myxozoan species infecting various teleost fish using histochemical staining techniques. Thirty histochemical stains were applied to formalin‐fixed, paraffin‐embedded tissues processed routinely for light microscopic evaluation. The polar capsules were the most consistent stain target across the taxa examined. Polar capsule staining with Alizarin red, von Kossa and methyl green‐pyronin suggests the presence of intracapsular calcium and phosphate, which may contribute to polar filament discharge or pathogenesis of host invasion. The shell valves and suture lines of most myxozoans were stained with Luna and phosphotungstic acid haematoxylin stains, consistent with the presence of chitin and microfibrils, respectively. Vacuoles were consistently highlighted by diastase‐susceptible periodic acid‐Schiff and Grocott's methenamine silver staining, indicating glycogen. Other histochemical stains exhibited inconsistent staining across the taxa, suggesting differences in myxospore composition potentially reflective of physiologic variations and tissue tropisms. This work provides some information on conserved features and taxa‐associated composition of myxospores and lends insight into myxozoan physiology and host–parasite interactions.

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“…However, the immunetriggering mechanism of K. septempunctata infection has not yet been established. http://bmbreports.org BMB Reports In a recent study, lectin histochemistry for K. septempunctata-infected olive flounder revealed the presence of various sugar residues (N-acetylglucosamine [GlcNAc], mannose, galactose and fucose) in Kudoa spores (10). Lectins are glycoproteins that specifically bind carbohydrate structures and potentially participate in biodefense mechanisms on the fish side (11).…”

Section: Introductionmentioning

confidence: 99%

Immune-triggering effect of the foodborne parasite Kudoa septempunctata through the C-type lectin Mincle in HT29 cells

et al. 2020

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Kudoa septempunctata is a myxozoan parasite that causes food poisoning in individuals consuming olive flounder. The present study aimed to investigate the currently insufficiently elucidated early molecular mechanisms of inflammatory responses in the intestine owing to parasite ingestion. After Kudoa spores were isolated from olive flounder, HT29 cells were exposed to spores identified to be alive using SYTO-9 and propidium iodide staining or to antigens of Kudoa spores (KsAg). IL-1, IL-8, TNF- and NFKB1 expression and NF-B activation were assessed using real-time PCR, cytokine array and western blotting. The immunofluorescence of FITC-conjugated lectins, results of ligand binding assays using Mincle-Fc and IgG-Fc, CLEC4E expressions in response to KsAg stimulation, and Mincle-dependent NF-B activation were assessed to clarify the early immunetriggering mechanism. Inflammatory cytokines (IL-1, GM-CSF and TNF-), chemokines (IL-8, CCL2, CCL5 and CXCL1) and NF-B activation (pNF-B/NF-B) in HT29 cells increased following stimulation by KsAg. The immunofluorescence results of spores and lectins (concanavalin A and wheat germ agglutinin) suggested the importance of Mincle in molecular recognition between Kudoa spores and intestinal cells. Practically, data for Mincle-Fc and KsAg binding affinity, CLEC4E mRNA expression, Mincle immunofluorescence staining and hMincledependent NF-B activation demonstrated the involvement of Mincle in the early immune-triggering mechanism. The present study newly elucidated that the molecular recognition and immune-triggering mechanism of K. septempunctata are associated with Mincle on human intestinal epithelial cells. [BMB Reports 2020; 53(9): 478-483]

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Lectin histochemistry ofKudoa septempunctatagenotype ST3-infected muscle of olive flounder (Paralichthys olivaceus)

Cited by 8 publications

References 16 publications

Gene expression profiles alteration after infection of virus, bacteria, and parasite in the Olive flounder (Paralichthys olivaceus)

Gene expression profiles alteration after infection of virus, bacteria, and parasite in the Olive flounder (Paralichthys olivaceus)

Insights into myxozoan composition and physiology revealed by histochemical properties of myxospores

Immune-triggering effect of the foodborne parasite Kudoa septempunctata through the C-type lectin Mincle in HT29 cells

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