Gene expression profiles alteration after infection of virus, bacteria, and parasite in the Olive flounder (Paralichthys olivaceus)

Nam, Gyu-Hwi; Mishra, Anshuman; Gim, Jeong‐An; Lee, Hee-Eun; Jo, Ara; Yoon, Dahye; Kim, Ahran; Kim, Woo Jin; Ahn, Kung; Kim, Do-Hyung; Kim, Suhkmann; Cha, Hee‐Jae; Choi, Yung Hyun; Park, Chan-Il; Kim, Heui‐Soo

doi:10.1038/s41598-018-36342-y

Cited by 12 publications

(4 citation statements)

References 77 publications

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“…Viruses have also been shown to alter the levels of various ECM proteins in multiple cell types [54–56]. Our data revealed a significant reduction in proteins involved in cell adhesion, focal adhesion, cell migration and ECM organization in infected cells.…”

Section: Discussionmentioning

confidence: 55%

Proteomic landscape of Japanese encephalitis virus-infected fibroblasts

Sharma

Chhabra

Aggarwal

et al. 2021

Journal of General Virology

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Advances in proteomics have enabled a comprehensive understanding of host–pathogen interactions. Here we have characterized Japanese encephalitis virus (JEV) infection-driven changes in the mouse embryonic fibroblast (MEF) proteome. Through tandem mass tagging (TMT)-based mass spectrometry, we describe changes in 7.85 % of the identified proteome due to JEV infection. Pathway enrichment analysis showed that proteins involved in innate immune sensing, interferon responses and inflammation were the major upregulated group, along with the immunoproteasome and poly ADP-ribosylation proteins. Functional validation of several upregulated anti-viral innate immune proteins, including an active cGAS–STING axis, was performed. Through siRNA depletion, we describe a crucial role of the DNA sensor cGAS in restricting JEV replication. Further, many interferon-stimulated genes (ISGs) were observed to be induced in infected cells. We also observed activation of TLR2 and inhibition of TLR2 signalling using TLR1/2 inhibitor CU-CPT22-blocked production of inflammatory cytokines IL6 and TNF-α from virus-infected N9 microglial cells. The major proteins that were downregulated by infection were involved in cell adhesion (collagens), transport (solute carrier and ATP-binding cassette transporters), sterol and lipid biosynthesis. Several collagens were found to be transcriptionally downregulated in infected MEFs and mouse brain. Collectively, our data provide a bird’s-eye view into how fibroblast protein composition is rewired following JEV infection.

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Section: Discussionmentioning

confidence: 55%

Proteomic landscape of Japanese encephalitis virus-infected fibroblasts

Sharma

Chhabra

Aggarwal

et al. 2021

Journal of General Virology

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“…Most importantly, we also found that signaling related to immune response, Fcepsilon receptor signaling pathway, viral process, and symbiosis, encompassing mutualism through parasitism signaling affected by the COVID-19 targeting human cellular miRNAs (Table.6). The signaling mentioned above is important to fight against viral infections [45][46][47][48]. Both KEGG and GO pathway analysis revealed that COVID-19 targeting human cellular miRNAs are involved in the number of age-related complications.…”

Section: And Supplementarymentioning

confidence: 99%

COVID-19 Virulence in Aged Patients Might Be Impacted by the Host Cellular MicroRNAs Abundance/Profile

Fulzele¹,

Sahay²,

Yusufu³

et al. 2020

Aging and disease

111

150

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The World health organization (WHO) declared Coronavirus disease 2019 (COVID-19) a global pandemic and a severe public health crisis. Drastic measures to combat COVID-19 are warranted due to its contagiousness and higher mortality rates, specifically in the aged patient population. At the current stage, due to the lack of effective treatment strategies for COVID-19 innovative approaches need to be considered. It is well known that host cellular miRNAs can directly target both viral 3'UTR a nd coding region of the viral genome to induce the antiviral effect. In this study, we did in silico analysis of human miRNAs targeting SARS (4 isolates) and COVID-19 (29 recent isolates from different regions) genome and correlated our findings with aging and underlying conditions. We found 848 common miRNAs targeting the SARS genome and 873 common micr oRNAs targeting the COVID-19 genome. Out of a total of 848 miRNAs from SARS, only 558 commonly present in all COVID-19 isolates. Interestingly, 315 miRNAs are unique for COVID-19 isolates and 290 miRNAs unique to SARS. We also noted that out of 29 COVID-19 isolates, 19 isolates have identical miRNA targets. The COVID-19 isolates, Netherland (EPI_ISL_422601), Australia (EPI_ISL_413214), and Wuhan (EPI_ISL_403931) showed six, four, and four unique miRNAs targets, respectively. Furthermore, GO, and KEGG pathwa y analysis showed that COVID-19 targeting human miRNAs involved in various age-related signaling and diseases. Recent studies also suggested that some of the human miRNAs targeting COVID-19 decreased with aging and underlying conditions. GO and KEGG identified impaired signaling pathway may be due to low abundance miRNA which might be one of the contributing factors for the increasing severity and mortality in aged individuals and with other underlying conditions. Further, in vitro and in vivo studies are needed to validate some of these targets and identify potential therapeutic targets.

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“…The two most significant aquaculture species in East Asia, including South Korea, Japan, and China, are olive flounder (Paralichths olivaceus) and black rockfish (Sebastes schleglii), also known as Korean rockfish [17][18][19][20]. The production of olive flounder was 41,800 tons, while the output of black rockfish was 17,500 tons in 2021, according to the Korean Statistical Information Service data [21].…”

Section: Introductionmentioning

confidence: 99%

The residue of salinomycin in the muscles of olive flounder (Paralichthys olivaceus) and black rockfish (Sebastes Schlegeli) after oral administration analyzed by LC-Tandem-MS

Lee,

Woo,

Kim

et al. 2024

BMC Vet Res

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Background Salinomycin, an antibiotic, have potential as a veterinary drug for fish due to its anti-parasitic activity against several fish parasites. Thus the residual levels of salinomycin in muscles of two significant aquaculture species in Korea, olive flounder and black rockfish, were analyzed using HPLC-MS-MS. Results The proper method to analyze the residual salinomycin in fish muscles using LC-MS-MS was settled and the method was validated according to CODEX guidelines. The residues in three distinct groups for two fish species were analyzed using the matrix match calibration curves at points of five different times following oral administration. After oral administration, salinomycin rapidly breaks down in both olive flounder and black rockfish. After 7th days, the average residue in all groups of two fish spp. decreased below limit of quantitation (LOQ). Conclusion Due to low residue levels in fish muscles, salinomycin may therefore be a treatment that is safe for both fish and humans. This result could contribute to establishment of MRL (minimal residual limit) for approval of salinomycin for use in aquaculture.

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Gene expression profiles alteration after infection of virus, bacteria, and parasite in the Olive flounder (Paralichthys olivaceus)

Cited by 12 publications

References 77 publications

Proteomic landscape of Japanese encephalitis virus-infected fibroblasts

Proteomic landscape of Japanese encephalitis virus-infected fibroblasts

COVID-19 Virulence in Aged Patients Might Be Impacted by the Host Cellular MicroRNAs Abundance/Profile

The residue of salinomycin in the muscles of olive flounder (Paralichthys olivaceus) and black rockfish (Sebastes Schlegeli) after oral administration analyzed by LC-Tandem-MS

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