Lectin Histochemistry

Ponder, Bruce A.J.

doi:10.1016/b978-0-7236-0669-7.50015-5

Cited by 21 publications

(14 citation statements)

References 25 publications

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“…(2) Studies on the fate of labelled phago somes have shown that once taken up into the basal cells they can be later found in the columnar, mucous, enteroendocrine and Pa neth cells [9], indicating the omnipotent na ture of stem cells, which is confirmed by the clonal nature of chimeric crypts [14]. (3) The plateau value of the labelling in dex curve is 60% which is the same as the theoretical labelling index, that is the index expected if all the cells were in the cell divi sion cycle.…”

Section: Small Intestinementioning

confidence: 73%

Gastrointestinal Epithelial Cell Proliferation

Goodlad¹

1989

Dig Dis

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Section: Small Intestinementioning

confidence: 73%

Gastrointestinal Epithelial Cell Proliferation

Goodlad¹

1989

Dig Dis

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“…They included the following samples: meno pausal (3), proliferative (6), secretory (15), menstrual (2), and early gestational (4). Secretory endometrium was further subdivided into postovulatory day (POD) groups on the basis of established histological criteria 17].…”

Section: Methodsmentioning

confidence: 99%

“…Lectins are sugar-binding proteins or gly coproteins of nonimmune origin [1], They may be labelled with fluorescein or peroxi dase and localized in tissue sections [2], The lectins are especially useful for the study of the fast-growing tissues by virtue of their ability to bind to terminal carbohydrates that play an important role in cellular differentia tion [3], Though human endometrium is prone to undergo fast structural and physio logical changes, there are only a few reports dealing with its ability to bind lectins [4,5], In the course of our studies of lectin binding to cervical mucosa [6], we have found that the stromal cells in a biopsy specimen from a pregnant woman display a peculiarly high affinity to certain lectins that prompted us to start a current investigation.…”

Section: Introductionmentioning

confidence: 99%

Lectin Binding to Normal Human Endometrium

Bychkov¹,

Toto²

1986

Gynecol Obstet Invest

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Avidin-biotin-peroxidase labelling technique was used to localize the binding sites of peanut agglutinin (PNA), Ulexeuropaeus (UEA-I), wheat germ agglutinin (WGA), concanavalin A (Con A), and Ricinuscommunis (RCA-I) in 30 samples of endometrium at different stages of the menstrual cycle and in early pregnancy. PNA and UEA-I exhibit weak binding to the apical portions of epithelial cells in proliferative and secretory phases, but become strongly positive in pregnancy. WGA and RCA-I show weak binding to glandular epithelium in the proliferative phase, but increase their affinity in the secretory phase. Con A demonstrates strong affinity to decidual cells

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“…One of the key roles in cellular differentiation belongs to the glucoconjugates [1][2][3] that may be assessed by the binding of specific lectins to the oligosaccharides in histo logic sections using such labels as fluorescein isothyocyanate, peroxidase or avidin-biotin-peroxidase complex [4], Thus, lectin histochemistry opens new horizons for the study of tissue carbohydrates in normal and pathological conditions [5][6][7],…”

Section: Introductionmentioning

confidence: 99%