Lectin histochemical studies on the olfactory gland and two types of gland in vomeronasal organ of the brown bear

Tomiyasu, Jumpei; Kondoh, Daisuke; Sakamoto, Hideyuki; Matsumoto, Naoya; Haneda, Shingo; Matsui, Motozumi

doi:10.1016/j.acthis.2018.07.003

Cited by 8 publications

(8 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast, Kondoh et al (2020) reported that vomeronasal glands in dogs were solely PAS-positive. Regarding other Carnivore species, Tomiyasu et al (2018) found both PAS- and AB-positive glands in bears, while in cats and dogs, only PAS-positive glands were identified by Salazar et al (1996) and Kondoh et al (2020), respectively. These differences may be attributed to the specific region of the VNO examined, as most studies have focused only on its central region where we have found the glandular tissue density to be much lower.…”

Section: Discussionmentioning

confidence: 95%

Sensory Adaptations: Insights into the Vomeronasal System of the Iberian Wolf

Ortiz-Leal,

Torres,

Barreiro-Vázquez

et al. 2023

Preprint

View full text Add to dashboard Cite

Wolves, like other canids, extensively use chemical signals for various aspects of communication, including territory maintenance, reproductive synchronization, and social hierarchy signaling. Pheromone-mediated chemical communication operates unconsciously among individuals, acting as a mysterious sixth sense that regulates both their physiology and behavior. Despite their crucial role in the life of the wolf, there is a surprising lack of comprehensive research on the neuroanatomical and physiological bases of chemical communication in wolves. This study delves into the Iberian wolf vomeronasal system (VNS) and examines potential changes brought about by dog domestication. Our findings show that the Iberian wolf possesses a fully functional VNS vital for pheromone-mediated communication. While macroscopic similarities between the wolf and domestic dog VNS are observed, there are notable microscopic differences. These include the presence of neuronal clusters associated with the sensory epithelium of the vomeronasal organ (VNO) and a higher differentiation degree of the accessory olfactory bulb (AOB). Immunohistochemical markers reveal the expression of the two main families of vomeronasal receptors (V1R and V2R) in the VNO. However, only the V1R family is expressed in the AOB. These findings not only provide deep insights into the VNS of the wolf but also hint at how domestication might have altered neural configurations that underpin species-specific behaviors. This understanding has implications for innovative strategies, such as employing semiochemicals for wolf population management, aligning with modern conservation goals.

show abstract

Section: Discussionmentioning

confidence: 95%

Sensory Adaptations: Insights into the Vomeronasal System of the Iberian Wolf

Ortiz-Leal,

Torres,

Barreiro-Vázquez

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…LEA (Vector‐B1175), which comes from tomato, Lycopersicon esculentum , and recognizes N‐acetyl‐glucosamine (Salazar, Lombardero, Cifuentes, et al, 2003). VVA, which is isolated from fodder vetch, Vicia villosa , binds specifically to N‐acetylgalactosamine structures (Tomiyasu et al, 2018). SBA (Vector‐B1235), which comes from fodder soybean, Glycine max , binds preferentially to oligosaccharide structures with N‐acetylgalactosamine and to galactose residues (Park et al, 2012).…”

Section: Methodsmentioning

confidence: 99%

“…3. VVA, which is isolated from fodder vetch, Vicia villosa, binds specifically to N-acetylgalactosamine structures (Tomiyasu et al, 2018).…”

Section: Lectin Histochemical Stainingmentioning

confidence: 99%

Immunohistological study of the unexplored vomeronasal organ of an endangered mammal, the dama gazelle (Nanger dama)

Torres

Ortiz‐Leal

Ferreiro³

et al. 2023

Microscopy Res & Technique

View full text Add to dashboard Cite

Dama gazelle is a threatened and rarely studied species found primarily in northern Africa. Human pressure has depleted the dama gazelle population from tens of thousands to a few hundred individuals. Since 1970, a founder population consisting of the last 17 surviving individuals in Western Sahara has been maintained in captivity, reproducing naturally. In preparation for the future implementation of assisted reproductive technology, certain aspects of dama gazelle reproductive biology have been established. However, the role played by semiochemical‐mediated communications in the sexual behavior of dama gazelle remains unknown due partially to a lack of a neuroanatomical or morphofunctional characterization of the dama gazelle vomeronasal organ (VNO), which is the sensory organ responsible for pheromone processing. The present study characterized the dama gazelle VNO, which appears fully equipped to perform neurosensory functions, contributing to current understanding of interspecies VNO variability among ruminants. By employing histological, lectin‐histochemical, and immunohistochemical techniques, we conducted a detailed morphofunctional evaluation of the dama gazelle VNO along its entire longitudinal axis. Our findings of significant structural and neurochemical transformation along the entire VNO suggest that future studies of the VNO should take a similar approach. The present study contributes to current understanding of dama gazelle VNO, providing a basis for future studies of semiochemical‐mediated communications and reproductive management in this species.Research Highlights This exhaustive immunohistological study of the vomeronasal organ (VNO) of the dama gazelle provides the first evidence of notable differences in the expression of neuronal markers along the rostrocaudal axis of the VNO. This provides a morphological basis for the implementation of pheromones in captive populations of dama gazelle.

show abstract

“…For this reason, we have used the following five lectins in our research: UEA (Vector-L1060), which comes from gorse, Ulex europaeus , and marks the L- fucose pathway (Kondoh et al, 2018). LEA (Vector-B1175), which comes from tomato, Lycopersicon esculentum , and recognizes N-acetyl-glucosamine (Salazar & Sanchez-Quinteiro, 2003). VVA, which is isolated from fodder vetch, Vicia villosa , binds specifically to N- acetylgalactosamine structures (Tomiyasu et al, 2018). SBA (Vector-B1235), which comes from fodder soybean, Glycine max , binds preferentially to oligosaccharide structures with N-acetylgalactosamine and to galactose residues (Park et al, 2012).…”

Section: Methodsmentioning

confidence: 99%

“…VVA, which is isolated from fodder vetch, Vicia villosa , binds specifically to N- acetylgalactosamine structures (Tomiyasu et al, 2018).…”

Section: Methodsmentioning

confidence: 99%

Exploring the vomeronasal organ in an endangered antelope species

Torres¹,

Ortiz‐Leal²,

Ferreiro³

et al. 2023

Preprint

View full text Add to dashboard Cite

The dama gazelle is a threatened and scarcely studied species of northern Africa. Human pressure has depleted the population of dama gazelles from tens of thousands to a few hundred individuals. Since 1970, after deriving from a founder population of the last 17 surviving individuals in the Western Sahara, it has been reproduced naturally in captivity. Although certain aspects of the reproductive biology of the dama gazelle have been established in preparation for the future implementation of assisted reproductive technology there is a lack of information regarding the role of semiochemical-mediated communication in the sexual behavior of the dama gazelle. This is partially due to the lack of a neuroanatomical and morphofunctional characterization of the dama gazelle vomeronasal organ (VNO); the sensory organ responsible for the processing of pheromones. This study aims to determine the presence in the dama gazelle of a VNO fully equipped to carry out its neurosensory function and to contribute to the understanding of the interspecific variability of the VNO of ruminants. Employing histological, lectin-histochemical and immunohistochemical techniques we have performed a detailed morphofunctional evaluation of the dama gazelle VNO along its entire longitudinal axis. The findings suggest that studies of the VNO should take a similar approach, as there are significant structural and neurochemical transformations that the organ exhibits as a whole. This study contributes to the understanding of the VNO in dama gazelles and provides a basis for future studies on semiochemical-mediated communication and reproductive management of this species.

show abstract

Lectin histochemical studies on the olfactory gland and two types of gland in vomeronasal organ of the brown bear

Cited by 8 publications

References 25 publications

Sensory Adaptations: Insights into the Vomeronasal System of the Iberian Wolf

Sensory Adaptations: Insights into the Vomeronasal System of the Iberian Wolf

Immunohistological study of the unexplored vomeronasal organ of an endangered mammal, the dama gazelle (Nanger dama)

Exploring the vomeronasal organ in an endangered antelope species

Contact Info

Product

Resources

About