Lectin-binding histochemistry of verruca vulgaris and seborrheic keratosis.

Abstract

Sugar residues in the epithelial cells of verruca vulgaris and seborrheic keratosis were demonstrated by the horseradish peroxidase-conjugated lectin methods. The lectins, Con A, RCA-1, UEA-1, DBA, PNA, SBA, and WGA were used to bind specifically to the sugars, Man, Glc, Gal, Fuc, Ga1NAc, G1cNAc and NANA. The cytochemical distribution of lectin bindings were limited to the cell surface, intercellular materials and basement membrane in the epithelial structures of the lesions. The lectin bindings in the basal layer cells were negative or weak, but increased gradually in upper spinous cells from moderate to strong levels. Vacuolated cells in verruca vulgaris showed positive lectin stainings. The intercellular materials of parakeratinized cells bound lectins differently, but orthokeratinized cells failed to bind lectins. The regional distribution of lectin bindings in stratified squamous epithelia may indicate an expression of keratinocytic stratification as well as in keratinized benign lesions.

Histochemical observation of lectin-binding patterns in Paget's disease.

“…RCA-1: Paget cells displayed very weak staining, and squamous cells slight to Identification of sugar residues in cellular macromolecules was applied with use of the lectin binding site as a specific marker for detecting sugar residues (9,23,26). Recently, lectin binding sites or binding affinities in varying types: of the skin and mucosal lesions have been described as well as in normal squamous cell epithelia (3,7,11,(15)(16)(17)(18)24) . In malignantly transformed epithelia, lectin binding patterns displayed a change to irregular distribution with reduced stainability (15)(16)(17).…”

“…Stratified squamous epithelia, such as the skin and mucosa, have indicated a regular distribution of individual layers in each lectin binding pattern, and those in malignant lesions indicated an irregular distribution (3,7,11,(15)(16)(17)(18)24). Recently the distribution of PNA and WGA binding in Paget's disease was carried out and was compared to that in normal skin elements (28).…”

Histochemical observations of lectin-binding sites and keratin distribution in calcifying epithelioma of Malherbe.

“…However, no detailed report has been made concerning the immunohistochemical detection of keratin peptides in calcifying epithelioma. Recently, lectin-binding sites which represent specific sugar residues have been shown to occur in hyperkeratinized epithelial lesions of premalignant and malignant tumors (3,4,(9)(10)(11)(12). The present study was undertaken to show the distribution of both keratin and sugar residues in calcifying epithelioma, particularly in tumor cells with higher keratinization tendency.…”