Lecithin-based nanoemulsions of traditional herbal wound healing agents and their effect on human skin cells

Vater, Claudia; Bosch, Leonie; Mitter, A.; Göls, Thomas; Seiser, Saskia; Heiß, Elke H.; Elbe‐Bürger, Adelheid; Wirth, Michael; Valenta, Claudia; Klang, Victoria

doi:10.1016/j.ejpb.2021.11.004

Cited by 21 publications

(10 citation statements)

References 51 publications

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“…Surfactants (including polysorbates) and components of the oil phase of emulsified nanocarriers have been demonstrated to affect membrane permeability and increase the release of inflammatory cytokines, and thus, impact cell viability [39][40][41][42]. This is consistent with the previously reported ability of nano and microemulsions to affect the viability of tumor and non-tumor cells depending on the type/concentration of the oil phase and surfactants [43][44][45][46], and might contribute to the overall drug-loaded nanoemulsion cytotoxicity. Presence of HA did not affect the viability of cells in a pronounced manner (Figure 2 and Table 2).…”

Section: Influence Of Nanoemulsion Composition On Its Cytotoxicitysupporting

confidence: 89%

Phosphatidylcholine-Based Nanoemulsions for Paclitaxel and a P-Glycoprotein Inhibitor Delivery and Breast Cancer Intraductal Treatment

Salata

Lopes

2022

Pharmaceuticals

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In this study, incorporation of the cytotoxic agent paclitaxel and the P-glycoprotein inhibitor elacridar in hyaluronic acid (HA)-modified nanoemulsions was studied for intraductal delivery and breast cancer localized treatment. To improve cytotoxicity, we investigated the incorporation of perillyl alcohol or tributyrin as components of the nanoemulsion oil phase. The nanoemulsions presented size <180 nm and negative zeta potential. Both tributyrin and perillyl alcohol increased nanoemulsion cytotoxicity in MCF-7 cells, but not in MDA-MB-231. However, perillyl alcohol reduced nanoemulsion stability in the presence of the drugs. Concomitant incorporation of paclitaxel and elacridar in HA- and tributyrin-containing nanoemulsions (PE-NETri) increased cytotoxicity and reduced IC50 by 1.6 to 3-fold in MCF-7 and MDA-MB-231 cells compared to the nanoemulsion containing only paclitaxel (P-NE). This nanoemulsion also produced a 3.3-fold reduction in the viability of MDA-MB-231 spheroids. Elacridar incorporated in the nanoemulsion was capable of inhibiting P-glycoprotein in membranes. In vivo intraductal administration of the NE containing HA resulted in a three-fold higher retention of a fluorescent marker compared to a solution or nanoemulsion without HA, demonstrating the importance of HA. The nanoemulsion produced no histological changes in the mammary tissue. These results support the potential applicability of the nanoemulsion for local breast cancer management.

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Section: Influence Of Nanoemulsion Composition On Its Cytotoxicitysupporting

confidence: 89%

Phosphatidylcholine-Based Nanoemulsions for Paclitaxel and a P-Glycoprotein Inhibitor Delivery and Breast Cancer Intraductal Treatment

Salata

Lopes

2022

Pharmaceuticals

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“…Such structures are developed when cells are stimulated to migrate until they come into contact with each other and accelerate re-epithelialization. In the last stage, betulin stimulates various differentiation markers, such as involucrin, keratin 10, and transglutaminase, which differentiates cells and helps in the maturation and remodeling of wounds ( Vater et al, 2022 ).…”

Section: Semmentioning

confidence: 99%

“…In these, authors reported upregulation of various pro-inflammatory cytokines, chemokines, and cyclooxygenase 2 on gene and protein levels ( Ebeling et al, 2014 ). In another study, birch bark extract was loaded into a lecithin-based nanoemulsion that showed good results in scratch assays ( Vater et al, 2022 ). Oleogel-S10 loaded with 10% triterpene dry extract from the bark of Betulae cortex showed faster re-epithelialization of wounds in patients with dystrophic epidermolysis bullosa ( Schwieger-Briel et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Design and evaluation of sodium alginate-based hydrogel dressings containing Betula utilis extract for cutaneous wound healing

Ishfaq

Khan

Khalid

et al. 2023

Front. Bioeng. Biotechnol.

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Traditional wound dressings have a limited capacity to absorb exudates, are permeable to microbes, and may adhere to wounds, which leads to secondary injuries. Hydrogels are promising alternative dressings to overcome the above challenges. In this study, we developed sodium alginate-based hydrogel films loaded with Betula utilis bark extract. These films were prepared via solvent-casting crosslinking method and evaluated for wound healing activity. Prepared films were 0.05–0.083 mm thick, flexible with folding endurance ranging from 197–203 folds, which indicates good physical properties. Optimized formulations exhibited successful loading of extract in the film matrix without any interaction as confirmed by FTIR. Maximum zone of inhibition against Gram-positive and Gram-negative bacteria was achieved by optimum formulation (B6), i.e., 19 mm and 9 mm, respectively, with > 90% scavenging activity. Furthermore, this optimum formulation (B6) was able to achieve 93% wound contraction in rats. Histograms of the optimized formulation treated group also revealed complete reepithelization of wounds. Conclusively, our extract-loaded hydrogel dressing successfully demonstrated its potential for cutaneous wound healing.

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“…The findings of the study by Faraji et al 16 showed that BC can be used as a safe natural medicine in the healing of episiotomy wounds. Today, various studies have been performed to evaluate the different chemical and plant compounds as nanoemulsion in wound healing, which have yielded significant results 55,56 . The result of this study also provided a new horizon for the use of BC plant as nanoparticles for wound healing in future studies.…”

Section: Discussionmentioning

confidence: 75%

Evaluation of antibacterial and wound‐healing activities of alcoholic extract of Boswellia carterii, in vitro and in vivo study

Hasson

Jasim

Salman

et al. 2022

J of Cosmetic Dermatology

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Wounds are defined as the cellular and anatomical destruction of tissues that can be caused by chemical, physical, microbiological, thermal, or immunological damage, leading to skin rupture. 1 Wound healing is one of the most complex biological processes, with four overlapping phases: hemostasis, inflammation, proliferative, and maturation. These phases lead to the regeneration of new tissue. In burn injuries, blood coagulation occurs due to damaged capillaries, followed by inflammation. 2,3 The global cost of wound care averaged 2.8 billion dollars in 2014 and is expected to reach 3.5 billion dollars by 2021. 4 A 2018 analysis

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Lecithin-based nanoemulsions of traditional herbal wound healing agents and their effect on human skin cells

Cited by 21 publications

References 51 publications

Phosphatidylcholine-Based Nanoemulsions for Paclitaxel and a P-Glycoprotein Inhibitor Delivery and Breast Cancer Intraductal Treatment

Phosphatidylcholine-Based Nanoemulsions for Paclitaxel and a P-Glycoprotein Inhibitor Delivery and Breast Cancer Intraductal Treatment

Design and evaluation of sodium alginate-based hydrogel dressings containing Betula utilis extract for cutaneous wound healing

Evaluation of antibacterial and wound‐healing activities of alcoholic extract of Boswellia carterii, in vitro and in vivo study

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