Lebectin and lebecetin, two C-type lectins from snake venom, inhibit α5β1 and αv-containing integrins

Sarray, Sameh; Delamarre, Estelle; Marvaldi, Jacques; Ayeb, Mohamed El; Marrakchi, Naziha; Luis, José

doi:10.1016/j.matbio.2007.01.001

Cited by 55 publications

(41 citation statements)

References 46 publications

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“…34 However, in the last years increasing evidences show that some CLP can also inhibit integrin-dependent function. 11,12,35 Here, we extend this observation to another class of snake venom proteins, the group IIA PLA 2 s.…”

Section: Discussionsupporting

confidence: 55%

“…They inhibit cell adhesion, migration, proliferation and angiogenesis by interacting with integrins. [9][10][11][12] Phospholipases A2 (PLA 2 , EC 3.1.1.4) are generally Ca 2 þ -dependent enzymes that catalyze the hydrolysis of the sn-2 fatty acyl bond of glycerophospholipids. Secreted PLA 2 s are small proteins (14-18 kDa) usually containing 5-8 disulfide bonds and possessing a His/Asp dyad required for catalysis.…”

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confidence: 99%

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CC-PLA2-1 and CC-PLA2-2, two Cerastes cerastes venom-derived phospholipases A2, inhibit angiogenesis both in vitro and in vivo

Kessentini-Zouari

Jebali

Taboubi

et al. 2010

Laboratory Investigation

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Integrins are essential in the complex multistep process of angiogenesis and are thus attractive targets for the development of antiangiogenic therapies. Integrins are antagonized by disintegrins and C-type lectin-like proteins, two protein families from snake venom. Here, we report that CC-PLA2-1 and CC-PLA2-2, two novel secreted phospholipases A 2 (PLA 2 ) isolated from Cerastes cerastes venom, also showed anti-integrin activity. Indeed, both PLA 2 s efficiently inhibited human brain microvascular endothelial cell adhesion and migration to fibrinogen and fibronectin in a dose-dependent manner. Interestingly, we show that this anti-adhesive effect was mediated by a5b1 and av-containing integrins. CC-PLA2s also impaired in vitro human brain microvascular endothelial cell tubulogenesis on Matrigel and showed antiangiogenic activity in vivo in chicken chorioallantoic membrane assay. The complete PLA 2 cDNAs were cloned from a venom gland cDNA library. Mature CC-PLA2-1 and CC-PLA2-2 contain 121 and 120 amino acids, respectively, including 14 cysteines each and showed 83% identity. Tertiary model structures of CC-PLA2-1 and CC-PLA2-2 were generated by homology modeling. This is thus the first study describing an antiangiogenic effect for snake venom PLA 2 s and reporting first clues to their mechanism of action on endothelial cells.

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Section: Discussionsupporting

confidence: 55%

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confidence: 99%

CC-PLA2-1 and CC-PLA2-2, two Cerastes cerastes venom-derived phospholipases A2, inhibit angiogenesis both in vitro and in vivo

Kessentini-Zouari

Jebali

Taboubi

et al. 2010

Laboratory Investigation

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“…Proteins including disintegrins, serine proteinase, metalloproteinase, CLP, PLA 2 and LAAO have been purified and identified in the venom of different subspecies of M. lebetina (Marcinkiewicz et al 2003;Tõnismägi et al 2006;Sarray et al 2007;Park et al 2012;Samel et al 2012Samel et al , 2013. Sanz et al (2008) identified PLA 2 , LAAO, metalloproteinase, serine proteinase, natriuretic peptide, disintegrin, BPP and CRISP protein families in the venom of M. l. obtusa from Armenia by a mass spectrometry-based proteomics approach.…”

Section: Discussionmentioning

confidence: 99%

Screening of cytotoxic and antimicrobial activity potential of AnatolianMacrovipera lebetina obtusa(Ophidia: Viperidae) crude venom

Özen

İğci

Yalçın

et al. 2015

Frontiers in Life Science

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The effects of snake venoms have been well known since ancient times. They contain a variety of biologically active proteins which have therapeutic potential. This study investigated the cytotoxic and antimicrobial activities of Anatolian Macrovipera lebetina obtusa venom against various cancer cells, Gram-negative and Gram-positive bacteria, and a fungal species. A549, HeLa, CaCo-2, U-87 MG and MCF-7 cancer cell lines and a normal cell line (Vero) were screened by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The antimicrobial activity was evaluated by determining the minimum inhibitory concentration (MIC) using the broth dilution method. The species included were Escherichia coli ATCC 25922, E. coli 0157:H7, Enterococcus faecalis, Enterococcus faecium DSM 13590, Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis ATCC 12228, Salmonella typhimurium CCM 5445, Proteus vulgaris ATCC 6957, Bacillus cereus ATCC 7064 and Candida albicans ATCC 10239. Half-maximal inhibitory concentration (IC 50 ) values of M. l. obtusa venom on cultured cells varied from 1.18 ± 0.11 to 12.80 ± 0.22 μg/ml, with the most potent activities against Vero, U-87 MG, MCF-7 and CaCo-2 cells. Venom showed moderate antifungal activity against C. albicans, with an MIC of 62.50 μg/ml. In short, the venom of Anatolian M. l. obtusa showed promising results as a potential source of alternative therapeutics, cytotoxic and antifungal agent prototypes.

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“…Cells were washed and then fixed by 1 glutaraldehyde. After staining by 0.1 crystal violet, cells were lysed with 1 SDS and the optical density was measured at 600 nm by a microplate reader 15 .…”

Section: Proliferation Assaymentioning

confidence: 99%

Chemical Composition and Biological Activities of <i>Allium roseum</i> L. var. grandiflorum Briq. Essential Oil

et al. 2015

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International audienceAllium roseum L. (Alliaceae) endemic mediterranean specie was represented in the North Africa by 12 different taxa. In the present study, chemical composition, antiproliferative, antioxidant and antimicrobial activities of the essential oil extracted from A. roseum var. grandiflorum Briq. bulbs collected in the North of Tunisia were investigated. Chemical characterization has shown methyl methanethiosulfinate as major sulphurous compounds. A. roseum bulbs essential oil provides interesting antiproliferative activity against two human colonic adenocarcinoma HT29 and CACO2 cell lines in dose-dependent manner with a half-maximal inhibition (IC50) of 4.64 µg/mL and 8.22 µg/mL respectively. The antioxidant activity, as determined by FRAP assay, was 285 µmol equivalent Trolox/g of essential oil. The scavenging effect on DPPH radicals of essential oil was estimated as IC50 values at 156 µg/mL. The inhibition of superoxide anion production in a model of cancer cell lines was significant for both lines HT29 and CACO2 with IC50 of 20.25 µg/mL and 29.12 µg/mL respectively. Allium roseum essential oil exhibited antibacterial and antifungal activities with a high effectiveness against Candida albicans given by an MIC value of 0.019 mg/mL. This biological effect appears to be related mainly to the presence of organosulfur compounds

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Lebectin and lebecetin, two C-type lectins from snake venom, inhibit α5β1 and αv-containing integrins

Cited by 55 publications

References 46 publications

CC-PLA2-1 and CC-PLA2-2, two Cerastes cerastes venom-derived phospholipases A2, inhibit angiogenesis both in vitro and in vivo

CC-PLA2-1 and CC-PLA2-2, two Cerastes cerastes venom-derived phospholipases A2, inhibit angiogenesis both in vitro and in vivo

Screening of cytotoxic and antimicrobial activity potential of AnatolianMacrovipera lebetina obtusa(Ophidia: Viperidae) crude venom

Chemical Composition and Biological Activities of <i>Allium roseum</i> L. var. grandiflorum Briq. Essential Oil

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