The three-helix theory provides a new theoretical basis for the breakthrough construction of the hybrid teaching system. This paper innovatively analyzes from the perspective of multiple subjects and attempts to provide a theoretical basis and practical suggestions for the construction of a hybrid teaching system. Firstly, this paper combs the development process of the hybrid teaching system and defines the hybrid teaching system from the perspective of multiple subjects. Secondly, the article proposes a four-party co-construction mechanism of the hybrid teaching system, forming a coordinated guarantee mechanism for the participation of schools, enterprises, governments and social organizations. It constructs a hybrid teaching system across schools, multi-subjects and the whole process. Finally, the article rationally points out that the participation of multiple subjects in the construction of a mixed teaching system may have difficulties in enthusiasm, participation and coordination. This system is of great significance for enriching the theory and practice of hybrid teaching. It has great room for promotion in the context of the knowledge society, wisdom education and multi-subject governance, but its difficulties need to be overcome, and the practical results need to be further verified.