Learning about PRP using cell-based models

Beitzel, Knut; McCarthy, Mary Beth; Russell, Ryan P.; Apostolakos, John; Cote, Mark P.; Mazzocca, Augustus D.

doi:10.11138/mltj/2014.4.1.038

Cited by 6 publications

(4 citation statements)

References 42 publications

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“…Cell culture models enable evaluating the effects of different PRP products under controlled and standardized conditions. 4 When the standardized PRP powder is used, the nonexistent variability among different PRP preparations might lead to more reliable results in clinical application. 20 A recent in vitro study showed that the effects of PRP on mesenchymal stem cells depend on platelet count.…”

Section: Discussionmentioning

confidence: 99%

Dose-Dependent Effects of Platelet-Rich Plasma Powder on Chondrocytes In Vitro

et al. 2020

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Background: Platelet-rich plasma (PRP) is widely used in sports medicine. However, neither preparation nor parameters for clinical application, such as concentration, timing, and number of applications, are standardized, making research and clinical utilization challenging. Purpose: To investigate the effect of varying doses of PRP powder in terms of different concentrations, timing, and number of applications on human chondrocytes in a reproducible cell culture model. Study Design: Controlled laboratory study. Methods: A standardized lyophilized platelet growth factor preparation (PRP powder) was used to stimulate human chondrocytes. Chondrocytes were cultivated for 2 weeks with different stimulation frequencies (2×, 3×, 6×) and different concentrations of PRP powders (0.5%, 1%, 5%). Cell proliferation and metabolic cell activity were analyzed on days 7 and 14. Phenotypic changes were visualized through live-dead staining. Chondrogenic differentiation was quantified with enzyme-linked immunosorbent assay to assess the synthesis of procollagen types 1 and 2. Furthermore, sulfated proteoglycans and glycosaminoglycans were analyzed. Results: Human chondrocytes exhibited a significant dose- and time-dependent increase after 14 days in cell number (1% and 5% PRP powder vs unstimulated control: 7.95- and 15.45-fold increase, respectively; 2× vs 6× stimulation with 5% PRP powder: 4.00-fold increase) and metabolic cell activity (1% and 5% PRP powder vs unstimulated control: 3.27-fold and 3.58-fold change, respectively). Furthermore, cells revealed a significant increase in the amount of bone-specific procollagen type 1 (14 days, 1.94-fold) and sulfated glycosaminoglycans (14 days, 2.69-fold); however, no significant change was observed in the amount of cartilage-specific collagen type 2. Conclusion: We showed that chondrocytes exhibit a significant dose- and time-dependent increase in cell number and metabolic cell activity. The standardized use of growth factor concentrates in cell culture models can contribute to clinical knowledge in terms of dosage and timing of PRP applications. Clinical Relevance: Problems with PRP, such as the absence of standardization, lack of consistency among studies, and unknown dosage, could be solved by using characterized PRP powder made by pooling and lyophilizing multiple platelet concentrates. The innovative PRP powder generates new possibilities for PRP research, as well as for the treatment of patients.

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Section: Discussionmentioning

confidence: 99%

Dose-Dependent Effects of Platelet-Rich Plasma Powder on Chondrocytes In Vitro

et al. 2020

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“…Therefore, the final PRP is ultimately determined by the nature and characteristics of the patient's blood at the time of the draw [28].…”

Section: Discussionmentioning

confidence: 99%

Long-term beneficial effects of platelet-rich plasma for non-insertional Achilles tendinopathy

Guelfi

Pantalone

Vanni

et al. 2015

Foot and Ankle Surgery

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“…To date there are several commercially available PRP systems 9,15,55 , which differ among them in either the centrifugation method, platelet concentration, release rate of growth factors 9,56,57 , white 58,59 and red blood cell concentration and/or activation 60 . Differences among PRP commercial presentations have not been clarified, regarding the optimal concentration of growth factors delivery and their effects on tendon regeneration.…”

Section: Limitations Of Use Of Prpmentioning

confidence: 99%

Growth factor delivery vehicles for tendon injuries: Mesenchymal stem cells and Platelet Rich Plasma

Guevara-Álvarez

Schmitt

Russell

et al. 2014

MLTJ

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SummaryBackground: tendon tissue shows limited regeneration potential with formation of scar tissue and inferior mechanical properties. The capacity of several growth factors to improve the healing response and decrease scar formation is described in different preclinical studies. Besides the application of isolated growth factors, current research focuses on two further strategies to improve the healing response in tendon injuries: platelet rich plasma (PRP) and mesenchymal stem cells (MSCs). Objective: the present review focuses on these two options and describes their potential to improve tendon healing. Results: in vitro experiments and animal studies showed promising results for the use of PRP, however clinical controlled studies have shown a tendency of reduced pain related symptoms but no significant differences in overall clinical scores. On the other hand MSCs are not totally arrived in clinical use so that there is still a lack of randomized controlled trials. In basic research experiments they show an extraordinary paracrine activity, anti-inflammatory effect and the possibility to differentiate in tenocytes when different activating-factors are added. Conclusion: preclinical studies have shown promising results in improving tendon re-

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Learning about PRP using cell-based models

Cited by 6 publications

References 42 publications

Dose-Dependent Effects of Platelet-Rich Plasma Powder on Chondrocytes In Vitro

Dose-Dependent Effects of Platelet-Rich Plasma Powder on Chondrocytes In Vitro

Long-term beneficial effects of platelet-rich plasma for non-insertional Achilles tendinopathy

Growth factor delivery vehicles for tendon injuries: Mesenchymal stem cells and Platelet Rich Plasma

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