Learning about PRP using cell-based models

Abstract

Studies using in vitro cell models enable evaluation of the effects of different PRP products under very controlled and standardized conditions. Therefore the results of such studies build the basis for understanding the variable results of clinical studies on the use of PRPs. The main lessons learned through the use of in vitro cell models are that many different PRP products exist and researchers have to report on component variation within each product. These different products may have distinctive effects on the various cells treated in musculoskeletal injuries; therefore, some products might be more beneficial in certain indication than others. In its utilization in cell models, PRP may generate a variety of positive effects on cell proliferation, recovery, and inflammatory response. There might also be a benefit to adding PRP to current pharmacological therapies (e.g. corticosteroids) to prevent their commonly known negative effects on e.g. tendon and cartilage tissue.

Growth factor delivery vehicles for tendon injuries: Mesenchymal stem cells and Platelet Rich Plasma

“…To date there are several commercially available PRP systems 9,15,55 , which differ among them in either the centrifugation method, platelet concentration, release rate of growth factors 9,56,57 , white 58,59 and red blood cell concentration and/or activation 60 . Differences among PRP commercial presentations have not been clarified, regarding the optimal concentration of growth factors delivery and their effects on tendon regeneration.…”

Single Platelet-Rich Plasma Injection for Early Stage of Osteoarthritis of the Knee

“…In vitro studies showed that PRP, in particular leukocyte-rich plasma (L-PRP), is able to suppress inflammatory mediator concentration and gene expression in synovium and cartilage tissue, 9 and this could explain the pain improvement and promising results in some clinical series. Although there have been many clinical studies on the anti-inflammatory effects of corticosteroids and hyaluronic acid (HA) injections for knee OA, 10 clinical studies using PRP are limited.…”

Autologous platelet plasma: promising method in the osteoarthritis treatment

“…Tissue wound repair is a complex process in which a variety of cellular functions such as chemotaxis, angiogenesis, cell pro liferation, extracellular matrix formation and the «cleansing» macrophage coexist, sequentially and covertly [5,6]. These func tions form a complex in which three relatively distinct phases are classically distinguished: inflammation, proliferation and remod eling 3, 4, 5, 6, 7.…”

“…and the type of tissue (tendon, muscle, bone, etc.). PAP efficacy for the cells reparation process activation was demonstrated experimentally and confirmed in few clinical stud ies but researches used different PAP preparations so results often are not comparable [6]. Also platelet quantification and the growth factor content definition must be defined in order to understand molecular mechanisms behind PRP regenerative strength.…”

“…Also platelet quantification and the growth factor content definition must be defined in order to understand molecular mechanisms behind PRP regenerative strength. Standardization of PRP preparations is thus urgently needed [6][7][8].…”