Leaf Senescence: The Chloroplast Connection Comes of Age

Mayta, Martín Leonardo; Hajirezaei, Mohammad‐Reza; Carrillo, Néstor; Lodeyro, Anabella F.

doi:10.3390/plants8110495

Cited by 45 publications

(27 citation statements)

References 145 publications

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“…Overexpressing OsGLK1 in WT plants also led to abnormal plastid development and tapetum degradation. Plastids differentiate into elaioplasts during tapetum development, a process required for the phase transition 46,47 . Our findings suggest that the fluctuating expression of OsGLK1 is required for the differentiation of plastids into elaioplasts.…”

Section: Osppr2-1 Regulates Osglk1 Expression To Mediate Tapetum Devementioning

confidence: 99%

The rice cytosolic pentatricopeptide repeat protein OsPPR2-1 regulates OsGLK1 to control tapetal plastid development and programmed cell death

Zheng

Dong

et al. 2021

Preprint

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Most pentatricopeptide repeat (PPR) proteins localize to plastids or mitochondria, where they participate in RNA metabolism and post-transcriptionally regulate organelle gene expression. However, whether PPR proteins regulate the expression of nucleus-encoded genes remains unclear. Here, we uncovered a function for the rice (Oryza. sativa L.) PPR protein OsPPR2-1 (Os02g0110400) in pollen development and showed that, in contrast to most other PPR proteins, OPPR2-1 resides in the cytoplasm. Downregulating OsPPR2-1 expression led to abnormal plastid development in tapetal cells, prolonged programmed cell death (PCD), prolonged tapetum degradation, and significantly reduced pollen fertility. Transcriptome analysis revealed that the expression of OsGOLDEN-LIKE 1 (OsGLK1), encoding a transcription factor that regulates plastid development and maintenance, was significantly higher in plants with downregulated OsPPR2-1 expression compared to the wild type. Moreover, OsPPR2-1 bound to the OsGLK1 mRNA in RNA immunoprecipitation and RNA-electrophoretic mobility shift assays. An in vitro cleavage assay showed that OsPPR2-1 could degrade the OsGLK1 mRNA. Notably, knockdown of OsGLK1 partially restored pollen fertility in OsPPR2-1-knockdown plants and OsGLK1-overexpressing plants showed abnormal tapetum and plastid development, similar to the OsPPR2-1-knockdown plants. Together, our findings demonstrate that OsPPR2-1 regulates OsGLK1 expression, thereby controlling plastid development and PCD in the tapetum.

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Section: Osppr2-1 Regulates Osglk1 Expression To Mediate Tapetum Devementioning

confidence: 99%

The rice cytosolic pentatricopeptide repeat protein OsPPR2-1 regulates OsGLK1 to control tapetal plastid development and programmed cell death

Zheng

Dong

et al. 2021

Preprint

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“…By comparing scions from the juvenile and the adult phases of the same hybrid, we observed that the photosynthetic properties did not vary significantly between ontogenetic phases (Figure 1). Therefore the adult-phase-specific plastid H 2 O 2 accumulation may be attributed to shift in scavengers or other factors, excluding changes in photosynthetic rate, because developmental cues like vegetative phase change or physiological aging accelerates ROS production [58].…”

Section: The Accumulation Of Chloroplastic H 2 O 2 In the Adult Phase Is Due To Decreased Scavenging Capacity Rather Than Higher Photosynmentioning

confidence: 99%

MicroRNA156 (miR156) Negatively Impacts Mg-Protoporphyrin IX (Mg-Proto IX) Biosynthesis and Its Plastid-Nucleus Retrograde Signaling in Apple

Zheng

Chen

Jia

et al. 2020

Plants

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Plastid-nucleus retrograde signaling (PNRS) play essential roles in regulating nuclear gene expression during plant growth and development. Excessive reactive oxygen species can trigger PNRS. We previously reported that in apple (Malus domestica Borkh.) seedlings, the expression of microRNA156 (miR156) was significantly low in the adult phase, which was accompanied by high levels of hydrogen peroxide (H2O2) accumulation in chloroplasts. However, it was unclear whether adult-phase-specific chloroplast H2O2 may induce PNRS and affect miR156 expression, or miR156 triggers adult phase PNRS during the ontogenesis. In this paper, we examined the relationship between miR156 levels and six PNRS components in juvenile and adult phase leaves from ‘Zisai Pearl’×‘Red Fuji’ hybrids. We found that PNRS generated by singlet oxygen (1O2), the photosynthetic redox state, methylerythritol cyclodiphosphate (MEcPP), SAL1-3-phosphoadenosine 5-phosphate (PAP) and WHIRLY1 were not involved. The accumulation of Mg-protoporphyrin IX (Mg-Proto IX), the expression of the synthetic genes MdGUN5 and MdGUN6, and Mg-Proto IX PNRS related nuclear genes increased with ontogenesis. These changes were negatively correlated with miR156 expression. Manipulating Mg-Proto IX synthesis with 5-aminolevulinic acid (ALA) or gabaculine did not affect miR156 expression in vitro shoots. In contrast, modulating miR156 expression via MdGGT1 or MdMIR156a6 transgenesis led to changes in Mg-Proto IX contents and the corresponding gene expressions. It was concluded that the Mg-Proto IX PNRS was regulated downstream of miR156 regardless of adult-phase-specific plastid H2O2 accumulation. The findings may facilitate the understanding of the mechanism of ontogenesis in higher plants.

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“…Leaf senescence is a highly regulated developmental process and triggered by diverse environmental factors (Woo et al, 2019 ). As a sophisticated biological event, leaf senescence comprises multidimensional alterations in cell structure, metabolism, and expression of genes (Mayta et al, 2019 ). Organelles and macromolecular substances gradually degraded in an ordered manner.…”

Section: Introductionmentioning

confidence: 99%

TaWRKY13-A Serves as a Mediator of Jasmonic Acid-Related Leaf Senescence by Modulating Jasmonic Acid Biosynthesis

et al. 2021

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Leaf senescence is crucial for crop yield and quality. Transcriptional regulation is a key step for integrating various senescence-related signals into the nucleus. However, few regulators of senescence implicating transcriptional events have been functionally characterized in wheat. Based on our RNA-seq data, we identified a WRKY transcription factor, TaWRKY13-A, that predominately expresses at senescent stages. By using the virus-induced gene silencing (VIGS) method, we manifested impaired transcription of TaWRKY13-A leading to a delayed leaf senescence phenotype in wheat. Moreover, the overexpression (OE) of TaWRKY13-A accelerated the onset of leaf senescence under both natural growth condition and darkness in Brachypodium distachyon and Arabidopsis thaliana. Furthermore, by physiological and molecular investigations, we verified that TaWRKY13-A participates in the regulation of leaf senescence via jasmonic acid (JA) pathway. The expression of JA biosynthetic genes, including AtLOX6, was altered in TaWRKY13-A-overexpressing Arabidopsis. We also demonstrated that TaWRKY13-A can interact with the promoter of AtLOX6 and TaLOX6 by using the electrophoretic mobility shift assay (EMSA) and luciferase reporter system. Consistently, we detected a higher JA level in TaWRKY13-A-overexpressing lines than that in Col-0. Moreover, our data suggested that TaWRKY13-A is partially functional conserved with AtWRKY53 in age-dependent leaf senescence. Collectively, this study manifests TaWRKY13-A as a positive regulator of JA-related leaf senescence, which could be a new clue for molecular breeding in wheat.

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Leaf Senescence: The Chloroplast Connection Comes of Age

Cited by 45 publications

References 145 publications

The rice cytosolic pentatricopeptide repeat protein OsPPR2-1 regulates OsGLK1 to control tapetal plastid development and programmed cell death

The rice cytosolic pentatricopeptide repeat protein OsPPR2-1 regulates OsGLK1 to control tapetal plastid development and programmed cell death

MicroRNA156 (miR156) Negatively Impacts Mg-Protoporphyrin IX (Mg-Proto IX) Biosynthesis and Its Plastid-Nucleus Retrograde Signaling in Apple

TaWRKY13-A Serves as a Mediator of Jasmonic Acid-Related Leaf Senescence by Modulating Jasmonic Acid Biosynthesis

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