Leaf Rust Resistance and Molecular Identification of Lr 34 Gene in Egyptian Wheat

Fahmi, A. I.; Am, El-Shehawi; El-Orabey, Walid

doi:10.4172/1948-5948.1000236

Cited by 6 publications

(2 citation statements)

References 18 publications

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“…Wheat ( Triticum aestivum L.), for instance, is a widely grown crop that feeds around 35% of people worldwide (Deng et al ., 2019 ; Grote et al ., 2021 ). It has been intensively bred to accumulate desirable traits for making elite cultivars with high productivity (Baenziger, 2016 ; Bedő and Láng, 2015 ) and resistance to various disease pathogens, including stem rust (Ai et al ., 2015 ; Aman et al ., 2020c ; Ciuca et al ., 2015 ; Dakouri et al ., 2013 ). Two natural alleles, Lr34 (encoding an ABC transporter) and Lr67 (encoding a hexose‐proton symporter), are widely used in breeding programs to provide broad‐spectrum resistance against rust (Krattinger et al ., 2009 ; Milne et al ., 2018 ; Moore et al ., 2015 ; Spielmeyer et al ., 2013a , b ).…”

Section: Introductionmentioning

confidence: 99%

A CRISPR‐based lateral flow assay for plant genotyping and pathogen diagnostics

Sánchez

Ali

Islam

et al. 2022

Plant Biotechnology Journal

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Efficient pathogen diagnostics and genotyping methods enable effective disease management and breeding, improve crop productivity and ensure food security. However, current germplas m selection and pathogen detection techniques are laborious, time-consuming, expensive, and not easy to mass-scale application in the field. Here, we optimized a field-deployable lateral flow assay, Bio-SCAN, as a highly sensitive tool to precisely identify elite germplasm and detect mutations, transgenes, and phytopathogens in less than 1 hour, starting from sample isolation to result output using lateral flow strips. As a proof of concept, we genotyped various wheat germplasms for the Lr34 and Lr67 alleles conferring broad-spectrum resistance to stripe rust, confirmed the presence of synthetically produced herbicide-resistant alleles in the rice genome, and screened for the presence of transgenic elements in the genome of transgenic tobacco and rice plants with 100% specificity. We also successfully applied this new assay to the detection of phytopathogens, including viruses and bacterial pathogens in Nicotiana benthamiana, and two destructive fungal pathogens (Puccinia striiformis f. sp. tritici and Magnaporthe oryzae Triticum) in wheat. Our results illustrate the power of Bio-SCAN in crop breeding, genetic engineering, and pathogen diagnostics to enhance food security. The high sensitivity, simplicity, versatility, and infield deployability make the Bio-SCAN as an attractive molecular diagnostic tool for diverse applications in agriculture.

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Section: Introductionmentioning

confidence: 99%

A CRISPR‐based lateral flow assay for plant genotyping and pathogen diagnostics

Sánchez

Ali

Islam

et al. 2022

Plant Biotechnology Journal

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“…Twenty-six wheat genotypes were evaluated for the level of plant resistance to leaf rust in two different locations (Shybin El-Kom and Ita-El-Barrow) during two consecutive growing seasons 2011-2013 (Fahmi et al, 2015). Wheat varieties Sids 12, Sids 13, Misr 1 and Misr 2, Shandweel 1, Beni Sweif 4 and Beni Sweif 5 have specific resistance.…”

Section: Introductionmentioning

confidence: 99%

Characteristics of immunity to leaf diseases of winter wheat samples under the conditions of the north-east forest steppe of Ukraine a

Osmachko¹,

Власенко²,

Бакуменко³

et al. 2020

Regul. Mech. Biosyst.

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To realize the genetic potential of the productivity of bread winter wheat varieties, it is necessary to maintain a certain level of plant resistance to disease. Resistance donors may lose this property as a result of changes in the virulence of the pathogen and defeat of the genetic systems of plant resistance. This makes it necessary to search for new resistance sources and donors to leaf diseases. Our researches were conducted using field, laboratory and mathematical-statistical methods. Phenological observations, accounting, evaluation and harvesting were conducted according to currently accepted methods. 86 bread winter wheat samples from the 4th WWSRRN CIMMYT were studied for resistance to leaf diseases in our research during 2014–2016. The manifestation of variability depended significantly on the genotype for three diseases. The highest genotype influence was obseved in resistance to septoria disease, where it was 81%. On average the highest indicator of resistance (7.7) to powdery mildew during the three years of research was observed in the mid-late ripening samples. The mid-early ripening group was considered to be the most adapted to the powdery mildew pathogen in the Northeastern Forest-Steppe. The highest average indicator of resistance (7.5) to brown rust for the three years of research was found in the early ripening group. The samples of the mid-ripening group were most adapted to the brown rust pathogen. The highest average resistance to septoria disease was also found in the early ripening group. The best adaptation to septoria disease was observed in mid-late ripening samples. 36% of the samples were resistant to three diseases. As a result of the research, a number of CIMMYT samples were isolated from the 4th WWSRRN, which exceeded the standard in resistance to powdery mildew, brown rust and septoria disease. They were characterized by better performance. Valuable forms for breeding work that can be resistance donors to leaf diseases were identified among them.

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Phenotypic and molecular characterization of pleiotropic resistance to rusts and powdery mildew in spring wheat

Draz,

Esmail,

Komeil

2024

Euphytica

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An attempt was undertaken to comprehend how phenotypic markers, leaf tip necrosis (LTN), and pseudo black chaff (PBC), are associated with pleiotropic resistance genes in wheat (Triticum spp.). Pleiotropic resistance to stripe rust (Puccinia striiformis f. sp. tritici), leaf rust (P. triticina), stem rust (P. graminis f. sp. tritici) and powdery mildew (Blumeria graminis f. sp. tritici) was evaluated in 57 Egyptian spring wheat cultivars at seedling stage in greenhouse and at adult plant stage in field during 2021/22 and 2022/23. At seedling stage, pleiotropic resistance rated moderately resistance (MR) was observed in five cultivars, Misr-3 (all three rusts), Misr-4 (stripe rust, leaf rust, powdery mildew), Giza-168 (leaf rust, stem rust, powdery mildew), Sakha-94 and Sids-13 (leaf rust, stem rust). At adult plant stage, Misr-4 outperformed all other evaluated cultivars, exhibiting a high level of pleiotropic adult plant resistance (PAPR) against all diseases investigated. High levels of PAPR were also noticed against the three rusts in nine cultivars, Misr-3, Sakha-93, Sakha-94, Sakha-95, Giza-156, Giza-168, Giza-171, Gemmeiza-10 and Sids-13, and moderate levels against powdery mildew in Misr-3, Sakha-95, Giza-156. Four PAPR genes were characterized in cultivars based on phenotypic and molecular markers. Phenotypic markers (LTN and PBC) were observed in 23 cultivars. Molecular marker csLV34 indicated the presence of Lr34/Yr18/Sr57/Pm38/Ltn1 in six cultivars, whilst marker Xwmc44 indicated that Lr46/Yr29/Sr58/Pm39/Ltn2 was present in five cultivars. Marker Xcfd71 indicated the presence of Lr67/Yr46/Sr55/Pm46/Ltn3 in 19 cultivars. Gene Sr2/Yr30/Lr27/Pbc was identified with marker csSr2 in three cultivars. A complete association between LTN and molecular markers was recorded, while a strong association (r = 0.73) was recorded between PBC and molecular markers. Findings demonstrated the reliability of phenotypic markers in predicting pleiotropic resistance in wheat, which would facilitate marker-assisted selection in breeding programs.

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Leaf Rust Resistance and Molecular Identification of Lr 34 Gene in Egyptian Wheat

Cited by 6 publications

References 18 publications

A CRISPR‐based lateral flow assay for plant genotyping and pathogen diagnostics

A CRISPR‐based lateral flow assay for plant genotyping and pathogen diagnostics

Characteristics of immunity to leaf diseases of winter wheat samples under the conditions of the north-east forest steppe of Ukraine a

Phenotypic and molecular characterization of pleiotropic resistance to rusts and powdery mildew in spring wheat

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