Leaf protein for food use: potential of Rubisco

Douillard, Roger; Mathan, O. de

doi:10.1007/978-1-4615-2652-0_10

Cited by 17 publications

(27 citation statements)

References 151 publications

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“…RuBisCO (ribuloz 1,5-bifosfat karboksilaz/oksijenaz) çözünebilir yaprak proteininin %30-70'ini oluşturmakta (Douillard and Mathan 1994) ve fotosentezde önemli rol oynamaktadır. RuBisCO, karbon metabolizmasının ilk basamağı olan CO 2 fiksasyonunu kataliz etmektedir.…”

Section: Karbondioksit öZümlemesi Ve Fotosentez Ile İlişkili Proteinlerunclassified

Heavy Metal Toxicity in Plants: Proteomics Approach

Terzi¹,

Yıldız²

2013

AKU-J. Sci. Eng.

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ÖzetAğır metal kirliliği, insan sağlığını olumsuz etkileyen ve tarımsal verimde kayıplara neden olan önemli çevresel tehlikelerden biridir. Bitki hücresinin en önemli fonksiyonu, kendi savunması için ağır metal stresine karşı cevap vermektir. Ağır metal stresine cevap olarak fonksiyonel genler veya proteinlerin teşhisi stres cevaplarının moleküler mekanizmalarının anlaşılmasında önemli bir basamaktır. Protein ekspresyonu sadece transkripsiyonel seviyede değil, aynı zamanda translasyonel ve post-translasyonel seviyelerde de düzenlenmektedir. Bu nedenle, farklı stres koşulları altında bitki proteomundaki değişimleri araştırmak önemlidir. Genom tarafından eksprese olan proteinlerin geniş ölçekli analizi olarak tanımlanan proteomik, sadece proteomun tamamını tanımlamak için değil, aynı zamanda ağır metal stresi gibi farklı fizyolojik koşullar altındaki proteomların karşılaştırılması için güçlü bir moleküler araçtır. Bu derlemede, proteomik teknolojileri ve bitkilerde farklı metabolik olaylarda rolü olan ağır metal teşvikli proteinler tartışılmıştır. Heavy Metal Toxicity in Plants: Proteomics Approach Key wordsPlants; Heavy Metal; Toxicity; Proteomics; Mass Spectrometry AbstractHeavy metal contamination is one of the major environmental hazards, leading to losses in agricultural yields and harmfully affecting human health. The most crucial function of plant cell is to respond against heavy metal stress for self-defence. The identification of the functional genes or proteins that are involved in responses to heavy-metal stress is a fundamental step in understanding the molecular mechanisms of stress responses. Protein expression is regulated not only at the transcriptional level, but also at the translational and post-translational levels. Therefore, it is crucial to investigate the changes in plant proteome under different stress conditions. Proteomics, defined as the large-scale analysis of proteins expressed by the genome, is not only a powerful molecular tool for describing complete proteome, but also for comparative proteomes under different physiological conditions, such as heavy metal stress. In this review, proteomics technologies, and heavy metal induced proteins which play a role in different metabolic events in plants are discussed.

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Section: Karbondioksit öZümlemesi Ve Fotosentez Ile İlişkili Proteinlerunclassified

Heavy Metal Toxicity in Plants: Proteomics Approach

Terzi¹,

Yıldız²

2013

AKU-J. Sci. Eng.

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“…Entretanto, muitas pesquisas foram conduzidas na Europa e nos Estados-Unidos para a preparação de concentrados protéicos a partir de outras variedades de planta. Já existem grandes empresas na França, Itália e Alemanha, que produzem em escala industrial concentrado protéico de alfafa (16,19,20,27).…”

Section: Aspectos Tecnológicosunclassified

As Proteínas De Folha De Mandioca: Aspectos Fisiológicos, Nutricionais E Importância Tecnológica

Guérroue

Douillard²,

Cereda³

et al. 1996

B. do CEPPA

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Alguns aspectos ligados à tecnologia de proteínas de folhas, com ênfase para as folhas de mandioca (Manihot esculenta Crantz), são revisados. A composição em aminoácidos, as propriedades bioquímicas, a importância nutricional e os princípios dos métodos de extração são apresentados. As folhas de mandioca apresentam elevado teor de proteínas (20-30% base seca), de valor nutricional adequado às recomendações da FAO, além de altos teores de vitaminas A e C e de minerais. Algumas aplicações potenciais em nutrição humana ou animal ou como substrato para a indústria de biotecnologia são discutidas.

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“…RuBisCO is composed of eight large and eight small subunits with molecular weight approx. 55 and 13 kDa, respectively [8,9]. RuBisCO was reported as one of the most abundant proteins in the biosphere [10,11], and thus it is of particular interest for green biomass biorefinery.…”

Section: Introductionmentioning

confidence: 99%

Enzyme Enhanced Protein Recovery from Green Biomass Pulp

Доценко

Lange

2016

Waste Biomass Valor

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Globally, animal feed protein is a key factor for production of meat for human consumption. Protein for animal feed is in many parts of the world not available in sufficient amounts; demand is met only through import of feed protein. Such protein deficit can be minimized through optimized use of local protein resources based on upgrade from e.g. green plant biomass. In present work we consider different strategies for protein recovery from white clover and ryegrass screw press pulps, using aqueous extraction, as well as carbohydrases and proteases enhanced extraction. Protein recovery in these studies was determined as a yield of solubilized protein with regard to the total protein in a screw press pulp. Aqueous extraction at pH 8.0 resulted in approx. 40 % protein recovery, while proteases application (Savinase 16.0L, Novozymes) enabled twice higher protein yield. Application of plant cell wall degrading enzymes (Cellic CTec2 and Cellic HTec2, Novozymes) did not provide detectable protein recovery, while consecutive proteases treatment resulted in approx. 95 % protein yield. RuBisCO peptides were demonstrated by amino acid analysis to be the major component of white clover and ryegrass pulp proteolyzates, generated by Savinase 16.0L protease.

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Leaf protein for food use: potential of Rubisco

Cited by 17 publications

References 151 publications

Heavy Metal Toxicity in Plants: Proteomics Approach

Heavy Metal Toxicity in Plants: Proteomics Approach

As Proteínas De Folha De Mandioca: Aspectos Fisiológicos, Nutricionais E Importância Tecnológica

Enzyme Enhanced Protein Recovery from Green Biomass Pulp

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