Leaf Disc Regeneration of Passion Fruit

Amugune, Nelson Onzere; Gopalan, H. N. B.; Bytebier, Benny

doi:10.4314/acsj.v1i2.69896

Cited by 11 publications

(15 citation statements)

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“…Marcin and Barbara (2013) reported micropropagation for Passiflora spp of medicinal plants. However, given the species and/or variety-specific response to in vitro culture media and conditions (Drew, 1991, Amugune, 1993Arinaitwe, 2008;Marcin and Barbara, 2013), it is appropriate to optimize a system for production of commercial varieties. Therefore, the aim of this study was to determine optimum in vitro growth conditions for micropropagation of the three commercial passionfruit varieties in Uganda.…”

Section: Introductionmentioning

confidence: 99%

<i>In vitro</i> propagation of three commercial passionfruit varieties in Uganda

Mukasa¹,

Ssamula²,

Asami³

et al. 2016

Afr. Crop Sci. J.

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Section: Introductionmentioning

confidence: 99%

<i>In vitro</i> propagation of three commercial passionfruit varieties in Uganda

Mukasa¹,

Ssamula²,

Asami³

et al. 2016

Afr. Crop Sci. J.

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“…There are a number of plant regeneration reports available on Passiflora using various explants and plant growth regulators combinations [14], [15]. Reference [16] reported plant regeneration through leaf discs of passion fruit using kinetin, BAP, and NAA. They also discussed that callus and roots could be induced in leaf discs of Passiflora edulis Sims f. flavicarpa Deg.…”

Section: Discussionmentioning

confidence: 99%

Scanning Electron Microscopy Studies and In Vitro Regeneration of Passiflora edulis Sims var. edulis for Conservation

Veeramohan¹,

Haron²,

Taha³

2013

IJESD

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“…In Passiflora, in vitro propagation studies began in 1966 (Nakayama 1966). Additional approaches were later published for distinct Passiflora species, particularly using direct (Dornelas and Vieira 1994;Anand et al 2012) or indirect organogenesis (Amugune et al 1993;Vieira et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Embryogenic potential of immature zygotic embryos of Passiflora: a new advance for in vitro propagation without plant growth regulators

Ferreira

Sattler

Carvalho

et al. 2015

Plant Cell Tiss Organ Cult

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In vitro strategies for Passiflora have been developed owing to its economic and ecological importance. However, plantlet regeneration through somatic embryogenesis has presented some problems, such as the reproducibility of the protocol and formation of abnormal embryos and plantlets. Thus, this study aimed to establish a protocol exploring the embryogenic potential of immature zygotic embryos (IZE) of the wild species Passiflora miniata Vanderpl. and Passiflora speciosa Gardn. Friable calli, which formed on the abaxial surface of the cotyledons, yielded globular, heart-shaped, torpedo and cotyledonary somatic embryos, characterising the embryogenic response as asynchronous. A high percentage of normal regenerants (90 %) was obtained from IZE in media lacking 2,4-dichlorophenoxyacetic acid (2,4-D) in comparison to the value of normal plantlets (60 %) regenerated from mature zygotic embryos inoculated in media with 2,4-D. This result demonstrates that IZE of P. miniata and P. speciosa possess sufficient levels of endogenous phytohormones to trigger a high rate of indirect somatic embryogenesis. All regenerated plantlets had the same genome size and chromosome number as the explant donor plants. Therefore, the indirect embryogenic pathway, employing IZE inoculated into media free of growth regulators, did not cause changes in the karyotype and morphology. Based on these results, IZE should be considered as explant for the establishment of somatic embryogenesis in other species. Besides, a new, reliable and relatively rapid protocol to recover plantlets of P. miniata and P. speciosa yielded several plants, which were acclimatised and used for ornamental purposes and breeding programs, and for reintroduction into biological reserves.

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Leaf Disc Regeneration of Passion Fruit

Cited by 11 publications

References 0 publications

<i>In vitro</i> propagation of three commercial passionfruit varieties in Uganda

<i>In vitro</i> propagation of three commercial passionfruit varieties in Uganda

Scanning Electron Microscopy Studies and In Vitro Regeneration of Passiflora edulis Sims var. edulis for Conservation

Embryogenic potential of immature zygotic embryos of Passiflora: a new advance for in vitro propagation without plant growth regulators

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