&lt;i&gt;In vitro&lt;/i&gt; propagation of three commercial passionfruit varieties in Uganda

Mukasa, S.B.; Ssamula, Alexander; Asami, Pauline; Holton, Timothy A

doi:10.4314/acsj.v24i4.6

Cited by 2 publications

(2 citation statements)

References 8 publications

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“…is finding supports the assumption that shoot organogenesis in passion fruit is genotype-specific. e genotypic effect on the shoot organogenesis of passion fruit varieties tested in this study was also observed by Amugune et al [30] and Mukasa et al [37].…”

Section: Discussionsupporting

confidence: 87%

Efficient Shoot Organogenesis Using Leaf Disc and Nodal Explants of Passion Fruit (Passiflora edulisSims) and Genetic Fidelity Assessment Using Sequence-Related Amplified Polymorphism (SRAP) Markers

Asande

Ombori

Nyaboga

et al. 2020

International Journal of Agronomy

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Passion fruit (Passiflora edulis (Sims)) is currently ranked third among fruit exports from Kenya and has great potential since the demand for both fresh fruit and processed juice is on a continuous increase. Passion fruit production in Kenya is constrained by a lack of healthy, clean planting material, poor seed viability, and low germination rates. To address this, the present study reports an in vitro plant regeneration protocol for passion fruit using leaf disc and nodal explants and genetic fidelity analysis of the regenerated plants. The highest number of shoot regeneration was obtained on Murashige and Skoog (MS) medium supplemented with 2 mg·L−1 6-Benzyl amino purine (BAP) (shoot induction medium). The multiplication of shoots was optimum in MS medium supplemented with 3 mg·L−1 BAP. To eliminate the requirement of an additional step of in vitro rooting, exogenous application of putrescine induced the formation and development of roots on nodal explants. Genetic fidelity analysis of the in vitro regenerated and macropropagated plants with that of the mother plant was carried out by sequence-related amplified polymorphism (SRAP) markers, and monomorphic banding profile for 80% of the regenerants confirmed the genetic uniformity of the in vitro regenerated and macropropagated plants. The in vitro regeneration system developed can be utilized for mass clonal propagation for the economic commercial exploitation of this important tropical fruit.

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Section: Discussionsupporting

confidence: 87%

Efficient Shoot Organogenesis Using Leaf Disc and Nodal Explants of Passion Fruit (Passiflora edulisSims) and Genetic Fidelity Assessment Using Sequence-Related Amplified Polymorphism (SRAP) Markers

Asande

Ombori

Nyaboga

et al. 2020

International Journal of Agronomy

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“…These results show that callus induction and somatic embryogenesis in passion fruit is genotype-dependent. The genotypic effect on the response of passion fruit varieties to in vitro regeneration has also been reported by Amugune et al [25] and Mukasa et al [26].…”

Section: Responses Of Leaf Explants Of Kpf4 and Purple Passion Fruit ...supporting

confidence: 64%

Somatic Embryogenesis and Plant Regeneration of Farmer-Preferred Passion Fruit Varieties Grown in Kenya

Asande,

Ombori,

Oduor

et al. 2023

IJPB

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In vitro regeneration of passion fruit has great prospects for mass production of disease-free planting materials. The objective of this study was to develop an in vitro regeneration system through somatic embryogenesis for farmer-preferred genotypes grown in Kenya. Callus induction and somatic embryogenesis were carried out using leaf and immature seed explants. The explants were cultured on Murashige and Skoog medium augmented with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) alone and 2,4-D combined with 1.0 mg L−1 thidiazuron (TDZ). Data were recorded and subjected to analysis of variance. The highest number of somatic embryos was obtained from KPF4 genotype using MS medium supplemented with 8 mg L−1 2,4-D and 1 mg L−1 TDZ. The embryos were converted to plants on germination medium comprising of MS augmented with 0.5 mg L−1 6-benzyl amino purine (BAP). The plantlets were hardened for 4 weeks in plastic pots. The survival rate during hardening of in vitro regenerated plants was 77.8%. The present study reports a regeneration system through somatic embryogenesis for KPF4 passion fruit grown in Kenya. The in vitro regeneration system can be utilized for mass propagation and genetic improvement of KPF4 variety grown in Kenya.

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<i>In vitro</i> propagation of three commercial passionfruit varieties in Uganda

Cited by 2 publications

References 8 publications

Efficient Shoot Organogenesis Using Leaf Disc and Nodal Explants of Passion Fruit (Passiflora edulisSims) and Genetic Fidelity Assessment Using Sequence-Related Amplified Polymorphism (SRAP) Markers

Efficient Shoot Organogenesis Using Leaf Disc and Nodal Explants of Passion Fruit (Passiflora edulisSims) and Genetic Fidelity Assessment Using Sequence-Related Amplified Polymorphism (SRAP) Markers

Somatic Embryogenesis and Plant Regeneration of Farmer-Preferred Passion Fruit Varieties Grown in Kenya

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