Leaf cDNA-AFLP analysis reveals novel mechanisms for boron-induced alleviation of aluminum-toxicity in Citrus grandis seedlings

Wang, Liuqing; Yang, Lin-Tong; Guo, Peng; Zhou, Xin-Xing; Ye, Xin; Chen, En-Jun; Chen, Li‐Song

doi:10.1016/j.ecoenv.2015.06.009

Cited by 20 publications

(9 citation statements)

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“…We identified five upregulated transcription factors (TFs) (i.e., probable WRKY TF 50, basic-leucine zipper (bZIP) TF family protein, nuclear factor Y (NFY), subunit C11, C2H2-like zinc finger protein and HAP3-like protein), one downregulated TF (Myb domain protein 15), two upregulated (i.e., methyl-CPG-binding domain 11 and GLNB1 homolog) and two downregulated (i.e., zinc knuckle (CCHC-type) family protein and mitochondrial transcription termination factor family protein) proteins related to transcription regulation in +Al C. sinensis leaves ( Table S1 ). Regulatory genes have crucial roles in plant tolerance to abiotic stresses, including Al toxicity [ 6 , 7 , 43 , 44 ]. Three zinc finger TFs, AtSTOP1 (sensitive to proton rhizotoxicity), OsART1 (Al resistance TF) and TaSTOP1 , have been cloned from Arabidopsis , rice and bread wheat, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…Signal transduction is altered by Al toxicity [ 6 , 7 , 13 ]. As expected, we isolated five upregulated and one downregulated protein related to protein phosphorylation/dephosphorylation, four upregulated Ca signal-related proteins and another five proteins (i.e., four upregulated and one downregulated) involved in signal transduction from +Al C. sinensis leaves ( Table S1 ).…”

Section: Discussionmentioning

confidence: 99%

“…Higher plants have evolved two main mechanisms of Al detoxification (i.e., external and internal detoxification mechanisms) that enable them to tolerate high levels of Al. However, the molecular mechanisms for Al tolerance in higher plants are not fully understood [ 4 , 6 , 7 , 8 , 9 ]. The alteration of proteins is an important process to cope with elevated Al in higher plants [ 7 , 10 , 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Aluminum Toxicity-Induced Alterations of Leaf Proteome in Two Citrus Species Differing in Aluminum Tolerance

Yang

et al. 2016

IJMS

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Seedlings of aluminum-tolerant ‘Xuegan’ (Citrus sinensis) and Al-intolerant ‘sour pummelo’ (Citrus grandis) were fertigated for 18 weeks with nutrient solution containing 0 and 1.2 mM AlCl3·6H2O. Al toxicity-induced inhibition of photosynthesis and the decrease of total soluble protein only occurred in C. grandis leaves, demonstrating that C. sinensis had higher Al tolerance than C. grandis. Using isobaric tags for relative and absolute quantification (iTRAQ), we obtained more Al toxicity-responsive proteins from C. sinensis than from C. grandis leaves, which might be responsible for the higher Al tolerance of C. sinensis. The following aspects might contribute to the Al tolerance of C. sinensis: (a) better maintenance of photosynthesis and energy balance via inducing photosynthesis and energy-related proteins; (b) less increased requirement for the detoxification of reactive oxygen species and other toxic compounds, such as aldehydes, and great improvement of the total ability of detoxification; and (c) upregulation of low-phosphorus-responsive proteins. Al toxicity-responsive proteins related to RNA regulation, protein metabolism, cellular transport and signal transduction might also play key roles in the higher Al tolerance of C. sinensis. We present the global picture of Al toxicity-induced alterations of protein profiles in citrus leaves, and identify some new Al toxicity-responsive proteins related to various biological processes. Our results provide some novel clues about plant Al tolerance.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Aluminum Toxicity-Induced Alterations of Leaf Proteome in Two Citrus Species Differing in Aluminum Tolerance

Yang

et al. 2016

IJMS

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“…Boron can act synergically with Ca and prevent binding of Al to the cell wall ( Hossain et al, 2005 ; Yu et al, 2009b ; Horst et al, 2010 ). Recent evidence in pomelo ( Citrus grandis ) suggests that B prevents Al-toxicity by regulating different genes associated with modification of the cell wall, cellular transport, metabolism, signal transduction and antioxidant activity ( Wang et al, 2015c ; Zhou et al, 2015 ). Similarly, the addition of P prevents the effect of Al-toxicity on root growth and photosynthetic machinery of C. grandis ( Jianq et al, 2009 ).…”

Section: Mechanisms Of Tolerance To Aluminummentioning

confidence: 99%

Aluminum, a Friend or Foe of Higher Plants in Acid Soils

et al. 2017

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Aluminum (Al) is the most abundant metal in the earth’s crust, but its availability depends on soil pH. Despite this abundance, Al is not considered an essential element and so far no experimental evidence has been put forward for a biological role. In plants and other organisms, Al can have a beneficial or toxic effect, depending on factors such as, metal concentration, the chemical form of Al, growth conditions and plant species. Here we review recent advances in the study of Al in plants at physiological, biochemical and molecular levels, focusing mainly on the beneficial effect of Al in plants (stimulation of root growth, increased nutrient uptake, the increase in enzyme activity, and others). In addition, we discuss the possible mechanisms involved in improving the growth of plants cultivated in soils with acid pH, as well as mechanisms of tolerance to the toxic effect of Al.

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“…Gene expression networks unraveled by transcriptomics give us the chance to understand the mechanisms of Al-toxicity and Al-tolerance in higher plants (Chandran et al, 2008; Kumari et al, 2008; Maron et al, 2008; Fan et al, 2014; Wang et al, 2015; Zhou et al, 2015). Recently, a high-throughput sequencing method [RNA sequencing (RNA-Seq)] is developed to analyze the transcriptome prior to the sequencing of the genome.…”

Section: Introductionmentioning

confidence: 99%

Root Adaptive Responses to Aluminum-Treatment Revealed by RNA-Seq in Two Citrus Species With Different Aluminum-Tolerance

Guo

Yang

et al. 2017

Front. Plant Sci.

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Seedlings of aluminum (Al)-tolerant Citrus sinensis and Al-intolerant Citrus grandis were fertigated daily with nutrient solution containing 0 and 1.0 mM AlCl3●6H2O for 18 weeks. The Al-induced decreases of biomass and root total soluble proteins only occurred in C. grandis, demonstrating that C. sinensis had higher Al-tolerance than C. grandis. Under Al-treatment, C. sinensis roots secreted more citrate and malate than C. grandis ones; less Al was accumulated in C. sinenis than in C. grandis leaves. The Al-induced reduction of phosphorus was lesser in C. sinensis roots and leaves than in C. grandis ones, whereas the Al-induced increase of sulfur was greater in C. sinensis roots and leaves. Using RNA-seq, we isolated 1905 and 2670 differentially expressed genes (DEGs) from Al-treated C. sinensis than C. grandis roots, respectively. Among these DEGs, only 649 DEGs were shared by the two species. Further analysis suggested that the following several aspects conferred C. sinensis higher Al-tolerance: (a) Al-treated C. sinensis seedlings had a higher external Al detoxification capacity via enhanced Al-induced secretion of organic acid anions, a higher antioxidant capacity and a more efficient chelation system in roots; (b) Al-treated C. sinensis seedlings displayed a higher level of sulfur in roots and leaves possibly due to increased uptake and decreased export of sulfur and a higher capacity to maintain the cellular phosphorus homeostasis by enhancing phosphorus acquisition and utilization; (c) Cell wall and cytoskeleton metabolism, energy and carbohydrate metabolism and signal transduction displayed higher adaptative responses to Al in C. sinensis than in C. grandis roots; (d) More upregulated than downregulated genes related to fatty acid and amino acid metabolisms were isolated from Al-treated C. sinensis roots, but the reverse was the case for Al-treated C. grandis roots. These results provide a platform for further investigating the roles of genes possibly responsible for citrus Al-tolerance.

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Leaf cDNA-AFLP analysis reveals novel mechanisms for boron-induced alleviation of aluminum-toxicity in Citrus grandis seedlings

Cited by 20 publications

References 93 publications

Aluminum Toxicity-Induced Alterations of Leaf Proteome in Two Citrus Species Differing in Aluminum Tolerance

Aluminum Toxicity-Induced Alterations of Leaf Proteome in Two Citrus Species Differing in Aluminum Tolerance

Aluminum, a Friend or Foe of Higher Plants in Acid Soils

Root Adaptive Responses to Aluminum-Treatment Revealed by RNA-Seq in Two Citrus Species With Different Aluminum-Tolerance

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