Leaf age‐related changes in respiratory pathways are dependent on complex I activity in <i>Nicotiana sylvestris</i>

Priault, Pierrick; Vidal, Guillaume; Paepe, Rosine De; Ribas‐Carbó, Miquel

doi:10.1111/j.1399-3054.2006.00836.x

Cited by 36 publications

(33 citation statements)

References 62 publications

(99 reference statements)

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“…Both increased amounts of organic acids and higher levels of pyridine nucleotide can be attributed to an increased rate of respiration (Priault et al, 2007). The metabolite profile suggests that the changed pyridine nucleotide levels in old5 lead to an altered respiration rate.…”

Section: Increased Respiration Rate and Antioxidant Accumulation In Old5mentioning

confidence: 97%

TheArabidopsis onset of leaf death5Mutation of Quinolinate Synthase Affects Nicotinamide Adenine Dinucleotide Biosynthesis and Causes Early Ageing

et al. 2008

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Leaf senescence in Arabidopsis thaliana is a strict, genetically controlled nutrient recovery program, which typically progresses in an age-dependent manner. Leaves of the Arabidopsis onset of leaf death5 (old5) mutant exhibit early developmental senescence. Here, we show that OLD5 encodes quinolinate synthase (QS), a key enzyme in the de novo synthesis of NAD. The Arabidopsis QS was previously shown to carry a Cys desulfurase domain that stimulates reconstitution of the oxygen-sensitive Fe-S cluster that is required for QS activity. The old5 lesion in this enzyme does not affect QS activity but it decreases its Cys desulfurase activity and thereby the long-term catalytic competence of the enzyme. The old5 mutation causes increased NAD steady state levels that coincide with increased activity of enzymes in the NAD salvage pathway. NAD plays a key role in cellular redox reactions, including those of the tricarboxylic acid cycle. Broad-range metabolite profiling of the old5 mutant revealed that it contains higher levels of tricarboxylic acid cycle intermediates and nitrogen-containing amino acids. The mutant displays a higher respiration rate concomitant with increased expression of oxidative stress markers. We postulate that the alteration in the oxidative state is integrated into the plant developmental program, causing early ageing of the mutant.

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Section: Increased Respiration Rate and Antioxidant Accumulation In Old5mentioning

confidence: 97%

TheArabidopsis onset of leaf death5Mutation of Quinolinate Synthase Affects Nicotinamide Adenine Dinucleotide Biosynthesis and Causes Early Ageing

et al. 2008

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“…By itself, this should cause decreased ADP/O ratios in CMSII. However, increased respiration in CMSII only involves the cytochrome oxidase (COX) pathway, at the expense of Xux through the alternative oxidase (AOX: Vidal et al 2007;Priault et al 2007). This could maintain or even increase ADP/O ratios in CMSII, and it is worth noting that ATP levels are higher in CMSII (Szal et al 2008).…”

Section: High Co 2 Exerts Modest Evects On the Cmsii Metabolic Signaturementioning

confidence: 99%

Conditional modulation of NAD levels and metabolite profiles in Nicotiana sylvestris by mitochondrial electron transport and carbon/nitrogen supply

Hager

Pellny

Mauve

et al. 2010

Planta

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Environmental controls on leaf NAD status remain poorly understood. Here, we analyzed the effects of two key environmental variables, CO(2) and nitrogen, on leaf metabolite profiles, NAD status and the abundance of key transcripts involved in de novo NAD synthesis in wild-type (WT) Nicotiana sylvestris and the CMSII mutant that lacks respiratory complex I. High CO(2) and increased N supply both significantly enhanced NAD(+) and NADH pools in WT leaves. In nitrogen-sufficient conditions, CMSII leaves were enriched in NAD(+) and NADH compared to the WT, but the differences in NADH were smaller at high CO(2) than in air because high CO(2) increased WT NADH/NAD(+). The CMSII-linked increases in NAD(+) and NADH status were abolished by growth with limited nitrogen, which also depleted the nicotine and nicotinic acid pools in the CMSII leaves. Few statistically significant genotype and N-dependent differences were detected in NAD synthesis transcripts, with effects only on aspartate oxidase and NAD synthetase mRNAs. Non-targeted metabolite profiling as well as quantitative amine analysis showed that NAD(+) and NADH contents correlated tightly with leaf amino acid contents across all samples. The results reveal considerable genotype- and condition-dependent plasticity in leaf NAD(+) and NADH contents that is not linked to modified expression of NAD synthesis genes at the transcript level and show that NAD(+) and NADH contents are tightly integrated with nitrogen metabolism. A regulatory two-way feedback circuit between nitrogen and NAD in the regulation of N assimilation is proposed that potentially links the nutritional status to NAD-dependent signaling pathways.

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“…In summary, there is an induction of alternative respiratory pathways in ndufs8.1 ndufs8.2 similar to most CI mutants investigated so far. However, in contrast to CMSII (Priault et al, 2007;Vidal et al, 2007), the in vivo electron partition to AOX is increased in ndufs8.1 ndufs8.2.…”

Section: Lack Of the Ndufs8 Subunit Results In Holo-ci Misassembly Anmentioning

confidence: 88%

“…Also, despite differences in tricarboxylic acid derivatives and some amino acids upon transfer from SD to LD, there is limited metabolic adaptation to LD in both mutants, in agreement with their impaired growth response. The remodeled metabolomes of CMSII Dutilleul et al, 2003a;Priault et al, 2006aPriault et al, , 2007 and ndufs4 (Meyer et al, 2009) mutants were associated with alterations in carbon and nitrogen assimilation under the LD condition. Here, we found that although net CO 2 assimilation (A) was lower in the ndufs8.1 ndufs8.2 mutant than in the wild type under elevated light and was stimulated by LD in both genotypes (light response curves shown in Supplemental Fig.…”

Section: Development Of Single and Double Mutants For The Ndufs8 Subumentioning

confidence: 99%

“…Differential impact of the mutations also was observed at the physiological and metabolic levels. Decreased photosynthetic activity is not a general effect and was reported in the N. sylvestris CMSII and NMS1 mutants Dutilleul et al, 2003a;Priault et al, 2007) and in the Arabidopsis ca2 cal2 mutant only (Soto et al, 2015). In the N. sylvestris mutants, limited carbon supply resulting from higher photorespiration is likely to contribute to the slowgrowth phenotype (Priault et al, 2006b).…”

mentioning

confidence: 99%

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Photoperiod Affects the Phenotype of Mitochondrial Complex I Mutants

Pétriacq

Bont²,

Genestout³

et al. 2016

Plant Physiol.

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Plant mutants for genes encoding subunits of mitochondrial complex I (CI; NADH:ubiquinone oxidoreductase), the first enzyme of the respiratory chain, display various phenotypes depending on growth conditions. Here, we examined the impact of photoperiod, a major environmental factor controlling plant development, on two Arabidopsis (Arabidopsis thaliana) CI mutants: a new insertion mutant interrupted in both ndufs8.1 and ndufs8.2 genes encoding the NDUFS8 subunit and the previously characterized ndufs4 CI mutant. In the long day (LD) condition, both ndufs8.1 and ndufs8.2 single mutants were indistinguishable from Columbia-0 at phenotypic and biochemical levels, whereas the ndufs8.1 ndufs8.2 double mutant was devoid of detectable holo-CI assembly/activity, showed higher alternative oxidase content/activity, and displayed a growth retardation phenotype similar to that of the ndufs4 mutant. Although growth was more affected in ndufs4 than in ndufs8.1 ndufs8.2 under the short day (SD) condition, both mutants displayed a similar impairment of growth acceleration after transfer to LD compared with the wild type. Untargeted and targeted metabolomics showed that overall metabolism was less responsive to the SD-to-LD transition in mutants than in the wild type. The typical LD acclimation of carbon and nitrogen assimilation as well as redox-related parameters was not observed in ndufs8.1 ndufs8. Similarly, NAD(H) content, which was higher in the SD condition in both mutants than in Columbia-0, did not adjust under LD. We propose that altered redox homeostasis and NAD(H) content/redox state control the phenotype of CI mutants and photoperiod acclimation in Arabidopsis.Complex I (CI; NADH:ubiquinone oxidoreductase; EC 1.6.5.3), the first enzyme of the respiratory chain of most eukaryotes, including plants, couples electron transfer to proton translocation through the inner mitochondrial membrane (Klodmann et al., 2010). CI is an L-shaped multimeric enzyme of around 1 MD in size composed of a matrix-faced peripheral arm carrying the NADH-oxidizing activity (N module), a connecting module (Q module transferring electrons to the quinonebinding site), and a hydrophobic intramembrane arm carrying the proton translocation activity (P module). Eukaryotic CI comprises more than 40 subunits, 434

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Leaf age‐related changes in respiratory pathways are dependent on complex I activity in Nicotiana sylvestris

Cited by 36 publications

References 62 publications

TheArabidopsis onset of leaf death5Mutation of Quinolinate Synthase Affects Nicotinamide Adenine Dinucleotide Biosynthesis and Causes Early Ageing

TheArabidopsis onset of leaf death5Mutation of Quinolinate Synthase Affects Nicotinamide Adenine Dinucleotide Biosynthesis and Causes Early Ageing

Conditional modulation of NAD levels and metabolite profiles in Nicotiana sylvestris by mitochondrial electron transport and carbon/nitrogen supply

Photoperiod Affects the Phenotype of Mitochondrial Complex I Mutants

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