Lead poisoning. Further observations on erythrocyte pyrimidine-nucleotidase deficiency and intracellular accumulation of pyrimidine nucleotides.

Paglia, Donald E.; Valentine, William N.; Fink, Kay

doi:10.1172/jci108896

Cited by 57 publications

(23 citation statements)

References 16 publications

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“…This has been reported in lead workers, with the greatest inhibition and marked accumulations of pyrimidine nucleotides apparent in workers with overt intoxication, including anemia (Paglia et al 1975(Paglia et al , 1977. PbBs in these workers ranged between 45 and 110 μg/dL, and 7 of 9 were anemic.…”

Section: Health Effectsmentioning

confidence: 68%

“…Another potential biomarker for hematologic effects of lead is the observation of basophilic stippling and premature erythrocyte hemolysis (Paglia et al 1975(Paglia et al , 1977. Lead can impair the activity of pyrimidine 5'-nucleotidase, resulting in a corresponding increase in pyrimidine nucleotides in red blood cells, which leads to a deficiency in maturing erythroid elements and thus, decreased red blood cells.…”

Section: Biomarkers Used To Characterize Effects Caused By Leadmentioning

confidence: 99%

“…Lead can impair the activity of pyrimidine 5'-nucleotidase, resulting in a corresponding increase in pyrimidine nucleotides in red blood cells, which leads to a deficiency in maturing erythroid elements and thus, decreased red blood cells. However, this effect is nonspecific; it is encountered with benzene and arsenic poisoning (Smith et al 1938) and in a genetically-induced enzyme-deficiency syndrome (Paglia et al 1975(Paglia et al , 1977. Furthermore, since basophilic stippling is not universally found in chronic lead poisoning, it is relatively insensitive to lesser degrees of lead toxicity (CDC 1985).…”

Section: Biomarkers Used To Characterize Effects Caused By Leadmentioning

confidence: 99%

See 2 more Smart Citations

Characterizing Golden Eagle Risk to Lead and Anticoagulant Rodenticide Exposure: A Review

Herring

Eagles‐Smith

Buck

2017

Journal of Raptor Research

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Section: Health Effectsmentioning

confidence: 68%

Section: Biomarkers Used To Characterize Effects Caused By Leadmentioning

confidence: 99%

Section: Biomarkers Used To Characterize Effects Caused By Leadmentioning

confidence: 99%

See 1 more Smart Citation

Characterizing Golden Eagle Risk to Lead and Anticoagulant Rodenticide Exposure: A Review

Herring

Eagles‐Smith

Buck

2017

Journal of Raptor Research

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“…A lead-induced deficiency of P5N-I also results in the induction of basophilic stippling and premature erythrocyte hemolysis analogous to that encountered in genetically induced enzyme-deficiency syndrome [17,18,22]. Treatment of erythrocytes with lead also inhibits canine P5N-I [8], resulting in retardation of the morphological reticulocyte maturation [7].…”

Section: Discussionmentioning

confidence: 96%

Inhibitory Effect of Pyrimidine and Purine Nucleotides on the Multiplication of <i>Babesia gibsoni</i>: Possible Cause of Low Parasitemia and Simultaneous Reticulocytosis in Canine Babesiosis

Hossain

Yamato

Yamasaki

et al. 2004

The Journal of Veterinary Medical Science

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ABSTRACT. The present study was conducted to determine the cause of low parasitemia and simultaneous reticulocytosis in canine babesiosis. The parasitemia was significantly decreased in in vitro cultures of Babesia gibsoni by the pretreatment of host canine erythrocytes with lead acetate, which is a specific inhibitor of pyrimidine 5'-nucleotidase subclass I (P5N-I). The serum from dogs chronically infected with B. gibsoni did not decrease the activities of hexokinase, glucose-6-phosphate dehydrogenase or 6-phosphogluconate dehydrogenase in canine reticulocytes, although it was previously reported that this serum had inhibitory effects on both the maturation of reticulocytes and the canine P5N-I and purine-specific 5'-nucleotidase activities. Furthermore, the in vitro multiplication of B. gibsoni was significantly inhibited by pyrimidine nucleotides such as cytidine 5'-monophosphate (5'-CMP), which is preferentially catalyzed by P5N-I and also inhibits the morphological maturation of canine reticulocytes. Purine nucleotides such as inosine 5'-monophospha te (5'-IMP) also had an inhibitory effect on the multiplication of this parasite. These results suggest that nucleotides such as 5'-CMP and 5'-IMP might accumulate in young erythrocytes and/or serum in dogs infected with B. gibsoni as a result of the decreased activity of erythrocyte 5'-nucleotidase, and the accumulation of these nucleotides might inhibit the multiplication of this parasite and simultaneously retard the maturation of reticulocytes. The results obtained from the in vitro examinations in the present study may partially clarify the relationship between low parasitemia and simultaneous reticulocytosis in vivo in canine babesiosis. KEY WORDS: Babesia gibsoni, erythrocyte 5'-nucleotidase, purine nucleotide, pyrimidine nucleotide, reticulocyte.

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“…It may be hypothesized that the chromatographic lead peak at 45 kDa reflects lead bound to pyrimidine-5-nucleotidase, an erythrocytic enzyme, which is inhibited by lead [13], requires magnesium for its activity, and has an apparent molecular mass of 45 kDa [14].…”

Section: Lead-binding Proteins In Human Erythrocytesmentioning

confidence: 99%

Lead-binding proteins - a way to understand lead toxicity?

Bergdahl¹

1998

Analusis

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Lead poisoning. Further observations on erythrocyte pyrimidine-nucleotidase deficiency and intracellular accumulation of pyrimidine nucleotides.

Cited by 57 publications

References 16 publications

Characterizing Golden Eagle Risk to Lead and Anticoagulant Rodenticide Exposure: A Review

Characterizing Golden Eagle Risk to Lead and Anticoagulant Rodenticide Exposure: A Review

Inhibitory Effect of Pyrimidine and Purine Nucleotides on the Multiplication of <i>Babesia gibsoni</i>: Possible Cause of Low Parasitemia and Simultaneous Reticulocytosis in Canine Babesiosis

Lead-binding proteins - a way to understand lead toxicity?

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