Lead (Pb) Accumulation in Human THP-1 Monocytes/Macrophages In Vitro and the Influence on Cell Apoptosis

Metryka, Emilia; Kupnicka, Patrycja; Kapczuk, Patrycja; Aszakiewicz, Beata; Piotrowska, Katarzyna; Tkacz, Marta; Gutowska, Izabela; Chlubek, Dariusz; Baranowska-Bosiacka, Irena

doi:10.1007/s12011-020-02215-7

Cited by 17 publications

(9 citation statements)

References 96 publications

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“…Our study revealed that Pb exposure greatly inhibited cell viability in PC12, while black soybean peptides can reduce the damage of lead exposure to cell viability. In the latest research on the effects of Pb accumulation on cell viability and apoptosis, it was shown that environmental exposure to low concentrations of Pb might greatly impair the function of macrophages, and an increase in the number of apoptotic cells may cause the occurrence of many diseases in the organism [ 28 ]. It has been found that when intracellular ROS increases, cell viability decreases, especially when the metal mixture is used as a promoter stimulus [ 29 ].…”

Section: Resultsmentioning

confidence: 99%

“…Yuan et al said SOD and GSH/GSSG quickly cleared the ROS within cells to keep the stability of the internal structures of the cells and tissue. Otherwise, high ROS levels will disrupt the cell balance [ 28 ].…”

Section: Resultsmentioning

confidence: 99%

“…Under normal physiological conditions, Nrf2, an essential transcription factor regulated by intracellular oxidative stress, and Keap1, a cytoplasmic chaperone protein molecule, bind to each other and maintain a relatively inhibited state [ 28 ]. The redox-sensitive repressor Keap1 mainly regulates of Nrf2 transcriptional activity.…”

Section: Resultsmentioning

confidence: 99%

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Alleviating Effects of Black Soybean Peptide on Oxidative Stress Injury Induced by Lead in PC12 Cells via Keap1/Nrf2/TXNIP Signaling Pathway

Wen

et al. 2022

Nutrients

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Many researchers have found that Pb exposure can cause oxidative stress damage to the body’s tissue. Black soybean peptide (BSP) has a variety of physiological functions, especially in terms of oxidative stress. Nevertheless, the mitigation function of BSPs on Pb-induced oxidative stress damage in PC12 cells has not been clearly defined. In this study, cell viability was detected by CCK8. Oxidative stress indicators, such as ROS, GSH/GSSG, MDA, SOD, CAT, GPx, and GR, were tested with biochemical kit. Protein expression of Keap1, Nrf2, and TXNIP was measured by Western blot. Compared with the control group, Pb reduced the cell viability of PC12 cells. However, BSP treatment significantly increased the viability of PC12 cells induced by lead exposure (p < 0.05). Lead can enrich the contents of MDA and ROS, but decrease the amount of CAT, SOD, GR, GPx, and GSH/GSSG in PC12 cells, while BSP can alleviate it (p < 0.05). Lead can enhance the expression of Keap1 and TXNIP proteins, but reduce Nrf2 expression. In contrast, BSPs reversed this phenomenon (p < 0.05). BSPs can alleviate oxidative stress injury induced by lead in PC12 cells through the Keap1/Nrf2/TXNIP signaling pathway.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Alleviating Effects of Black Soybean Peptide on Oxidative Stress Injury Induced by Lead in PC12 Cells via Keap1/Nrf2/TXNIP Signaling Pathway

Wen

et al. 2022

Nutrients

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“…Meanwhile, cadmium could also induce hepatocyte apoptosis and tissue damage through inflammatory reaction [ 89 ]. Even at very low lead concentrations, Pb was equally toxic to monocytes and macrophages, which shows a decline in cell viability and an increase in the number of apoptotic cells [ 90 ]. Zhou also found that lead could increase the expression of PPARγ and stimulate the cleavage of caspase-3 and PARP to induce cytotoxicity [ 91 ].…”

Section: Major Toxicity Mechanisms Induced By Mycotoxins and Heavy Me...mentioning

confidence: 99%

Detoxification of Selenium Yeast on Mycotoxins and Heavy Metals: a Review

Sun

Chen

Xiong

et al. 2023

Biol Trace Elem Res

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Mycotoxins are secondary metabolites produced by specific fungi. More than 400 different mycotoxins are known in the world, and the concentration of these toxins in food and feed often exceeds the acceptable limit, thus causing serious harm to animals and human body. At the same time, modern industrial agriculture will also bring a lot of environmental pollution in the development process, including the increase of heavy metal content, and often the clinical symptoms of low/medium level chronic heavy metal poisoning are not obvious, thus delaying the best treatment opportunity. However, the traditional ways of detoxification cannot completely eliminate the adverse effects of these toxins on the body, and sometimes bring some side effects, so it is essential to find a new type of safe antidote. Trace element selenium is among the essential mineral nutrient elements of human and animal bodies, which can effectively remove excessive free radicals and reactive oxygen species in the body, and has the effects of antioxidant, resisting stress, and improving body immunity. Selenium is common in nature in inorganic selenium and organic selenium. In previous studies, it was found that the use of inorganic selenium (sodium selenite) can play a certain protective role against mycotoxins and heavy metal poisoning. However, while it plays the role of antioxidant, it will also have adverse effects on the body. Therefore, it was found in the latest study that selenium yeast could not only replace the protective effect of sodium selenite on mycotoxins and heavy metal poisoning, but also improve the immunity of the body. Selenium yeast is an organic selenium source with high activity and low toxicity, which is produced by selenium relying on the cell protein structure of growing yeast. It not only has high absorption rate, but also can be stored in the body after meeting the physiological needs of the body for selenium, so as to avoid selenium deficiency again in the short term. However, few of these studies can clearly reveal the protective mechanism of yeast selenium. In this paper, the detoxification mechanism of selenium yeast on mycotoxins and heavy metal poisoning was reviewed, which provided some theoretical support for further understanding of the biological function of selenium yeast and its replacement for inorganic selenium. The conclusions suggest that selenium yeast can effectively alleviate the oxidative damage by regulating different signaling pathways, improving the activity of antioxidant enzymes, reversing the content of inflammatory factors, regulating the protein expression of apoptosis-related genes, and reducing the accumulation of mycotoxins and heavy metals in the body.

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“…These far exceeded the maximum permissible limit of 0.01 ppm (WHO) and 0.05 ppm (USEPA) for drinking water. The maximum permissible limit is of concern as Pb is a toxic metal that can cause impairment to nervous systems (particularly in children) which can result in brain and blood disorders (Metryka et al, 2021). Hematological damage is well noted with high Pb absorption, which is one of the most serious and important biochemical effects of Pb in drinking water (Ehi-Eromosele and Okiei, 2012).…”

Section: Heavy Metals Status In the Water And The Allowable Concentra...mentioning

confidence: 99%

The Consequence of Artisanal Gold Mining on Heavy Metals Exposure to Water in Anka, Zamfara State Nigeria

Yahaya

Abdu

Isa

2022

DASJ

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Over the decade many villages in Zamfara State Nigeria were known for artisanal gold mining which caused pollution of vast water bodies and area of land. This makes it imperative to analyze heavy metal contamination in wells and surface water. The present study evaluated the concentrations of heavy metals (iron, lead, cadmium, zinc, nickel, and chromium) in the ore processing water and well-water in some selected villages of Anka local government area in Zamfara State where mining is taking place. Anka town served as control due to absence of mining activities. The concentrations of heavy metals in the water samples were measured using atomic absorption spectrophotometry. The concentration of Fe, Pb, and Cd ranged from 23.3 – 921.46, 0 – 132.8, and 3.3 – 4.1 ppm respectively. While Zn, Ni, and Cr were not detected in the water samples. Virtually all the detected heavy metals in the water samples exceeded the international organizations (WHO, USEPA, and EPA-EUC) maximum permissible limit of 0.3 – 1.00, 0.01 – 0.05, and 0.03 – 0.05 ppm of Fe, Pb, and Cd in water samples respectively. Interestingly, all the well-water from all the study locations was not contaminated with Pb despite its high concentrations in the processing water. Meanwhile both the processing water and well water were contaminated with Fe and Cd. These revealed that artisanal gold mining is contributing to the pollution of surface water bodies (used for irrigation and drinking) with Pb, while Fe and Cd may be in addition to their abundance in natural soil deposits.

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Lead (Pb) Accumulation in Human THP-1 Monocytes/Macrophages In Vitro and the Influence on Cell Apoptosis

Cited by 17 publications

References 96 publications

Alleviating Effects of Black Soybean Peptide on Oxidative Stress Injury Induced by Lead in PC12 Cells via Keap1/Nrf2/TXNIP Signaling Pathway

Alleviating Effects of Black Soybean Peptide on Oxidative Stress Injury Induced by Lead in PC12 Cells via Keap1/Nrf2/TXNIP Signaling Pathway

Detoxification of Selenium Yeast on Mycotoxins and Heavy Metals: a Review

The Consequence of Artisanal Gold Mining on Heavy Metals Exposure to Water in Anka, Zamfara State Nigeria

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