Lead Halide Perovskite Nanocrystals–Phospholipid Micelles and Their Biological Applications: Multiplex Cellular Imaging and in Vitro Tumor Targeting

Abstract

Lead halide perovskite nanocrystals (NCs) are promising optical materials in many fields. However, their poor moisture stability, significant toxicity, and difficulty to be further functionalized greatly hinder their applications in bioimaging. Here, a universal strategy is demonstrated by simply encapsulating CsPbX3 (X = Cl, Br, I) NCs into phospholipids to achieve CsPbX3–phospholipid micelles (CsPbX3@phospholipid) as probes for multiplex encoding cellular imaging or tumor-targeted imaging. The layer of phospholipids endows CsPbX3 NCs with superior water-resistant characteristics, the ability to be further biofunctionalized, and greatly improved biocompatibility. The CsPbX3@phospholipid micelles exhibited strong luminescence with narrow fwhm in water for more than four months. Specifically, even after being modified with folic acid, the bright fluorescence of the micelles was well retained, which were employed for the targeting of Hela cells. Finally, the greatly reduced toxicity of the CsPbX3@phospholipid micelles was verified using HeLa cells and zebrafish as in vitro and in vivo models, respectively.

Stokes Shift in Inorganic Lead Halide Perovskites: Current Status and Perspective

“…[ 87 , 88 ] Through such system tumour detection by exosome imaging, [89] multiplex cellular imaging and tumour targeting has been reported. [90] …”

“…Inorganic metal halide based scintillators considered an auspicious approach due to fast response time without afterglow effect also having high spatial resolution providing better imaging and low cost radiographic opportunity with the presence of Stokes shift minimizing the signal cross talks along with enhancing the detection sensitivity [87,88] . Through such system tumour detection by exosome imaging, [89] multiplex cellular imaging and tumour targeting has been reported [90] …”

“…[87,88] Through such system tumour detection by exosome imaging, [89] multiplex cellular imaging and tumour targeting has been reported. [90] For display application, colour conversion materials with large Stokes shift are highly demanded, as the conventional luminescent materials without Stokes shift suffers from the poor contrast ratio exhibiting colourful appearance under environmental incident light. By making use of the Stokes shift, we may be able to improve the contrast ratio without additional filter for environmental light prevention.…”

Biocatalytic CsPbX3 Perovskite Nanocrystals: A Self‐Reporting Nanoprobe for Metabolism Analysis

“…PM-CsPbBr 3 NCs were prepared by a thin-film hydration method. [18] Briefly, CsPbBr 3 NCs and phospholipids were mixed in chloroform and gently dried in a nitrogen atmosphere (Figure 1a). After hydration by ultrasound for 10 s, the yellowish solution of PM-CsPbBr 3 NCs sample was obtained, showing bright green emission under 365 nm ultraviolet (UV) illumination (Figure 1c).…”

Ferroic alternation in methylammonium lead triiodide perovskite

“…Perovskite materials have received much attention for solar cells, 1,2 light-emitting diodes, 3,4 X-ray detectors, [5][6][7][8][9][10][11][12] β-ray scintillators, 13 artificial retinas, [14][15][16][17][18] intelligent nervous systems, 19 cell imaging, [20][21][22][23] piezoelectricity and pyroelectricity. 24,25 Organometal halide (OMH) perovskites are a family with a chemical formula of ABX 3 , where A is an organic group, B is Pb or Sn, and X is a halogen, I, Br, or Cl.…”