Abstract:The potential of allochthonous Bacillus sp. SKK11 and sesame oil cake extract for the immobilization of Pb in mine soil was investigated in this study. The isolate SKK11, isolated from a brackish environment and identified as Bacillus sp. based on partial 16S rDNA sequencing, exhibited maximum resistance to Pb (750 mg L À1 ). Growth kinetic studies revealed that the presence of oil cake extract (2%) increased the biomass of the isolate SKK11. Transmission electron microscopy and X-ray diffraction studies showe… Show more
“…A minor difference in the growth response was observed according to the metal. The differences in the growth profile could be associated with the toxicity and bioavailability of metals (Govarthanan et al 2015a). Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Several physical, chemical, and biological methods have been proposed for the removal of heavy metals from contaminated soil and water (Shi et al 2009), of which bacteria-based bioremediation is considered as a promising and viable method. Bacteria-based bioremediation is less expensive and eco-friendly (Govarthanan et al 2015a), but is highly influenced by several factors, such as survival of bacteria in contaminated soil or water, influence of abiotic factors on the growth of bacteria, mechanism of metal detoxification, expression rate of metal detoxificiation genes, and influence of pollutants on bacterial activity (Suja et al 2014; Govarthanan et al 2015b; Praburaman et al 2015). …”
The aim of the present study was to assess the bioremediation potential of endophytic bacteria isolated from roots of Tridax procumbens plant. Five bacterial endophytes were isolated and subsequently tested for minimal inhibitory concentration (MIC) against different heavy metals. Amongst the five isolates, strain RM exhibited the highest resistance to copper (750 mg/l), followed by zinc (500 mg/l), lead (450 mg/l), and arsenic (400 mg/l). Phylogenetic analysis of the 16S rDNA sequence suggested that strain RM was a member of genus Paneibacillus. Strain RM also had the capacity to produce secondary metabolites, indole acetic acid, siderophores, 1-aminocyclopropane-1-carboxylate (ACC) deaminase, and biosurfactant and solubilize phosphate. The growth kinetics of strain RM was altered slightly in the presence of metal stress. Temperature and pH influenced the metal removal rate. The results suggest that strain RM can survive under the high concentration of heavy metals and has been identified as a potential candidate for application in bioremediation of heavy metals in contaminated environments.
“…A minor difference in the growth response was observed according to the metal. The differences in the growth profile could be associated with the toxicity and bioavailability of metals (Govarthanan et al 2015a). Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Several physical, chemical, and biological methods have been proposed for the removal of heavy metals from contaminated soil and water (Shi et al 2009), of which bacteria-based bioremediation is considered as a promising and viable method. Bacteria-based bioremediation is less expensive and eco-friendly (Govarthanan et al 2015a), but is highly influenced by several factors, such as survival of bacteria in contaminated soil or water, influence of abiotic factors on the growth of bacteria, mechanism of metal detoxification, expression rate of metal detoxificiation genes, and influence of pollutants on bacterial activity (Suja et al 2014; Govarthanan et al 2015b; Praburaman et al 2015). …”
The aim of the present study was to assess the bioremediation potential of endophytic bacteria isolated from roots of Tridax procumbens plant. Five bacterial endophytes were isolated and subsequently tested for minimal inhibitory concentration (MIC) against different heavy metals. Amongst the five isolates, strain RM exhibited the highest resistance to copper (750 mg/l), followed by zinc (500 mg/l), lead (450 mg/l), and arsenic (400 mg/l). Phylogenetic analysis of the 16S rDNA sequence suggested that strain RM was a member of genus Paneibacillus. Strain RM also had the capacity to produce secondary metabolites, indole acetic acid, siderophores, 1-aminocyclopropane-1-carboxylate (ACC) deaminase, and biosurfactant and solubilize phosphate. The growth kinetics of strain RM was altered slightly in the presence of metal stress. Temperature and pH influenced the metal removal rate. The results suggest that strain RM can survive under the high concentration of heavy metals and has been identified as a potential candidate for application in bioremediation of heavy metals in contaminated environments.
“…Bacillus sp. SKK11 produce PbS crystals when cultured in LB medium ( 1 = 4 strength) supplemented with Pb(NO 3 ) 2 (Govarthanan et al 2015). Bacillus sp.…”
The aim of this study was to synthesize and characterize Cr(III) nanoparticles using wastewater from electroplating industries and chromium-resistant Bacillus subtilis. Formation of Cr(III) nanoparticles was confirmed by UV-visible (UV-Vis) spectroscopy at 300 nm. The size of the nanoparticles varied from 4 to 50 nm and energy dispersive spectroscopy profile shows strong Cr peak approximately at 4.45 and 5.2 keV. The nanoparticles inhibited the growth of pathogenic bacteria Staphylococcus aureus and Escherichia coli. The cytotoxic effect of the synthesized Cr(III) nanoparticle was studied using HEK 293 cells, and the cell viability was found to decrease with increasing concentration of Cr(III) nanoparticles.
“…The rich nutrients present in the SM had extended the log phase of growth kinetics. Our previous studies reported the nutrient properties of oil cakes and the growth profile of microorganisms [5][6][7]. The growth kinetics showed that the SM could be used as an alternative to the synthetic medium for the cultivation of bacteria.…”
Section: Growth Kinetics Of Rahnella Sp Rmmentioning
confidence: 99%
“…However, the disadvantages and ineffectiveness of these methods have been widely reported [2][3][4]. An alternative to the application of phyisco-chemical methods in heavy metal removal from contaminated soil/water is the use of microorganisms, capable of reduce, immobilize, transform, and leach the metal(loid)s [5][6][7].…”
Objective of the study was to isolate heavy metal resistant bacteria from chromiumcontaminated subsurface soil and investigate biosurfactant production and heavy metal bioremediation. Based on 16S rRNA gene sequence and phylogenetic analysis, the isolate was identified as Rahnella sp. RM. The biosurfactant production by heavy metal resistant Rahnella sp. RM was optimized using Box-Behnken design (BBD). The maximum emulsification activity was obtained 66 % at 6 % soybean meal in pH 7.0 and 33.5 °C. The biosurfactant was characterized using Field emission scanning electron microscopy (FE-SEM), Fourier transform infrared spectroscopy (FT-IR) and matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF). The highest metal removal rates using the biosurfactant were found 74.3, 72.5, and 70.1 %, respectively, at the 100 mg/L amended flasks at 48 h. This study indicated the biosurfactant from heavy metal resistant Rahnella sp. RM could be used as a potential tool to remediate the metals in contaminated environments.
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