Le contrôle de la synthèse protéique dans les cellules des eucaryotes

Houdebiné, Louis‐Marie

doi:10.1051/rnd:19810206

Cited by 2 publications

(1 citation statement)

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“…This provides more mRNA for polyribosome formation and leads directly to a stimulation of cardiac protein synthesis during the development of heart muscle hypertrophy. This agrees with the general concept that in most tissues cellular mRNA levels are rate-limiting for the cellular protein synthesis and that mRNA levels are controlled by activation/de-activation of transcription (11,37,44).…”

Section: Discussionsupporting

confidence: 85%

Influence of starvation and total protein deprivation on cardiac mRNA levels

et al. 1985

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The effect of starvation and of protein-deprivation on the extractable amount of cardiac mRNA was investigated in male rats. Cardiac mRNA was determined by either (a) isolation of cardiac mRNA by SDS-Phenol/oligo-dT-cellulose, or by (b) hybridization of cardiac mRNA to 3H-Poly(U). During starvation (1-6 days) the extractable amount of cardiac microsomal RNA decreased from 870 micrograms/g heart (controls) to 606 micrograms/g (3 days) and to 547 micrograms/g (6 days), the extractable amount of mRNA fell from 28.6 micrograms/g heart (controls) to 18.7 micrograms/g (3 days) and to 14.5 micrograms/g (6 days). When a normocaloric but protein-deficient diet was fed, the decreases in cardiac microsomal RNA and mRNA were qualitatively similar, but slightly less severe. An analysis of the intracellular distribution of cardiac microsomal RNA and mRNA in the hearts of normal animals and of animals starved or fed a protein-deficient diet indicates that during starvation cardiac mRNA does not accumulate in the cell sap, but gets rapidly degraded. In the refeeding period, mRNA is transported from the nucleus to the cytoplasm and engages in polyribosome formation. The specific mRNA species coding for the major myofibrillar cardiac proteins are affected to a similar extent by these changes during starvation/protein-deprivation and refeeding.

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Section: Discussionsupporting

confidence: 85%