The effect of triiodothyronine on the cardiac mRNA

Zähringer, J.; Klaubert, A

doi:10.1016/0022-2828(82)90143-2

Cited by 25 publications

(12 citation statements)

References 52 publications

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“…This shows that starvation/protein-deprivation does not alter the extractability of mRNA from heart muscle, but caused a true decrease in the cardiac mRNA content. Working with hyperthyroid animals, we have previously shown (44) that during induction of heart muscle hypertrophy the extractability of cardiac mRNA from rat heart muscle was also not affected. It therefore seems that changes in experimental conditions (atrophy or hypertrophy of heart muscle) do not significantly influence the extractability of cardiac mRNA from heart muscle.…”

Section: Effect Of Protein-deprivation On Cardiac Microsomal Rna and mentioning

confidence: 93%

“…Table 5 shows that after 3-6 days of starvation a significant decrease in the proteinsynthetic capacity of the isolated mRNA was observed. However, when the translational products synthesized in the reticulocyte lysate system under the direction of cardiac mRNA from normal or starved/protein-deficient animals are identified by separation on SDSPolyacrylamide-Slabgels and subsequent fluorography (40,42,44), no appreciable difference was seen in the pattern of the synthesized proteins encoded by the different mRNA preparations ( fig. 1), In each case, all major cardiac proteins (MHC, MLC1, and MLC2, actin, troponin, tropomyosin, myoglobin) were synthesized in approximately similar, relative ratios.…”

Section: In-vitro Translation Of Cardiac M R N a In A Cell-free Systemmentioning

confidence: 96%

“…muscle after starvation/protein-deprivation represents a true decrease in cardiac mRNA contents or whether it reflects only a decreased extractability of cardiac mRNA in the starved animals, we quantitated the cardiac mRNA content directly in heart homogenates by hybridization to 3H-Poly(U) as described in previous communications (43)(44)(45). Table 3 shows the results obtained and clearly demonstrates that in starved/proteindeficient rats the amount of cardiac Poly(A)-containing RNA hybridizable in heart homogenates to 3H-Poly(U) is greatly reduced.…”

Section: Effect Of Protein-deprivation On Cardiac Microsomal Rna and mentioning

confidence: 99%

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Influence of starvation and total protein deprivation on cardiac mRNA levels

et al. 1985

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The effect of starvation and of protein-deprivation on the extractable amount of cardiac mRNA was investigated in male rats. Cardiac mRNA was determined by either (a) isolation of cardiac mRNA by SDS-Phenol/oligo-dT-cellulose, or by (b) hybridization of cardiac mRNA to 3H-Poly(U). During starvation (1-6 days) the extractable amount of cardiac microsomal RNA decreased from 870 micrograms/g heart (controls) to 606 micrograms/g (3 days) and to 547 micrograms/g (6 days), the extractable amount of mRNA fell from 28.6 micrograms/g heart (controls) to 18.7 micrograms/g (3 days) and to 14.5 micrograms/g (6 days). When a normocaloric but protein-deficient diet was fed, the decreases in cardiac microsomal RNA and mRNA were qualitatively similar, but slightly less severe. An analysis of the intracellular distribution of cardiac microsomal RNA and mRNA in the hearts of normal animals and of animals starved or fed a protein-deficient diet indicates that during starvation cardiac mRNA does not accumulate in the cell sap, but gets rapidly degraded. In the refeeding period, mRNA is transported from the nucleus to the cytoplasm and engages in polyribosome formation. The specific mRNA species coding for the major myofibrillar cardiac proteins are affected to a similar extent by these changes during starvation/protein-deprivation and refeeding.

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Section: Effect Of Protein-deprivation On Cardiac Microsomal Rna and mentioning

confidence: 93%

Section: In-vitro Translation Of Cardiac M R N a In A Cell-free Systemmentioning

confidence: 96%

Section: Effect Of Protein-deprivation On Cardiac Microsomal Rna and mentioning

confidence: 99%

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Influence of starvation and total protein deprivation on cardiac mRNA levels

et al. 1985

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“…Recently, we have shown that intact cardiac Poly(A)-containing mRNA can be isolated from adult rat heart muscle (26) and that this mRNA can be hybridized to (3H)Poly(U) (25). These and similar techniques were employed by us and other authors in experimental animal studies investigating the pathogenesis of the adriamycin-cardiomyopathy (21,23), the triiodothyronine-induced heart hypertrophy (24), and also other forms of heart hypertrophy (5,12,18,22). For a more direct application of such investigations to human heart diseases it would be necessary to modify the techniques used in such a way that cardiac biopsies could serve as working material.…”

Section: Discussionmentioning

confidence: 94%

“…Using similar techniques, we have recently isolated intact Poly(A)-containing mRNA from adult rat cardiac muscle (26) and have determined the average sequence length of cardiac mRNA (2050 nucleotides) and of its Poly(A)-tract (88 nucleotides) (25). Subsequently, we have shown that changes in cardiac mRNA levels are involved in the 087 pathogenesis of the adriamycin-cardiomyopathy (21,23) and of the triiodothyronine-induced heart hypertrophy (24).…”

Section: Introductionmentioning

confidence: 97%

Quantitation of poly(A)-containing mRNA in rat cardiac biopsies

1983

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Using a combination of conventional mRNA-extraction methods and newer hybridization techniques, a method was developed which allows the quantitation of cardiac Poly(A)-containing mRNA in cardiac biopsies. This provides a new tool to directly assess changes in cardiac gene expression in cardiac biopsies. It is proposed that the method described may be valuable in investigations dealing with various human or animal heart diseases where changes in cardiac gene expression are expected and where only biopsy material is available.

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Control of protein synthesis and ribosome formation in rat heart

Russo

Morgan

1989

Diabetes Metab. Rev.

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The effect of triiodothyronine on the cardiac mRNA

Cited by 25 publications

References 52 publications

Influence of starvation and total protein deprivation on cardiac mRNA levels

Influence of starvation and total protein deprivation on cardiac mRNA levels

Quantitation of poly(A)-containing mRNA in rat cardiac biopsies

Control of protein synthesis and ribosome formation in rat heart

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