Le carcinome nasopharyngé en Afrique du Nord et dans le Sud-Est asiatique : entre similitude et différence

Ayadi, Wajdi; Khabir, Abdelmajid; Hadhri-Guiga, Boutheina; Fki, L.; Toumi, Nabil; Siala, W.; Fendri, Ali; Makni, H.; Boudawara, T.; Ghorbel, Abdelmonem; Gargouri, Ali; Jlidi, R.; Gargouri, R.; Busson, P.; Drira, M.; Daoud, J.; Frikha, M.; Hammami, Adnane; Karray-Hakim, Héla

doi:10.1684/bdc.2010.1090

Cited by 15 publications

(10 citation statements)

References 43 publications

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“…TSGs are suggested to play a key role in the transformation of nasopharyngeal epithelial cells into malignant ones [ 19 , 34 , 35 ]. Similarities of patterns in the chromosomal abnormalities have been reported in the NPC from different geographical regions [ 33 ]. Chromosome 3p21.3 was the first tumor suppressive region mapped in NPC [ 36 ].…”

Section: Discussionsupporting

confidence: 81%

“…The development of NPC involves the accumulation of multiple genetic changes affecting certain tumor suppressor genes (TSG) or oncogenes thus promoting the clonal evolution of the neoplastic cell, like in many other cancers [ 33 ]. TSGs are suggested to play a key role in the transformation of nasopharyngeal epithelial cells into malignant ones [ 19 , 34 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

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Integrin α9 gene promoter is hypermethylated and downregulated in nasopharyngeal carcinoma

Nawaz

et al. 2015

Oncotarget

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Epigenetic silencing of tumor suppressor genes (TSGs) by promoter methylation can be an early event in the multi-step process of carcinogenesis. Human chromosome 3 contains clusters of TSGs involved in many cancer types including nasopharyngeal carcinoma (NPC), the most common cancer in Southern China. Among ten candidate TSGs identified in chromosome 3 using NotI microarray, ITGA9 and WNT7A could be validated. 5′-aza-2′ deoxycytidine treatment restored the expression of ITGA9 and WNT7A in two NPC cell lines. Immunostaining showed strong expression of these genes in the membrane and cytoplasm of adjacent control nasopharyngeal epithelium cells, while they were weakly expressed in NPC tumor cells. The ITGA9 promoter showed marked differentially methylation between tumor and control tissue, whereas no differentially methylation could be detected for the WNT7A promoter. The expression level of ITGA9 in NPC tumors was downregulated 4.9-fold, compared to the expression in control. ITGA9 methylation was detected by methylation specific PCR (MSP) in 56% of EBV positive NPC- cases with 100% specificity. Taken together, this suggests that ITGA9 might be a TSG in NPC that is involved in tumor cell biology. The possibility of using ITGA9 methylation as a marker for early detection of NPC should further be explored.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Integrin α9 gene promoter is hypermethylated and downregulated in nasopharyngeal carcinoma

Nawaz

et al. 2015

Oncotarget

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“…Regardless of incidence and geographical distribution, its development has been attributed to an interaction of multiple factors: environmental including EBV infection and genetic factors [ 19 , 20 ]. Chromosomal abnormalities and aberrant promoter hypermethylation show a similar pattern in different geographical regions according to one study so far, supporting a common carcinogenic pathway to NPC irrespective of region [ 21 ].…”

Section: Introductionmentioning

confidence: 65%

Detection of nasopharyngeal carcinoma in Morocco (North Africa) using a multiplex methylation-specific PCR biomarker assay

et al. 2015

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BackgroundSilencing of tumor suppressor genes (TSGs) or activation of oncogenes by, e.g., aberrant promoter methylation, may be early events during carcinogenesis. The methylation status of such genes can be used for early detection of cancer. We are pursuing this approach in our efforts to develop markers for early detection and follow-up of nasopharyngeal carcinoma (NPC). We set out to develop this approach to allow identification of NPC from Morocco and then also compared with NPC samples from different geographical locations and different ethnicity with different NPC incidences, Epstein-Barr virus (EBV) prevalence, and environments.ResultsBy multiplex methylation-specific PCR (MMSP), multiple relevant genes can be detected simultaneously, to achieve high sensitivity and specificity. The strong association of EBV with NPC is also very useful in such an approach. We have initially screened for 12 potential marker genes including EBV genes coding for EBV nuclear antigen 1 (EBNA1) and latent membrane protein-1 (LMP1) and ten potential TSGs obtained from previously published data. The resulting assay included EBNA1, LMP1, and three cellular TSGs: ITGA9, RASSF1A, and P16. We evaluated this assay on 64 NPC patient biopsies from Morocco, Italy, and China compared to deoxyribonucleic acid (DNA) from 20 nasopharyngeal control tissues. In the Moroccan NPC cohort (n = 44), prevalence of the EBNA1 gene showed the highest sensitivity (36/44; 82 %) with 94 % specificity. Out of eight (18 %) EBNA1 negative Moroccan samples, only three were positive for at least one methylated cellular gene. By detection of cellular marker genes, the sensitivity increased from 82 to 89 % (39/44). In the whole material of 64 biopsies from three geographical locations, at least any one marker (viral or cellular) could be detected in 91 % of biopsies with 90 % specificity. In a pilot evaluating assay performance on serum DNA from NPC and controls including samples from Italy (n = 11) and China (n = 5), at least any one marker from the MMSP assay could be detected in 88 %, but the specificity was only 50 %.ConclusionsAn MMSP assay has the potential for detection of NPC by screening in high-risk populations. Serum-derived DNA seems not as good as earlier published NPC swab DNA for screening purpose.

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“…рака миндалины и лимфоэпителиального рака слюнных желез [31,32]; среди опухолей глотки -ВЭБ-ассоциированные случаи недифференцированного рака носоглотки [22,33]; среди опухолей желудка -ВЭБассоциированные случаи богатого лимфоидной инфильтрацией рака желудка [34]; среди злокачественных лимфом -лимфомы Ходжкина и Беркитта, а также целый спектр неходжкинских лимфом, возникших на фоне иммунодефицитных состояний [35,36]. Анализ показателей заболеваемости и смертности для указанных патологий в изучаемых нами регионах выявил следующее [37].…”

Section: экспериментальные статьиunclassified

Epstein–Barr virus in the ethnic Tatars population: the infection and sequence variants of LMP1 oncogene

et al. 2018

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Objective of the investigation was to study the infection of ethnic Tatars with the Epstein–Barr virus (EBV) and to analyze the genetic structure of the oncogene of the virus, the latent membrane protein 1 (LMP1), in the virus strains of Tatar origin. Materials and methods. The materials for the study were samples of boucle flushes of 60 students from the Kazan State Medical University who are ethnic Tatars (Tatars no less than in the 3rd generation). Amplified from DNA of boucle flushes the nucleotide sequences of the LMP1 samples translated into DNA amino acid sequences, have undergone classification based on the well-known and widely used in literature the R.H. Edwards et al. classification. Results. The analysis of nucleotide and deductive amino acid sequences of the 41 LMP1 amplicons revealed their homology with only three gene variants from the R.H. Edwards et al. classification (1999): 95.8/A (29.3 %; 12/41), Med– (14.6 %; 6/41) and China1 (7.3 %, 3/41). Such variants of LMP1 as Alaskan, Med+, Chinа2, China3 and NC, were not found. Among the LMP1 samples of Tatar origin in 20 cases (48.8 %), the composition of the mutations found did not allow them to be assigned to any of the oncogene variants listed above. Out of this number, in 7 (17.1 %) cases a mono group of LMP1 samples was found, differing not only from representatives of the Slavs, inhabitants of the European part of Russia, but also from other Kazan samples, and was designated as LMP1-TatK. The remaining 13 samples of LMP1 (31.7 %), not belonging to any of the known classifications, formed the group designated by us as an LMP1 group beside the classification (LMP1BC). Conclusion. Continuation of the study of the molecular-biological and functional properties of LMP1 in TatK and BC groups, which constitute 48.8 % of the number of gene samples studied, and an analysis of the peculiarities of the ethnic Tatar genotype, will probably help to clarify whether certain EBV strains influence morbidity and mortality in Tatar population with malignant neoplasms, which include EBVassociated cases.

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Le carcinome nasopharyngé en Afrique du Nord et dans le Sud-Est asiatique : entre similitude et différence

Cited by 15 publications

References 43 publications

Integrin α9 gene promoter is hypermethylated and downregulated in nasopharyngeal carcinoma

Integrin α9 gene promoter is hypermethylated and downregulated in nasopharyngeal carcinoma

Detection of nasopharyngeal carcinoma in Morocco (North Africa) using a multiplex methylation-specific PCR biomarker assay

Epstein–Barr virus in the ethnic Tatars population: the infection and sequence variants of LMP1 oncogene

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