Epstein–Barr virus in the ethnic Tatars population: the infection and sequence variants of LMP1 oncogene

Abstract

Objective of the investigation was to study the infection of ethnic Tatars with the Epstein–Barr virus (EBV) and to analyze the genetic structure of the oncogene of the virus, the latent membrane protein 1 (LMP1), in the virus strains of Tatar origin. Materials and methods. The materials for the study were samples of boucle flushes of 60 students from the Kazan State Medical University who are ethnic Tatars (Tatars no less than in the 3rd generation). Amplified from DNA of boucle flushes the nucleotide sequences of the LMP1 samples translated into DNA amino acid sequences, have undergone classification based on the well-known and widely used in literature the R.H. Edwards et al. classification. Results. The analysis of nucleotide and deductive amino acid sequences of the 41 LMP1 amplicons revealed their homology with only three gene variants from the R.H. Edwards et al. classification (1999): 95.8/A (29.3 %; 12/41), Med– (14.6 %; 6/41) and China1 (7.3 %, 3/41). Such variants of LMP1 as Alaskan, Med+, Chinа2, China3 and NC, were not found. Among the LMP1 samples of Tatar origin in 20 cases (48.8 %), the composition of the mutations found did not allow them to be assigned to any of the oncogene variants listed above. Out of this number, in 7 (17.1 %) cases a mono group of LMP1 samples was found, differing not only from representatives of the Slavs, inhabitants of the European part of Russia, but also from other Kazan samples, and was designated as LMP1-TatK. The remaining 13 samples of LMP1 (31.7 %), not belonging to any of the known classifications, formed the group designated by us as an LMP1 group beside the classification (LMP1BC). Conclusion. Continuation of the study of the molecular-biological and functional properties of LMP1 in TatK and BC groups, which constitute 48.8 % of the number of gene samples studied, and an analysis of the peculiarities of the ethnic Tatar genotype, will probably help to clarify whether certain EBV strains influence morbidity and mortality in Tatar population with malignant neoplasms, which include EBVassociated cases.

Scientific Substantiation of the Creation and Prospects for the Development of an Epidemiological Surveillance System for Infection Caused by the Epstein-Barr Virus

“…• биологическое разнообразие популяции возбу дителя -наличие двух генотипов и, по меньшей мере, семи фенотипов ВЭБ [9][10][11][12]…”

“…Установлено существование двух генотипов ВЭБ (ВЭБ 1 и ВЭБ 2) и восьми вариантов гена, кодирующего его латентный мембранный белок 1 (LMP1): B95.8, Alaskan (Ala), China 1(Ch1), China 2 (Ch2), China 3 (Ch3), Mediterranean+ (Med+), Mediterranean-(Med-) и North Carolina (NC) [9]. Имеются единичные сведения о распространен ности различных генотипов и вариантов ВЭБ среди населения Российской Федерации, не по зволяющие установить их однозначное влияние на заболеваемость как самой инфекцией, так и ас социированной с ней патологией [10][11][12].…”