LDL receptor gene transcription is selectively induced by t10c12-CLA but not by c9t11-CLA in the human hepatoma cell line HepG2

Ringseis, Robert; König, Bettina; Leuner, Beatrice; Schubert, Sandra; Naß, Norbert; Stangl, Gabriele I.; Eder, Klaus

doi:10.1016/j.bbalip.2006.08.007

Cited by 21 publications

(20 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our results are in agreement with other studies that have shown that dietary CLA can lower the serum cholesterol levels [30,37]. This decreased serum cholesterol and LDL levels by CLA rich soybean oil can be due to a possible transcriptional activation of the LDL receptor gene which in turn enhances the uptake of VLDL and LDL cholesterol via hepatic LDL receptors [38]. Other possible cholesterol lowering effects of CLA could be due to inhibition of secretion of apolipoprotein B or by inhibiting cholesterol absorption by down-regulating the intestinal sterol O-acyltransferase activity [39,40].…”

Section: Discussionsupporting

confidence: 95%

Trans–trans Conjugated Linoleic Acid Enriched Soybean Oil Reduces Fatty Liver and Lowers Serum Cholesterol in Obese Zucker Rats

Gilbert

Gadang

Proctor

et al. 2011

Lipids

View full text Add to dashboard Cite

Conjugated linoleic acid (CLA) is a collection of octadecadienoic fatty acids that have been shown to possess numerous health benefits. The CLA used in our study was produced by the photoisomerization of soybean oil and consists of about 20% CLA; this CLA consists of 75% trans-trans (a mixture of t8,t10; t9,t11; t10,t12) isomers. This method could be readily used to increase the CLA content of all soybean oil used as a food ingredient. The objective of this study was to determine the effects of trans-trans CLA-rich soy oil, fed as a dietary supplement, on body composition, dyslipidemia, hepatic steatosis, and markers of glucose control and liver function of obese fa/fa Zucker rats. The trans-trans CLA-rich soy oil lowered the serum cholesterol and low density lipoprotein-cholesterol levels by 41 and 50%, respectively, when compared to obese controls. Trans-trans CLA-rich soy oil supplementation also lowered the liver lipid content significantly (P < 0.05) with a concomitant decrease in the liver weight in the obese rats. In addition, glycated hemoglobin values were improved in the group receiving CLA-enriched soybean oil in comparison to the obese control. PPAR-γ expression in white adipose tissue was unchanged. In conclusion, trans-trans CLA-rich soy oil was effective in lowering total liver lipids and serum cholesterol.

show abstract

Section: Discussionsupporting

confidence: 95%

Trans–trans Conjugated Linoleic Acid Enriched Soybean Oil Reduces Fatty Liver and Lowers Serum Cholesterol in Obese Zucker Rats

Gilbert

Gadang

Proctor

et al. 2011

Lipids

View full text Add to dashboard Cite

show abstract

“…AB: statistical significance at P < 0.05. 5 -CACGGTCACCTGCTCCTG-3 (Dubuc et al, 2004;Ringseis et al, 2006).…”

Section: Ldl Receptor and Pcsk9 Mrna Expression Levelsmentioning

confidence: 99%

Phaleria macrocarpa (Scheff.) Boerl fruit aqueous extract enhances LDL receptor and PCSK9 expression in vivo and in vitro

Chong

Dollah

Chong

et al. 2011

Journal of Ethnopharmacology

View full text Add to dashboard Cite

show abstract

“…16 The cells were transiently cotransfected with 250 ng of pGL3 basic luciferase vector, pGL-762T or pGL-762C constructs, and 25 ng pRL-TK vector (Renilla luciferase-bearing vector) using jetPEI transfection reagent according to the manufacturer's instructions. In all transfections, pRL-TK vector (Renilla luciferase-bearing vector-Promega) served as an internal control to normalize the efficiency of transfection.…”

Section: Cell Culture and Transient Transfectionmentioning

confidence: 99%

The g.−762T>C polymorphism of the NPC1L1 gene is common in Chinese and contributes to a higher promoter activity and higher serum cholesterol levels

et al. 2009

View full text Add to dashboard Cite

Niemann-Pick type C1-like 1 (NPC1L1) protein is responsible for intestinal cholesterol absorption. The aim of the study was to identify genetic polymorphisms of the NPC1L1 gene as well as their functional significance. The method involved screening of promoter and coding regions of the NPC1L1 gene for genetic polymorphisms by direct DNA sequencing of genomic DNA from 50 individuals. Functional studies on promoter polymorphisms were performed using luciferase assay. Association between the polymorphisms and serum cholesterol levels were investigated in 224 individuals. The results showed that in total, 11 single nucleotide polymorphisms were identified. Among them, a promoter polymorphism, g.À762T4C, and a synonymous polymorphism, g.1679C4G, were common (34 and 36%, respectively). These two polymorphisms were highly linked (D¢ value¼0.7459, P-value o0.00001). For the g.À762T4C promoter polymorphism, luciferase assay in HepG2 cell line demonstrated that the À762C allele had a significantly higher promoter activity than the À762T allele (1.30 ± 0.22 vs 0.37 ± 0.06, 3.5-fold, Po0.05). We also showed that the NPC1L1 promoter activity was downregulated by cholesterol content in both genotypes. When association studies were performed, we found that À762C allele was associated with significantly higher serum total cholesterol and LDL-cholesterol content levels in a recessive model (LDL-cholesterol value¼131.2 ± 8.1 vs 116.4 ± 2.2 mg dl -1 ; total cholesterol value¼214.7 ± 9.0 mg dl -1 vs 196.9 ± 2.6, P-value o0.05, n¼224). In conclusion, the C allele at À762 position of the NPC1L1 gene was common in people of Chinese ethnicity. The À762C allele had a higher promoter activity and was associated with a higher serum total cholesterol and LDL-cholesterol level.

show abstract

LDL receptor gene transcription is selectively induced by t10c12-CLA but not by c9t11-CLA in the human hepatoma cell line HepG2

Cited by 21 publications

References 45 publications

Trans–trans Conjugated Linoleic Acid Enriched Soybean Oil Reduces Fatty Liver and Lowers Serum Cholesterol in Obese Zucker Rats

Trans–trans Conjugated Linoleic Acid Enriched Soybean Oil Reduces Fatty Liver and Lowers Serum Cholesterol in Obese Zucker Rats

Phaleria macrocarpa (Scheff.) Boerl fruit aqueous extract enhances LDL receptor and PCSK9 expression in vivo and in vitro

The g.−762T>C polymorphism of the NPC1L1 gene is common in Chinese and contributes to a higher promoter activity and higher serum cholesterol levels

Contact Info

Product

Resources

About