LcNAC13 Physically Interacts with LcR1MYB1 to Coregulate Anthocyanin Biosynthesis-Related Genes during Litchi Fruit Ripening

Jiang, Guoxiang; Li, Zhiwei; Song, Yunbo; Zhu, Hong; Lin, Sen; Huang, Riming; Jiang, Yueming; Duan, Xuewu

doi:10.3390/biom9040135

Cited by 39 publications

(21 citation statements)

References 58 publications

(83 reference statements)

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“…Anthocyanins are synthesized in cytosol, and stored in the vacuole. Studies on several plant species, including Arabidopsis ( Baudry et al, 2006 ; Gonzalez et al, 2008 ; Qi et al, 2011 ; Xie et al, 2016 ), agricultural crops ( Yang et al, 2019 ; Dong et al, 2020 ), fruits ( Rahim et al, 2014 ; Zhou et al, 2015 ; Jiang et al, 2019 ; Li C. et al, 2020 ), vegetable and ornamental plants ( Suzuki et al, 2016 ; Xu et al, 2017 ; Jin et al, 2018 , 2019 ; Zhu et al, 2019 ) have revealed that biosynthesis of anthocyanins are controlled by structural and regulatory genes that take part in formation as well as regulation of specific enzymes. The key enzymes including phenylalanine ammonia lyase (PAL), cinnamic acid 4-hydroxylase (C4H), 4-coumarate-CoA ligase (4CL), chalcone synthase (CHS), chalcone isomerase (CHI), flavonone 3-hydroxylase (F3H), dihydroflavonol 4-reductase (DFR), flavonoid 3′-monooxygenase (F3′H), anthocyanin synthase (ANS), as well as UDP-glucose-flavonoid 3- O -glucosyltrasnferase (UFGT) are important in anthocyanin biosynthesis ( Koes et al, 2005 ; Li et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, some other TFs such as the MYB-bHLH-WD (MBW) complex, B-box, bZIP, MYC, NAC, WRKY, bHLH, MADS-box, and WD could also coordinate anthocyanin biosynthesis initiation by binding to the promoter regions of structural genes ( Xu et al, 2015 ; Zhou et al, 2015 ; An et al, 2017 ; Lloyd et al, 2017 ; Lu et al, 2018 ; Fang et al, 2019 ; Jiang et al, 2019 ). For example, Arabidopsis bHLH TFs (GL3, TT8, and EGL3) and WD40 repeat protein TTG1 regulate anthocyanin biosynthetic gene expressions ( Gonzalez et al, 2008 ; Gerats and Strommer, 2009 ; Saito et al, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

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Comparative Transcriptome Analysis Identifies Key Regulatory Genes Involved in Anthocyanin Metabolism During Flower Development in Lycoris radiata

et al. 2021

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Lycoris is used as a garden flower due to the colorful and its special flowers. Floral coloration of Lycoris is a vital trait that is mainly regulated via the anthocyanin biosynthetic pathway. In this study, we performed a comparative transcriptome analysis of Lycoris radiata petals at four different flower development stages. A total of 38,798 differentially expressed genes (DEGs) were identified by RNA sequencing, and the correlation between the expression level of the DEGs and the anthocyanin content was explored. The identified DEGs are significantly categorized into ‘flavonoid biosynthesis,’ ‘phenylpropanoid biosynthesis,’ ‘Tropane, piperidine and pyridine alkaloid biosynthesis,’ ‘terpenoid backbone biosynthesis’ and ‘plant hormone signal transduction’ by Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. The candidate genes involved in anthocyanin accumulation in L. radiata petals during flower development stages were also identified, which included 56 structural genes (especially LrDFR1 and LrFLS) as well as 27 key transcription factor DEGs (such as C3H, GATA, MYB, and NAC). In addition, a key structural gene namely LrDFR1 of anthocyanin biosynthesis pathway was identified as a hub gene in anthocyanin metabolism network. During flower development stages, the expression level of LrDFR1 was positively correlated with the anthocyanin content. Subcellular localization revealed that LrDFR1 is majorly localized in the nucleus, cytoplasm and cell membrane. Overexpression of LrDFR1 increased the anthocyanin accumulation in tobacco leaves and Lycoris petals, suggesting that LrDFR1 acts as a positively regulator of anthocyanin biosynthesis. Our results provide new insights for elucidating the function of anthocyanins in L. radiata petal coloring during flower development.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptome Analysis Identifies Key Regulatory Genes Involved in Anthocyanin Metabolism During Flower Development in Lycoris radiata

et al. 2021

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“…MYB10, which belongs to Sg6, performs a key regulating function in anthocyanin biosynthesis through binding to the promoter of anthocyanin biosynthetic structural genes in Malus domestica, Pyrus spp., and Amygdalus persica (Espley et al, 2007;Feng et al, 2010;Zhou et al, 2019). Moreover, researchers have reported that other TFs, such as WRKY, ethylene response factors (ERF), NAC, zinc finger, and MADS-box proteins, are involved in the anthocyanin biosynthetic pathway in Pyrus spp., Litchi chinensis, Arabidopsis, and Brassica napus (Duan et al, 2018;Ni et al, 2018;Jiang et al, 2019). However, there has been little research addressing how genetic information and transcription contribute to the regulation of flower color in Malus spp.…”

Section: Introductionmentioning

confidence: 99%

How the Color Fades From Malus halliana Flowers: Transcriptome Sequencing and DNA Methylation Analysis

et al. 2020

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The flower color of many horticultural plants fades from red to white during the development stages, affecting ornamental value. We selected Malus halliana, a popular ornamental species, and analyzed the mechanisms of flower color fading using RNA sequencing. Forty-seven genes related to anthocyanin biosynthesis and two genes related to anthocyanin transport were identified; the expression of most of these genes declined dramatically with flower color fading, consistent with the change in the anthocyanin content. A number of transcription factors that might participate in anthocyanin biosynthesis were selected and analyzed. A phylogenetic tree was used to identify the key transcription factor. Using this approach, we identified MhMYB10 as directly regulating anthocyanin biosynthesis. MhMYB10 expression was strongly downregulated during flower development and was significantly positively related to the expression of anthocyanin biosynthetic genes and anthocyanin content in diverse varieties of Malus. To analyze the methylation level during flower development, the MhMYB10 promoter sequence was divided into 12 regions. The methylation levels of the R2 and R8 increased significantly as flower color faded and were inversely related to MhMYB10 expression and anthocyanin content. Therefore, we deduce that the increasing methylation activities of these two regions repressed MhMYB10 expression.

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“…LcR1MYB1 was also highly expressed during fruit ripening, moreover it can interact with LcNAC13 and weaken or even reverse the transcriptional inhibition of LcNAC13 on its target genes. This suggests that LcNAC13 and LcR1MYB1 may antagonistically regulate anthocyanin accumulation during litchi fruit ripening [126].…”

Section: Nac Tfs and Fruit Color Transformationmentioning

confidence: 93%

NAC Transcription Factor Family Regulation of Fruit Ripening and Quality: A Review

Liu

Grierson

et al. 2022

Cells

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The NAC transcription factor (TF) family is one of the largest plant-specific TF families and its members are involved in the regulation of many vital biological processes during plant growth and development. Recent studies have found that NAC TFs play important roles during the ripening of fleshy fruits and the development of quality attributes. This review focuses on the advances in our understanding of the function of NAC TFs in different fruits and their involvement in the biosynthesis and signal transduction of plant hormones, fruit textural changes, color transformation, accumulation of flavor compounds, seed development and fruit senescence. We discuss the theoretical basis and potential regulatory models for NAC TFs action and provide a comprehensive view of their multiple roles in modulating different aspects of fruit ripening and quality.

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LcNAC13 Physically Interacts with LcR1MYB1 to Coregulate Anthocyanin Biosynthesis-Related Genes during Litchi Fruit Ripening

Cited by 39 publications

References 58 publications

Comparative Transcriptome Analysis Identifies Key Regulatory Genes Involved in Anthocyanin Metabolism During Flower Development in Lycoris radiata

Comparative Transcriptome Analysis Identifies Key Regulatory Genes Involved in Anthocyanin Metabolism During Flower Development in Lycoris radiata

How the Color Fades From Malus halliana Flowers: Transcriptome Sequencing and DNA Methylation Analysis

NAC Transcription Factor Family Regulation of Fruit Ripening and Quality: A Review

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