LC/MS studies with moving belt interfaces

Games, David E.; Alcock, Nicholas J.; Cobelli, L.; Eckers, Christine; Games, Marguerite P. L.; Jones, Angela A.; Lant, M.S.; McDowall, Mark; Rossiter, M.; Smith, Roger; Westwood, Steven A.; Wong, H.-Y.

doi:10.1016/0020-7381(83)80083-7

Cited by 8 publications

(2 citation statements)

References 12 publications

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“…The origins of liquid chromatography mass spectrometry (LC–MS) can be traced back to 1968 where direct liquid introduction (DLI) from a capillary in to a high vacuum electron impact source of an MS instrument was demonstrated. Over the subsequent years (1973–1979), coupling LC to MS evolved through DLI and chemical ionization, − corona discharge in 1976, moving belt in 1976 and 1983, , fast atom bombardment in 1981, thermospray in 1978, and atmospheric pressure ionization in 1982 . One of the first demonstrations of LC-introduction (no rp-column) combined with the pneumatically assisted ion spray was in 1988 .…”

Section: A Brief History Of Denaturing and Native-ms And Its Early Ap...mentioning

confidence: 99%

Denaturing and Native Mass Spectrometric Analytics for Biotherapeutic Drug Discovery Research: Historical, Current, and Future Personal Perspectives

Campuzano

Sandoval²

2021

J. Am. Soc. Mass Spectrom.

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Mass spectrometry (MS) plays a key role throughout all stages of drug development and is now as ubiquitous as other analytical techniques such as surface plasmon resonance, nuclear magnetic resonance, and supercritical fluid chromatography, among others. Herein, we aim to discuss the history of MS, both electrospray and matrix-assisted laser desorption ionization, specifically for the analysis of antibodies, evolving through to denaturing and native-MS analysis of newer biologic moieties such as antibody–drug conjugates, multispecific antibodies, and interfering nucleic acid–based therapies. We discuss challenging therapeutic target characterization such as membrane protein receptors. Importantly, we compare and contrast the MS and hyphenated analytical chromatographic methods used to characterize these therapeutic modalities and targets within biopharmaceutical research and highlight the importance of appropriate MS deconvolution software and its essential contribution to project progression. Finally, we describe emerging applications and MS technologies that are still predominantly within either a development or academic stage of use but are poised to have significant impact on future drug development within the biopharmaceutic industry once matured. The views reflected herein are personal and are not meant to be an exhaustive list of all relevant MS performed within biopharmaceutical research but are what we feel have been historically, are currently, and will be in the future the most impactful for the drug development process.

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Section: A Brief History Of Denaturing and Native-ms And Its Early Ap...mentioning

confidence: 99%

Denaturing and Native Mass Spectrometric Analytics for Biotherapeutic Drug Discovery Research: Historical, Current, and Future Personal Perspectives

Campuzano

Sandoval²

2021

J. Am. Soc. Mass Spectrom.

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“…The application of denaturing reversed-phase liquid chromatography mass spectrometry (rpLC-MS) analyses and characterization of therapeutic proteins within biopharmaceutical research is as ubiquitous as the application of, but not limited to nuclear magnetic resonance (NMR), surface plasmon resonances (SPR) and calorimetry for biopharmaceutical project advancement and spans many if not all therapeutic modalities. , The origins of LC-MS can be traced back to 1968 when Tal’roze first coupled direct liquid introduction (DLI) from a capillary into a high-vacuum electron impact source of an MS instrument . Over the subsequent 15 years (1973–1988), coupling LC to MS evolved through DLI and chemical ionization, corona discharge, moving belt interface, fast atom bombardment, thermospray, and atmosphere pressure ionization . One of the first demonstrations of LC introduction (no RP column) combined with the pneumatically assisted ion spray was in 1988 where accurate molecular weight ( M w ) determination of melitin, horse heart myoglobin, and β-lactoglobulin was demonstrated.…”

Section: Introductionmentioning

confidence: 99%

High-Throughput Mass Spectrometry for Biopharma: A Universal Modality and Target Independent Analytical Method for Accurate Biomolecule Characterization

Campuzano

Pelegri-O’Day

Srinivasan

et al. 2022

J. Am. Soc. Mass Spectrom.

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Reversed-phase liquid chromatographic mass spectrometry (rpLC-MS) is a universal, platformed, and essential analytical technique within pharmaceutical and biopharmaceutical research. Typical rpLC method gradient times can range from 5 to 20 min. As monoclonal antibody (mAb) therapies continue to evolve and bispecific antibodies (BsAbs) become more established, research stage engineering panels will clearly evolve in size. Therefore, high-throughput (HT) MS and automated deconvolution methods are key for success. Additionally, newer therapeutics such as bispecific T-cell engagers and nucleic acid-based modalities will also require MS characterization. Herein, we present a modality and target agnostic HT solid-phase extraction (SPE) MS method that affords the analysis of a 96-well plate in 41.4 min, compared to the traditional rpLC-MS method that would typically take 14.4 h. The described method can accurately determine the molecular weights for monodispersed and highly polydispersed biotherapeutic species and membrane proteins; determine levels of glycosylation, glycation, and formylation; detect levels of chain mispairing; and determine accurate drug-to-antibody ratio values.

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Combined liquid chromatography mass spectrometry. Part I. Coupling by means of a moving belt interface

Arpino

1989

Mass Spectrometry Reviews

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LC/MS studies with moving belt interfaces

Cited by 8 publications

References 12 publications

Denaturing and Native Mass Spectrometric Analytics for Biotherapeutic Drug Discovery Research: Historical, Current, and Future Personal Perspectives

Denaturing and Native Mass Spectrometric Analytics for Biotherapeutic Drug Discovery Research: Historical, Current, and Future Personal Perspectives

High-Throughput Mass Spectrometry for Biopharma: A Universal Modality and Target Independent Analytical Method for Accurate Biomolecule Characterization

Combined liquid chromatography mass spectrometry. Part I. Coupling by means of a moving belt interface

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