LC-MS/MS simultaneous determination of 37 bioactive compounds in Bunium crassifolium Batt. and its biological activities

Abstract

Bunium crassifolium Batt. is an extremely rare species growing in Algeria. Its leaf or aerial part is used for flavoring and garnishing food. The aim of the current study was to determine 37 bioactive phytochemicals and biological evaluation of the methanol and methanol:water (70:30) extracts of B. crassifolium for the first time. A total of 10 phenolic acids and 8 flavonoids have been identified in the extracts of the aerial parts by LC-MS/MS. Chlorogenic, gallic and ferulic acids were the most abundant phenolic acids detected, while rhoifolin, quercitrin and rutin were the most abundant flavonoids. In addition, 3 non-phenolic organic acids (fumaric acids, quinic and malic) were detected and among them quinic and malic acids were the most abundant. Antioxidant activity was evaluted by six methods and the extracts showed significant activity. The total phenolic and flavonoid contents were determined and methanol:water (70:30) extract showed higher values which entails its greater antioxidant capacity. In addition, anticholinesterase (AChE and BChE) activity was evaluated for both extracts using Ellman method. Methanol extract showed a better anti-acetylcholinesterase activity than methanol:water (70:30) one. While, both extracts showed a weak anti-butyrylcholinesterase activity. Furthermore, the anti-tyrosinase activity was tested and the methanol:water (70:30) extract was promising and more potent. In conclusion, B. crassifolium could be used in food industries and pharmaceutical as a potential functional food ingredient.

Ethnobotanical investigation of Pistacia lentiscus L. grown in El Kala (Algeria), and phytochemical study and antioxidant activity of its essential oil and extracts

“…Before LC-MS/MS analysis, extracts of leaves, stems, and seeds were diluted to 1000 mg/L and filtered with a 0.2 µm syringe filter. A Nexera Shimadzu UHPLC model was used in conjunction with a tandem MS apparatus for a quantitative study of 37 phytochemicals was carried out (Souilah, Bendif, Ullah, HAMEL, et al 2021;Souilah, Bendif, Ullah, Miara, et al 2021). The chromatograph was equipped with a LC-30AD binary pump, a DGU-20A3R degasser, SIL-30AC automatic sampler and a CTO-10ASvp column oven.…”

“…Trolox was taken as a positive reference. The Ferrous ion chelating activity was determined according to (Souilah, Bendif, Ullah, HAMEL, et al 2021), and the results were expressed as IC 50 (mg/mL). While the Inhibition of β-carotene bleaching was carried out according to (Souilah, Bendif, Ullah, HAMEL, et al 2021).…”

“…The Ferrous ion chelating activity was determined according to (Souilah, Bendif, Ullah, HAMEL, et al 2021), and the results were expressed as IC 50 (mg/mL). While the Inhibition of β-carotene bleaching was carried out according to (Souilah, Bendif, Ullah, HAMEL, et al 2021). The results were expressed as IC 50 values, which are the concentration needed to prevent β-carotene bleaching by 50%.…”

Algerian Bunium incrassatum Seeds: Effects of Extraction Solvent Polarity on Phenolic Profile and Antioxidant Activity

“…But as usual for synthetic additives, BHA has been accepted as possibly carcinogenic to humans by the International Agency for Research on Cancer (IARC) due to the toxicological data on its mechanism of action (36). It should also be noted that all identified compounds have been reported previously in several studies performed on different species of Bunium (25,37,38), but none of them was about the seeds of B.incrassatum.…”

Determination of Fatty Acids Profile, Antioxidant, and Anticholinesterase Inhibitory Activities of Ganoderma lucidum collected from Swat, Pakistan

“…The inhibitory activities of acetylcholinesterase and butyrylcholinesterase were calculated by slight modification in the spectrophotometric method [34,37]. Acetylcholinesterase (AChE) from electric eel and Butyrylcholinesterase (BChE) from horse serum was used, while as substrates of the reaction, butyryl thiocholine chloride and acetylthiocholine iodide were employed.…”