LC-MS/MS Screening of Phenolic Compounds in Wild and Cultivated Grapes Vitis amurensis Rupr.

Abstract

This work represents a comparative metabolomic study of extracts of wild grapes obtained from six different places in the Primorsky and Khabarovsk territories (Far East Russia) and extracts of grapes obtained from the collection of N.I. Vavilov All-Russian Institute of Plant Genetic Resources (St. Petersburg). The metabolome analysis was performed by liquid chromatography in combination with ion trap mass spectrometry. The results showed the presence of 118 compounds in ethanolic extracts of V. amurensis grapes. In addition, several metabolites were newly annotated in V. amurensis. The highest diversity of phenolic compounds was identified in the samples of the V. amurensis grape collected in the vicinity of Vyazemsky (Khabarovsk Territory) and the floodplain of the Arsenyevka River (Primorsky Territory), compared to the other wild samples and cultural grapes obtained in the collection of N.I. Vavilov All-Russian Institute of Plant Genetic Resources.

Quality Characteristics and Anthocyanin Profiles of Different Vitis amurensis Grape Cultivars and Hybrids from Chinese Germplasm

“…Among the derivatives of different anthocyanidins, Mv-derivatives were the most abundant components in the three grape groups, which was in agreement with our previous research on V. vinifera cultivars [35] and East Asian species [18].The average percentages of Mv-derivatives in the skins of V. amurensis grapes (58%) and hybrids (56%) were significantly less than that in the three V. vinifera grape skins (80%) (Figure 4b), and was approximate for the average percentages of Pn-derivatives among the V. vinifera grapes (10%), V. amurensis grapes (8%) and hybrids (10%), while the average percentages of Dpderivatives, Cy-derivatives and Pt-derivatives in V. amurensis grapes (15%, 9% and 9%) and hybrids (19%, 6% and 9%) were obviously higher than those in the three V. vinifera grapes (4%, 1% and 5%). In addition, it has been confirmed that Pg-derivatives are present at trace concentrations in some grape cultivars of V. vinifera [36,37], V. amurensis [10,21], V. labrusca, V. aestivalis [18] and V. rotundifolia [38]. In this study, pg-derivatives were found in all the grapes assayed, accounting for 0.00046-0.10% of TA (Figure 4b).…”

“…Razgonova et al [10] identified 7 acylated derivatives, out of a total of 18 anthocyanin compounds, in V. amurensis grapes from Far East Russia. In this study, a richer group of acylated anthocyanin compounds (31) were detected (Table 2).…”

“…There are rich sources of phenolic compounds in the grapes of V. amurensis, including phenolic acids, stibenes, anthocynins, flavonols and flavan-3-ols [6][7][8][9]. In a recent study, a total of 118 phenolic metabolites were detected in wild and cultivated V. amurensis grapes collected from Far East Russia [10]. The phenolic extracts from V. amurensis have been proven to possess antioxidant [11], anti-inflammatory [12] and antiproliferative [13,14] activities, which could attenuate degenerative processes such as aging [15], cancer [16] and cardiovascular disease [17].…”

“…Among these phenolic compounds, the abundant content of anthocyanins is the most notable characteristic of V. amurensis [18]. The literature contains several reports concerning V. amurensis anthocyanins from studies using high-performance liquid chromatography coupled with mass detection (HPLC-MS) [8,10,[18][19][20][21];however, in these, the selected grape cultivars/accessions were neither comprehensive nor representative.…”

Exploring the Chemical Profiles and Biological Values of Two Spondias Species (S. dulcis and S. mombin): Valuable Sources of Bioactive Natural Products

“…MS/MS spectra of compounds produced fragment ion at m/z 304.054 [M-H-Hex], corresponding to taxifolin O -hexosides. Prominent fragment ions at m/z 259.061 [Agl-H-CO 2 ] − , 241.050 [Agl-H-CO 2 -H 2 O], and RDA ions at m/z 178.997 [ 1,2 A] − , 151.002 [ 1,3 A] − , 149.023 [ 1.3 B] − , and 125.023 [ 1,4 A] − confirmed the presence of taxifolin [ 36 ]. Based on the ratio between deprotonated molecular ion and fragments at m/z 304.054 [Y O - ] and 303.051 [Y O -H] − , the position of glycosylation of taxifolin can be distinguished ( Table 1 ).…”