LC–MS/MS for protein and peptide quantification in clinical chemistry

Evaluation of multiple reaction monitoring cubed for the analysis of tachykinin related peptides in rat spinal cord using a hybrid triple quadrupole-linear ion trap mass spectrometer

“…Each spiked sample was analyzed in six replicates including blanks along with standards prepared at concentrations ranging from 20 to 500 ng/mL to measure endogenous levels. In Table 2, the precision and accuracy data showed that both methods provided acceptable figures of merit with a marked better precision at the low concentrations in HPLC-MS/MRM 3 . As shown in Fig 2, interferences are present in HPLC-MS/MRM and it will inherently affect peak integration and consequently the precision of the method.…”

“…Moreover, neuropeptides can be used as biomarkers to support the development of new analgesic drugs and assist the decision making process in non-clinical drug development [1][2][3]. Mass spectrometry (MS) has a significant role in bioanalytical chemistry workflow.…”

Theory and Practice of UHPLC and UHPLC–MS

“…In addition, large pore size particles or monoliths are used to accommodate the size of proteins. These phases are particularly dominant in protein and peptide analysis, as shown in Rauh's review article on LCeMS/MS for protein and peptide quantification in clinical chemistry (Rauh, 2012). All examples shown in this article used C18 phases for a wide variety of proteins.…”

In situ androgen and estrogen biosynthesis in endometrial cancer: focus on androgen actions and intratumoral production

“…These results suggest that intratumoral estrogen metabolism and synthesis are pivotal in the progression of endometrial carcinoma, although the available data of sex-steroid hormone concentrations were all obtained by classical radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA) and not by the modern gold standard methods of mass spectrometry. This distinction may be important as the classical methods of RIA and ELISA have been the standard methodology to analyze steroid hormones; however, these methods harbor several problems, including sensitivity and specificity/cross-reactions (Rauh 2012). On the other hand, LC-MS/MS is highly specific and sensitive to validated methods, which could measure highly specific hormones from very small amounts of the sample (Rauh 2012).…”

“…This distinction may be important as the classical methods of RIA and ELISA have been the standard methodology to analyze steroid hormones; however, these methods harbor several problems, including sensitivity and specificity/cross-reactions (Rauh 2012). On the other hand, LC-MS/MS is highly specific and sensitive to validated methods, which could measure highly specific hormones from very small amounts of the sample (Rauh 2012).…”