Evaluation of multiple reaction monitoring cubed for the analysis of tachykinin related peptides in rat spinal cord using a hybrid triple quadrupole-linear ion trap mass spectrometer

Pailleux, Floriane; Beaudry, Francis

doi:10.1016/j.jchromb.2013.12.025

Cited by 8 publications

(2 citation statements)

References 10 publications

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“…This simple technique improves the specificity of measurements and allows quantifying targeted proteins in the low ng/mL range. The high specificity of this approach was further confirmed by quantification of tachykinin peptides, substance P (SP) and neurokinin A (NKA) by Pailleux et al [105].…”

Section: Novel Effective Strategies Based On Mrm/prm Acquisition Modesmentioning

confidence: 83%

Targeted liquid chromatography‐tandem mass spectrometry analysis of proteins: Basic principles, applications, and perspectives

2021

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Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) is now the main analytical method for the identification and quantification of peptides and proteins in biological samples. In modern research, identification of biomarkers and their quantitative comparison between samples are becoming increasingly important for discovery, validation, and monitoring. Such data can be obtained following specific signals after fragmentation of peptides using multiple reaction monitoring (MRM) and parallel reaction monitoring (PRM) methods, with high specificity, accuracy, and reproducibility. In addition, these methods allow measurement of the amount of posttranslationally modified forms and isoforms of proteins. This review article describes the basic principles of MRM assays, guidelines for sample preparation, recent advanced MRM-based strategies, applications and illustrative perspectives of MRM/PRM methods in clinical research and molecular biology.

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Section: Novel Effective Strategies Based On Mrm/prm Acquisition Modesmentioning

confidence: 83%

Targeted liquid chromatography‐tandem mass spectrometry analysis of proteins: Basic principles, applications, and perspectives

2021

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“…For example, Ranc et al (2012) utilized the extracted ion chromatograms to obtain relative quantities of different endogenous peptides in the tree shrew visual system. It should be noted that at the MS/MS level, multiple reaction monitoring, or monitoring of only specific, characteristic fragments, and PRM, or monitoring of all fragments, can lead to lowering the limit of detection by 100‐fold (Bobba et al, 2012; DeAtley et al, 2018; Pailleux & Beaudry, 2014; Saidi et al, 2019; Song & Liu, 2008; Wang et al, 2014; Yang et al, 2017).…”

Section: Advances In Quantitationmentioning

confidence: 99%

Recent advances in mass spectrometry analysis of neuropeptides

Phetsanthad

et al. 2021

Mass Spectrometry Reviews

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Due to their involvement in numerous biochemical pathways, neuropeptides have been the focus of many recent research studies. Unfortunately, classic analytical methods, such as western blots and enzyme‐linked immunosorbent assays, are extremely limited in terms of global investigations, leading researchers to search for more advanced techniques capable of probing the entire neuropeptidome of an organism. With recent technological advances, mass spectrometry (MS) has provided methodology to gain global knowledge of a neuropeptidome on a spatial, temporal, and quantitative level. This review will cover key considerations for the analysis of neuropeptides by MS, including sample preparation strategies, instrumental advances for identification, structural characterization, and imaging; insightful functional studies; and newly developed absolute and relative quantitation strategies. While many discoveries have been made with MS, the methodology is still in its infancy. Many of the current challenges and areas that need development will also be highlighted in this review.

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MRM3-based LC-MS multi-method for the detection and quantification of nut allergens

Korte

Brockmeyer

2016

Anal Bioanal Chem

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Food allergies have become a global challenge to food safety in industrialized countries in recent years. With governmental monitoring and legislation moving towards the establishment of threshold allergen doses, there is a need for sensitive and quantitative analytical methods for the determination of allergenic food contaminants. Targeted proteomics employing liquid chromatography-mass spectrometry (LC-MS) has emerged as a promising technique that offers increased specificity and reproducibility compared to antibody and DNA-based technologies. As the detection of trace levels of allergenic food contaminants also demands excellent sensitivity, we aimed to significantly increase the analytical performance of LC-MS by utilizing multiple reaction monitoring cubed (MRM) technology. Following a bottom-up proteomics approach, including a straightforward sample preparation process, 38 MRM experiments specific to 18 proteotypic peptides were developed and optimized. This permitted the highly specific identification of peanut, almond, cashew, hazelnut, pistachio, and walnut. The analytical performance of the method was assessed for three relevant food matrices with different chemical compositions. Limits of detection were around 1 μg/g or below in fortified matrix samples, not accounting for the effects of food processing. Compared to an MRM-based approach, the MRM-based method showed an increase in sensitivity of up to 30-fold. Regression analysis demonstrated high linearity of the MRM signal in spiked matrix samples together with robust intersample reproducibility, confirming that the method is highly applicable for quantitative purposes. To the best of our knowledge, we describe here the most sensitive LC-MS multi-method for food allergen detection thus far. In addition, this is the first study that systematically compares MRM with MRM for the analysis of complex foods. Graphical abstract Allergen detection by MRM.

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Evaluation of multiple reaction monitoring cubed for the analysis of tachykinin related peptides in rat spinal cord using a hybrid triple quadrupole-linear ion trap mass spectrometer

Abstract: improve the assay sensitivity to detect difference between samples by reducing significantly the potential of interferences and therefore reduce instrumental errors.

Cited by 8 publications

References 10 publications

Targeted liquid chromatography‐tandem mass spectrometry analysis of proteins: Basic principles, applications, and perspectives

Targeted liquid chromatography‐tandem mass spectrometry analysis of proteins: Basic principles, applications, and perspectives

Recent advances in mass spectrometry analysis of neuropeptides

MRM3-based LC-MS multi-method for the detection and quantification of nut allergens

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