LC–MS/MS determination and pharmacokinetic study of seven flavonoids in rat plasma after oral administration of Cirsium japonicum DC. extract

Zhang, Zhiyong; Jia, Peipei; Zhang, Xiaoxu; Zhang, Qiaoyue; Huang, Yang; He, Shan; Zhang, Lantong

doi:10.1016/j.jep.2014.10.022

Cited by 65 publications

(42 citation statements)

References 13 publications

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“…Previous studies conducted regular LC–MS/MS analysis to investigate the pharmacokinetics of umbelliferone, apigenin, genkwanin and hydroxygenkwanin in rats after administration of plant extracts (Song, Zhang, Liu, & Jin, ; Sun et al, ; Wei et al, ; Zhang et al, ; Zhao et al, ; Zheng, Yin, Xu, & Wang, ). Thus far, no reports are available on simultaneous determination and comprehensive pharmacokinetic studies of these four compounds.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of three flavonoids and one coumarin by LC–MS/MS: Application to a comparative pharmacokinetic study in normal and arthritic rats after oral administration of Daphne genkwa extract

Zhou

Yan

2018

Biomedical Chromatography

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A selective and sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for investigating the pharmacokinetics of umbelliferone, apigenin, genkwanin and hydroxygenkwanin after oral administration of Daphne genkwa extract. Plasma samples were treated by protein precipitation with acetonitrile. Analytes were detected by triple-quadrupole MS/MS with an ESI source in negative selection reaction monitoring mode. The transitions of m/z 161 → 133 for umbelliferone, m/z 269 → 117 for apigenin, m/z 283 → 268 for genkwanin and m/z 299 → 284 for hydroxygenkwanin were confirmed for quantification. Chromatographic separation was conducted using an Eclipse XDB-C column, and the applied isocratic elution program allowed for simultaneous determination of the four analytes for a total run time of 2.5 min. The linearity was validated over the plasma concentration ranges of 1.421-1421 ng/mL for umbelliferone, 0.845-845 ng/mL for apigenin, 1.025-1025 ng/mL for genkwanin and 0.845-845 ng/mL for hydroxygenkwanin. The extraction recovery rate was >82.7% for each analyte. No apparent matrix effect was observed during the bioanalysis. After full validation, the proposed method was successfully applied to compare the pharmacokinetics of these analytes between normal and arthritic rats.

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Section: Introductionmentioning

confidence: 99%

Simultaneous determination of three flavonoids and one coumarin by LC–MS/MS: Application to a comparative pharmacokinetic study in normal and arthritic rats after oral administration of Daphne genkwa extract

Zhou

Yan

2018

Biomedical Chromatography

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“…Usually, adding water miscible organic solvent can make intramolecular and intermolecular hydrogen bonding of protein change so that the drug with protein-bound fraction can be released [43][44][45][46]. The principle is based on the following aspects: (1) the hydrophilic organic solvent introduced as protein binding releasing agent can reduce the dielectric constant of the sample solution, resulting in enhanced electrostatic attraction between solute molecules; (2) the competitive binding for water between hydrosoluble organic solvent and protein can directly damage the protein surface hydration layer, make the protein spatial structure expand and release drug from drug-bound fraction [47].…”

Section: Selection Of Protein Binding Releasing Agentmentioning

confidence: 99%

“…PPT, a traditional pretreatment, is usually introduced for total concentration determination [45,46]. However, the sensitivity is usually reduced by several folds due to the addition of organic precipitant.…”

Section: Estimation and Comparison Of Hfcf-uf With Pptmentioning

confidence: 99%

Pretreatment of plasma samples by a novel hollow fiber centrifugal ultrafiltration technique for the determination of plasma protein binding of three coumarins using acetone as protein binding releasing agent

Shi

Jiang

et al. 2015

Journal of Chromatography B

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“…Several methods based on HPLC‐UV (Jia et al ., ) and LC‐MS/MS (Yan et al ., ; Zhang et al ., ; Guo et al ., ) have been reported for the determination of linarin in biological samples. Jia et al .…”

Section: Introductionmentioning

confidence: 97%

“…Several methods based on HPLC-UV ( Jia et al, 2006) and LC-MS/ MS (Yan et al, 2013;Zhang et al, 2014;Guo et al, 2015) have been reported for the determination of linarin in biological samples. Jia et al (2006) reported the first HPLC-UV method for quantifying linarin in beagle dog plasma.…”

Section: Introductionmentioning

confidence: 99%

A rapid and sensitive LC‐MS/MS method for the determination of linarin in small‐volume rat plasma and tissue samples and its application to pharmacokinetic and tissue distribution study

Feng

Liu

Wang

et al. 2015

Biomedical Chromatography

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A rapid and sensitive LC-MS/MS method was developed for the determination of linarin in small-volume rat plasma and tissue sample. Sample preparation was employed by the combination of protein precipitation (PPT) and liquid-liquid extraction (LLE) to allow measurement over a 5-order-of-magnitude concentration range. Fast chromatographic separation was achieved on a Hypersil Gold column (100 × 2.1 mm i.d., 5 µm). Mass spectrometric detection was achieved using a triple-quadrupole mass spectrometer equipped with an electrospray ionization interface operating in positive ionization mode. Quantification was performed using selected reaction monitoring of precursor-product ion transitions at m/z 593 → 285 for linarin and m/z 447 → 271 for baicalin (internal standard). The total run time was only 2.8 min per sample. The calibration curves were linear over the concentration range of 0.4-200 µg/mL for PPT and 0.001-1.0 µg/mL for LLE. A lower limit of quantification of 1.0 ng/mL was achieved using only 20 μL of plasma or tissue homogenate. The intra- and inter-day precisions in all samples were ≤14.7%, while the accuracy was within ±5.2% of nominal values. The validated method has been successfully applied to pharmacokinetic and tissue distribution study of linarin.

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LC–MS/MS determination and pharmacokinetic study of seven flavonoids in rat plasma after oral administration of Cirsium japonicum DC. extract

Cited by 65 publications

References 13 publications

Simultaneous determination of three flavonoids and one coumarin by LC–MS/MS: Application to a comparative pharmacokinetic study in normal and arthritic rats after oral administration of Daphne genkwa extract

Simultaneous determination of three flavonoids and one coumarin by LC–MS/MS: Application to a comparative pharmacokinetic study in normal and arthritic rats after oral administration of Daphne genkwa extract

Pretreatment of plasma samples by a novel hollow fiber centrifugal ultrafiltration technique for the determination of plasma protein binding of three coumarins using acetone as protein binding releasing agent

A rapid and sensitive LC‐MS/MS method for the determination of linarin in small‐volume rat plasma and tissue samples and its application to pharmacokinetic and tissue distribution study

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