LC‐MS/MS determination and pharmacokinetic study of albiflorin and paeoniflorin in rat plasma after oral administration of Radix Paeoniae Alba extract and Tang‐Min‐Ling‐Wan

Abstract

A rapid, sensitive and selective liquid chromatography/tandem mass spectrometry method (LC-MS/MS) was developed and validated for simultaneous determination of albiflorin and paeoniflorin in rat plasma using geniposide as an internal standard. Plasma samples were extracted by solid-phase extraction. Chromatographic separation was carried out on a Zorbax SB-C(18) analytical column (150 × 2.1 mm × 5 µm) with 0.1% formic acid-acetonitrile (70:30, v/v) as the mobile phase. Detection was performed by multiple reaction monitoring mode using electrospray ionization in the positive ion mode. The total run time was 3.0 min between injections. The calibration curves were linear over a range of 1-1000 ng/mL for albiflorin and 2-2000 ng/mL for paeoniflorin. The overall precision and accuracy for all concentrations of quality controls and standards were better than 15%. Mean recovery was determined to be 87.7% for albiflorin and 88.8% for paeoniflorin. The validated method was successfully applied to the pharmacokinetic study of albiflorin and paeoniflorin in rat plasma after oral administration of Radix Paeoniae Alba extract and Tang-Min-Ling-Wan. The pharmacokinetic parameters showed that albiflorin and paeoniflorin from Tang-Min-Ling-Wan were absorbed more rapidly with higher concentrations in plasma than that from Radix Paeoniae Alba extract. The results provided a meaningful basis for evaluating the clinical applications of traditional Chinese medicine.

Food- and gender-dependent pharmacokinetics of paeoniflorin after oral administration with Samul-tang in rats

“…Commonly, most pharmacokinetics of single herbal extracts as well as prescriptions or formulas show relatively a greater variation of plasma concentration and pharmacokinetic parameters than those of single compound, because they may contain multiple bio-active components to affect pharmacokinetic parameters. This phenomenon of paeoniflorin is consistent with other reports (Yang et al, 2006;Tong et al, 2010;Wu et al, 2009;Liu et al, 2011). The pharmacokinetic parameters of paeoniflorin are summarized in Table 2 observed between the male and female rats.…”

“…In pharmacokinetic study, one or more active compounds as the targeted components are selected to represent the pharmacokinetic of entire extract and prescription due to the existence of too complex ingredients in traditional herbal medicines (Wu et al, 2009). Paeoniflorin is a major bio-active component in paeoniflorin-containing herbal medicines and their prescription such as Cortex Moutan, Radix Paeoniae, Samul-tang, Shaoyao-Gancao-Tang, Shuang-Dan and Tang-Min-Ling-wan and used as a representative compound in their pharmacokinetic study (Gan et al, 2012;Tong et al, 2010;Wu et al, 2009). In this respect, we selected paeoniflorin as a target compound.…”

“…In this regards, paeoniflorin was selected as an indicator in Samul-tang for pharmacokinetic study. Each pharmacokinetic study of major active constituents, single herbs and formulas in traditional herbal medicine are usually considered to be essential because the biological effects of active constituents in medicinal herbs could be affected in the relation with different prescriptions (Tong et al, 2010).…”

Simultaneous determination of ten compounds in rat plasma by UPLC-MS/MS: Application in the pharmacokinetic study of Ma-Zi-Ren-Wan

“…Up to now, several studies have reported the pharmacokinetics of some of these ten compounds in other Chinese formula or single herbs [11][12][13][14][15][16][17][18][19][20][21][22][23][24]. However, the possibility of interaction between components in an herbal formula and the Fig.…”

Pharmacokinetics and disposition of monoterpene glycosides derived from Paeonia lactiflora roots (Chishao) after intravenous dosing of antiseptic XueBiJing injection in human subjects and rats

“…Cell-and animal-based studies have shown that paeoniflorin and other monoterpene glycosides exhibit anti-inflammatory, antiplatelet, antithrombosis, antioxidative, and neuroprotective properties [19][20][21][22][23][24][25][26][27][28][29][30][31][32][33] . Due to these pharmacological properties, several bioanalytical assays were developed to measure plasma concentrations of paeoniflorin and albiflorin [34][35][36] . However, PK data regarding the monoterpene glycosides remain quite limited, particularly human PK data.…”