LC–MS/MS determination and pharmacokinetic study of five flavone components after solvent extraction/acid hydrolysis in rat plasma after oral administration of Verbena officinalis L. extract

Duan, Kunfeng; Yuan, Zhifang; Guo, Wei; Yuan, Meng; Yang, Chao; Kong, Dezhi; Zhang, Lantong; Wang, Na

doi:10.1016/j.jep.2011.01.002

Cited by 58 publications

(34 citation statements)

References 10 publications

(7 reference statements)

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“…The plasma concentration-time curves of flavonoid aglycones after oral administration showed a double peak or shoulder, which was also found in the published papers (Duan et al, 2011;He et al, 2013). The underlying mechanism of this effect remains unknown, but it was demonstrated that both the contribution of hepatic metabolism and intestinal metabolism have been recognized as clinically important factors in the pharmacokinetics of orally administered drugs (Emoto et al, 2010;Wu et al, 1995).…”

Section: Double Peak Phenomenonmentioning

confidence: 54%

Interactions of pharmacokinetic profile of different parts from Ginkgo biloba extract in rats

Guan

Qian

Ren

et al. 2014

Journal of Ethnopharmacology

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Section: Double Peak Phenomenonmentioning

confidence: 54%

Interactions of pharmacokinetic profile of different parts from Ginkgo biloba extract in rats

Guan

Qian

Ren

et al. 2014

Journal of Ethnopharmacology

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“…A number of factors leading to the atypical drug absorption profiles include enterohepatic recirculation, variable gastric emptying and reabsorbed after tissue distribution (Duan etal. , ; Lin, Li, Wu, Kuo, & Tsai, ; Metsugi, Miyaji, Ogawara, Higaki, & Kimura, ). Of course, more detailed studies should be further carried out to investigate the double peaks mechanisms such as comparison of AUC 0– t obtained after oral intake of the compound in normal and bile duct cannulated rats (Zhang etal.…”

Section: Discussionmentioning

confidence: 99%

“…It is well known that drug absorption is a very complex process that manifests itself through potential interaction with a host of physicochemical and physiological variables (Macheras & Argyrakis, 1997;Zhou, 2003). A number of factors leading to the atypical drug absorption profiles include enterohepatic recirculation, variable gastric emptying and reabsorbed after tissue distribution (Duan et al, 2011;Lin, Li, Wu, Kuo, & Tsai, 2012;Metsugi, Miyaji, Ogawara, Higaki, & Kimura, 2008). Of course, more detailed studies should be further carried out to investigate the double peaks mechanisms such as comparison of AUC 0-t obtained after oral intake of the compound in normal and bile duct cannulated rats (Zhang et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Development and validation of an UPLC‐Q/TOF‐MS assay for the quantitation of neopanaxadiol in beagle dog plasma: Application to a pharmacokinetic study

Geng

Wang

et al. 2016

Biomedical Chromatography

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Neopanaxadiol (NPD), the main panaxadiol constituent of Panax ginseng C. A. Meyer (Araliaceae), has been regarded as the active component for the treatment of Alzheimer's disease. However, few references are available about pharmacokinetic evaluation for NPD. Accordingly, a rapid and sensitive method for quantitative analysis of NPD in beagle dog plasma based on ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry was developed and validated. Analytes were extracted from plasma by liquid-liquid extraction and chromatographic separation was achieved on an Agilent Zorbax Stable Bond C column. Detection was performed in the positive ion mode using multiple reaction monitoring of the transitions both at m/z 461.4 → 425.4 for NPD and internal standard of panaxadiol. All validation parameters, such as lower limit of quantitation, linearity, specificity, precision, accuracy, extraction recovery, matrix effect and stability, were within acceptable ranges and the method was appropriate for multitude sample determination. After oral intake, NPD was slowly absorbed and eliminated from circulatory blood system and corresponding plasma exposure was low. Application of this quantitative method will yield the first pharmacokinetic profile after oral administration of NPD to beagle dog. The information obtained here will be useful to understand the pharmacological effects of NPD.

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“…The system (ALS 1290 series; USA) consist of binary pump, vacuum degasser and auto-sampler system connected electro spray ionization (ESI) source). Samples obtained from Franz diffusion cell were injected, using acetonitrile and 0.1% formic acid in water (50:50, v/v) pumped at a constant flow of 0.3 ml/min (Duan et al, 2011;He et al, 2013). The standard graphs for the pure components (Quercetin, Kaempferol and Isorhamnetine) were made within the range of 2.5 -15mg/ml for each component.…”

Section: Release Kinetics: Lc-ms Methodsmentioning

confidence: 99%

Development, characterization and cytotoxicity evaluation of Gingko biloba extract (EGB761) loaded microemulsion for intra-nasal application

Singh¹,

Singh²,

Rachana³

2017

J App Pharm Sci

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Background: Ginkgo biloba is therapeutically important fossil species with extended benefits against CNS disorders as; it contains various potential constituents which are known for their specific actions. In spite of this fact its medicinal usage are limited due to the limited absorption, rapid elimination, vigorous biotransformation and low bioavailability of its therapeutic components. Objective: The present study is an attempt to develop a microemulsion system of standardized extract of Ginkgo biloba (EGB761) for intranasal application. Material and methods: It is developed by water titration method using Isopropyl Myristate (oil), Tween 80 (surfactant) and Ethanol (co surfactant). Results: EGB761 loaded microemulsions were developed and characterized. The optimized formulation showed particle size of 259.8 ± 6.3 nm, PDI score of 0.186 ± 0.092 and zeta potential as -9.87 ± 2.23 mv. Also, TEM images showed size range from 84 -260 nm with smooth and spherical morphology. In vitro phyto-constituents release through isolated nasal mucosa showed release pattern of zero order kinetics with sustained release (up to 16 hours). Moreover, cytotoxicity evaluation (RPMI2650 cell lines) didn't show cytotoxicity for both GBME (99.3 % ± 2.28 % and 98.17 % ± 1.86%) and plain EGB761 extract (95.3 % ± 2.1 and 91.7 % ± 1.07) with different time periods (3 and 12 hours). Conclusion: Therefore, it has been concluded from the results that, EGB761 based microemulsion (GBME) is developed with particle size range between 84 -260 nm, having zero order release kinetics. GBME is found to be safe on RPMI2650 cell line and can be further, investigated in in vivo system.

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LC–MS/MS determination and pharmacokinetic study of five flavone components after solvent extraction/acid hydrolysis in rat plasma after oral administration of Verbena officinalis L. extract

Cited by 58 publications

References 10 publications

Interactions of pharmacokinetic profile of different parts from Ginkgo biloba extract in rats

Interactions of pharmacokinetic profile of different parts from Ginkgo biloba extract in rats

Development and validation of an UPLC‐Q/TOF‐MS assay for the quantitation of neopanaxadiol in beagle dog plasma: Application to a pharmacokinetic study

Development, characterization and cytotoxicity evaluation of Gingko biloba extract (EGB761) loaded microemulsion for intra-nasal application

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