LC–MS/MS coupled with QSAR modeling in characterising of angiotensin I-converting enzyme inhibitory peptides from soybean proteins

ACE Inhibitory and Antioxidant Activities of Novel Peptides from Scorpaena notata By-product Protein Hydrolysate

“…This suggested the hydrolysis of the potent peptide leading to the release of molecules with a smaller size and higher bioactivity. Our results were similar to those of Fujita and Yoshikawa (1999) and Gu and Wu (2013) who reported a highest ACE inhibitory activity after digestion with gastrointestinal proteases. However, other studies showed no apparent change after incubation with gastrointestinal proteases (Balti et al 2010a, b), that can be due to the short sequence of the peptides.…”

“…Many highly effective ACE inhibitory peptides reported before contain hydrophobic amino acids (aromatic or with branched side chains) or the iminoacid proline at the C-terminal and branched-chain aliphatic amino acids at the N-terminal (Byun and Kim 2001;Wijesekara and Kim 2010). Besides, isoleucine and valine are frequently observed in potent ACE inhibitory peptides (Gu and Wu 2013). The three purified molecules present hydrophilic residues in their sequences especially positive charged residues, i.e., lysine and arginine.…”

In silico analysis and antihypertensive effect of ACE-inhibitory peptides from smooth-hound viscera protein hydrolysate: Enzyme-peptide interaction study using molecular docking simulation

“…was observed in several peptides such as PRGPAGPHGPP and GPAGPRGPA. In this context, Gu and Wu [41] and Lassoued et al [42] studies have shown that peptides, with the repeated motifs PX and GPX, tend to be satisfactory ACE inhibitors.…”

Peptides: Production, bioactivity, functionality, and applications

“…Finally, these chromatographic techniques are coupled to mass spectrometry (MS), in particular to tandem MS (MS/MS), for peptide sequence determination. Traditional identification approaches, which require the knowledge of the parent protein sequences, match tandem spectra with theoretical spectra derived from predicted peptides in a protein library (Espejo-Carpio et al, 2013;Gu and Wu, 2013;de Gobba et al, 2014b). Primary structure of proteins can be accessed from online databases such as UniProtKB/Swiss-Prot or NCBI and the identification process can be carried out by using database search engines (e.g., Mascot) (Dallas et al, 2015;Le-Maux et al, 2015).…”