LC–MS/MS characterization of the urinary excretion profile of the mycotoxin deoxynivalenol in human and rat

Lattanzio, Veronica M.T.; Solfrizzo, Michele; Girolamo, Annalisa De; Chulze, Sofía Noemí; Torres, Adriana Mabel; Visconti, Angelo

doi:10.1016/j.jchromb.2011.01.029

Cited by 53 publications

(34 citation statements)

References 27 publications

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“…Owing to the high concentration factor, the reported recovery was between approximately 45 and 100 %. In contrast to results obtained by various groups [15, 23, 26, 27], no DON-GlcA was detected in urine samples naturally contaminated with DON. This might indicate a loss of those conjugates during cleanup despite successful validation.…”

Section: Lc-ms/ms-based Multibiomarker Methodscontrasting

confidence: 99%

“…The first biomarker research on the trichothecene deoxynivalenol (DON, vomitoxin) was initiated in 2003 when Meky et al [13] developed an LC-MS-based assay to measure the sum of free DON and DON glucuronides (DON-GlcAs) combined after enzymatic hydrolysis and use of an immunoaffinity column (IAC) as a sum parameter in human and rat urine. Further LC-MS/MS methods were developed for the determination of DON and DON-GlcA using either a synthetically produced authentic reference standard [14] or the hypothetical mass [15] for the detection of the glucuronide(s). A major limitation of proper exposure assessment including ideally all relevant mycotoxins and their biotransformation products was the lack of sufficient sensitivity and selectivity.…”

Section: Introductionmentioning

confidence: 99%

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LC-MS/MS-based multibiomarker approaches for the assessment of human exposure to mycotoxins

2013

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Mycotoxins are toxic fungal secondary metabolites that frequently contaminate food and feed worldwide, and hence represent a major hazard for food and feed safety. To estimate human exposure arising from contaminated food, so-called biomarker approaches have been developed as a complementary biomonitoring tool besides traditional food analysis. The first methods based on radioimmunoassays and enzyme-linked immunosorbent assays as well as on liquid chromatography were developed in the late 1980s and early 1990s for the carcinogenic aflatoxins and in the last two decades further tailor-made methods for some major mycotoxins have been published. Since 2010, there has been a clear trend towards the development and application of multianalyte methods based on liquid chromatography–electrospray ionization tandem mass spectrometry for assessment of mycotoxin exposure made possible by the increased sensitivity and selectivity of modern mass spectrometry instrumentation and sophisticated sample cleanup approaches. With use of these advanced methods, traces of mycotoxins and relevant breakdown and conjugation products can be quantified simultaneously in human urine as so-called biomarkers and can be used to precisely describe the real exposure, toxicokinetics, and bioavailability of the toxins present. In this article, a short overview and comparison of published multibiomarker methods focusing on the determination of mycotoxins and relevant excretion products in human urine is presented. Special attention is paid to the main challenges when analyzing these toxic food contaminants in urine, i.e., very low analyte concentrations, appropriate sample preparation, matrix effects, and a lack of authentic, NMR-confirmed calibrants and reference materials. Finally, the progress in human exposure assessment studies facilitated by these analytical methods is described and an outlook on probable developments and possibilities is presented.FigureMycotoxin exposure assessment: traditional food analysis compared to the innovative, complementary biomarker approach

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Section: Lc-ms/ms-based Multibiomarker Methodscontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

LC-MS/MS-based multibiomarker approaches for the assessment of human exposure to mycotoxins

2013

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“…Data from that survey revealed a mean level of urinary fD þ DG about three to four times greater than the mean reported in surveys of Europeans (100) . Urinary fD þ DG has now been observed in studies from the UK, France, Sweden and China (100) , and independently in Spain and Italy (105,106) , whilst urinary DG was observed in Austrians (103) . All of the mycotoxin biomarker approaches discussed here are summarised in Table 1; ochratoxin A is additionally included for completeness, as the only other mycotoxin for which biomarker development has been reported (107) .…”

Section: Deoxynivalenolmentioning

confidence: 94%

The role of biomarkers in evaluating human health concerns from fungal contaminants in food

et al. 2012

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Mycotoxins are toxic secondary metabolites that globally contaminate an estimated 25 % of cereal crops and thus exposure is frequent in many populations. Aflatoxins, fumonisins and deoxynivalenol are amongst those mycotoxins of particular concern from a human health perspective. A number of risks to health are suggested including cancer, growth faltering, immune suppression and neural tube defects; though only the demonstrated role for aflatoxin in the aetiology of liver cancer is widely recognised. The heterogeneous distribution of mycotoxins in food restricts the usefulness of food sampling and intake estimates; instead biomarkers provide better tools for informing epidemiological investigations. Validated exposure biomarkers for aflatoxin (urinary aflatoxin M 1 , aflatoxin -N7-guaunine, serum aflatoxin -albumin) were established almost 20 years ago and were critical in confirming aflatoxins as potent liver carcinogens. Validation has included demonstration of assay robustness, intake v. biomarker level, and stability of stored samples. More recently, aflatoxin exposure biomarkers are revealing concerns of growth faltering and immune suppression; importantly, they are being used to assess the effectiveness of intervention strategies. For fumonisins and deoxynivalenol these steps of development and validation have significantly advanced in recent years. Such biomarkers should better inform epidemiological studies and thus improve our understanding of their potential risk to human health.

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“…DON urinary levels in the range 0.003-0.008 g/mL were detected in healthy human subjects, whereas DON and de-epoxynivalenol levels between 1.9-4.9 μg/mL and 1.6-5.9 μg/mL, respectively were found in rat urine. Differences in the urinary metabolite profile in human and rat have been shown (Lattanzio et al, 2011).…”

Section: Deoxynivalenolmentioning

confidence: 99%

In vivo toxicity studies of fusarium mycotoxins in the last decade: A review

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LC–MS/MS characterization of the urinary excretion profile of the mycotoxin deoxynivalenol in human and rat

Cited by 53 publications

References 27 publications

LC-MS/MS-based multibiomarker approaches for the assessment of human exposure to mycotoxins

LC-MS/MS-based multibiomarker approaches for the assessment of human exposure to mycotoxins

The role of biomarkers in evaluating human health concerns from fungal contaminants in food

In vivo toxicity studies of fusarium mycotoxins in the last decade: A review

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