LC–MS/MS-based chemometric analysis of phytochemical diversity in 13 Ficus spp. (Moraceae): Correlation to their in vitro antimicrobial and in silico quorum sensing inhibitory activities

El‐Hawary, Seham S.; Younis, Inas Y.; Bishbishy, Mahitab H. El; Khattab, Amira R.

doi:10.1016/j.indcrop.2018.10.017

Cited by 31 publications

(18 citation statements)

References 36 publications

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“…Peak 31 possessing a molecular ion peak at m / z [M − H] − 564 was allocated to apigenin‐6‐C‐pentoside‐8‐C‐hexoside, according to the data reported by Ibrahim et al (2015). Similar compounds were previously identified from the same plant by Elhawary, Younis, El Bishbishy, & Khattab (2018) and Ragab, Mohammed, Abbass, & Kotb (2013). Various apigenin C‐glycosides were previously identified in the same plant by Elhawary, Younis, El Bishbishy, & Khattab (2018).…”

Section: Resultssupporting

confidence: 81%

HPLC–ESI/MS profiling, phytoconstituent isolation and evaluation of renal function, oxidative stress and inflammation in gentamicin‐induced nephrotoxicity in rats of Ficus spragueana Mildbr. & Burret

Taher

Raslan

Masoud

et al. 2021

Biomedical Chromatography

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Ficus spragueana Mildbr. & Burret (family Moraceae) was reported to have various biological activities. However, its activity in treatment of renal injury has not been investigated yet. The current study aimed to evaluate the effects of F. spragueana leaf extract on nephrotoxicity caused by gentamicin. Gentamicin is an important broad‐spectrum antibiotic; nevertheless, it exhibits serious nephrotoxic adverse effects. HPLC–ESI/MS spectrometric analysis of the extract revealed the presence of 37 phenolic compounds. Moreover, five compounds were isolated from the leaf extract, and identified on the basis of spectroscopic analysis. The isolated compounds were syringic acid (1), p‐coumaric acid (2), 3′,5′ O‐dicaffeoylquinic acid (3), luteolin‐8‐C‐β‐D glucopyranoside (orientin) (4) and 8‐methoxy kaempferol‐3‐O‐[α‐L‐rhamnopyranosyl (1→2) β‐D‐glucopyranoside] (5). The gentamicin‐induced nephrotoxicity model was used to evaluate the protective effect of F. spragueana on renal toxicity biomarkers throughout the development of acute kidney injury. Administration of extract led to improvement in kidney function through inhibition of kidney injury molecule‐1, creatinine, blood urea nitrogen and total bilirubin, as well as decreasing the inflammatory markers interlukin1‐beta and myeloperoxidase. Furthermore, it reduced the oxidative stress by increasing reduced glutathione and total antioxidant capacity levels while decreasing malondialdehyde and nitric oxide content, and improved renal histopathological injuries.

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Section: Resultssupporting

confidence: 81%

HPLC–ESI/MS profiling, phytoconstituent isolation and evaluation of renal function, oxidative stress and inflammation in gentamicin‐induced nephrotoxicity in rats of Ficus spragueana Mildbr. & Burret

Taher

Raslan

Masoud

et al. 2021

Biomedical Chromatography

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“…The critical step in the employment of plants as medicinal with therapeutic properties is the proper separation and identi cation of bioactive secondary metabolites [19,20]. Accordingly, the bark methanol extract of Ficus religiosa (Linn.)…”

Section: Discussionmentioning

confidence: 99%

“…They have reported antioxidant and anti-tumorogenic properties viz., rutin [25], 3-caffeoylquinic acid [26], Luteolin 7-O-rutinoside [27], 6-C-glucosyl-8-C-arabinosylapigenin [28] and Kaempferol-3-O-rutinoside [29]. Potential natural quorum sensing inhibitors viz., Kaempferol-3-O-rutinoside and rutin [20] were identi ed in the current studies. However, there were still several unknown compounds present that were not identi ed in this study.…”

Section: Discussionmentioning

confidence: 99%

“…Synapt G2 (UPLC/MS separations with QuanTof) along with the Agilent zorbax SB-C18 (15 cm, 3.5 μm) column was coupled to an HCTultra ion trap MS detector was used for the qualitative analysis of the metabolites [19,20] according to manufacturers protocol. The nebulizer pressure was 60 psi at a drying temperature of 350°C and the nitrogen ow rate 10 L.min -1 was used.…”

Section: Lc Analysis Of Methanol Extract In Negative Modementioning

confidence: 99%

“…The UPLC-MS chromatogram obtained is shown in Figure 1, and peaks were labeled according to the order of their retention time. Structural characterization was performed using the retention time of standards and published data [19,20]. (+)-catechin was used as standards in this experiment.…”

Section: Lc Analysis Of Ficus Religiosa Bark Methanol Extract In Negamentioning

confidence: 99%

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Ficus religiosa (Linn.) bark extract secondary metabolites bestow antioxidant property inducing cell cytotoxicity to human breast cancer cells, MDA-MB-231 by apoptosis involving apoptosis-related proteins, Bax, Bcl-2 and PARP

Shankar

Kuppuru

Kini

et al. 2021

Preprint

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Ficus species, auspicious in many cultures worldwide are sources of novel bioactive secondary metabolites. The mechanism by which they exhibit antioxidant and apoptotic properties is scant. The main objective of the current study was to evaluate the properties of activity guided fractionated bark methanol extract of Ficus religiosa. As the methanol extract exhibited highest antioxidant activity it was evaluated for secondary metabolites and therapeutic properties. UPLC-MS analysis of the extract identified the 11 and five secondary metabolites including the rutin, 3-caffeoylquinic acid, luteolin 7-O-rutinoside, 6-C-glucosyl-8-C-arabinosylapigenin and kaempferol-3-O-rutinoside were reported. The MTT assay results identified minimal cytotoxicity for non-cancerous cell line (HEK 293 T) and maximum cell death for human breast cancer cells, MDA-MB-231 (EC50, 91.32 ± 4.21µ g.mL− 1). A high degree of DNA fragmentation in MDA-MB-231 cells subjected to the extract was observed. A clear indication of apoptosis via chromatin condensation was visualized by CLSM. The apoptotic response to treatment was also apparent in the increase in BAX along with the proteolytic cleavage of PARP-1 and a decreased Bcl-2 levels as revealed by Western blot analysis. The MDA-MB-231 cells upon exposure to the extract (91µ g.mL− 1), stimulated cells to early apoptosis (32.5%) and apoptosis (61.6%) as evidenced by flow cytometer studies. Apoptotic cells being represented by a sub G0/G1 population (86.25%) seen to the left of the G0/G1 peak were recorded. The presence of novel bioactive compounds has uncovered possible therapeutic values by modulating antioxidant and apoptosis leading to the development of potential alternative anticancer drugs.

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Chondroprotection of fruit peels in a monosodium iodoacetate‐induced osteoarthritis rat model via downregulation of Col1A1

Aborehab

Bishbishy

2022

Archiv der Pharmazie

Self Cite

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The potential of the fruit peels of mango, orange, cantaloupe, and pomegranate in the treatment of osteoarthritis (OA) was evaluated in a rat model. Their metabolic profiles were characterized using ultrahigh-performance liquid chromatography (UPLC)-electrospray ionization-mass spectroscopy and 66 albino rats were intraarticularly injected with monosodium iodoacetate in the knee joints. The extracts were orally administered at doses of 200 and 400 mg/kg for 28 days. Serum levels of IL-6 and tissue levels of cyclooxygenase-2 (COX-2), peroxisome proliferatoractivated receptor-gamma (PPARγ), and alpha-smooth muscle actin (α-SMA) were measured using ELISA. COL1A1 expression was measured by quantitative polymerase chain reaction. Histopathological changes in the joints were examined.In the extracts, 85 metabolites were annotated, and the levels of interleukin (IL)-6, COX-2, α-SMA, malondialdehyde, and nitric oxide were significantly reduced, while PPARγ and glutathione levels were significantly raised in all treated groups compared to the OA group. All extracts downregulated the cartilage mRNA expressions for COL1A1 dose-dependently. Mango peel extract exhibited the best chondroprotective effect. The in silico study showed the link between mango extract metabolites and COX-2.

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LC–MS/MS-based chemometric analysis of phytochemical diversity in 13 Ficus spp. (Moraceae): Correlation to their in vitro antimicrobial and in silico quorum sensing inhibitory activities

Cited by 31 publications

References 36 publications

HPLC–ESI/MS profiling, phytoconstituent isolation and evaluation of renal function, oxidative stress and inflammation in gentamicin‐induced nephrotoxicity in rats of Ficus spragueana Mildbr. & Burret

HPLC–ESI/MS profiling, phytoconstituent isolation and evaluation of renal function, oxidative stress and inflammation in gentamicin‐induced nephrotoxicity in rats of Ficus spragueana Mildbr. & Burret

Ficus religiosa (Linn.) bark extract secondary metabolites bestow antioxidant property inducing cell cytotoxicity to human breast cancer cells, MDA-MB-231 by apoptosis involving apoptosis-related proteins, Bax, Bcl-2 and PARP

Chondroprotection of fruit peels in a monosodium iodoacetate‐induced osteoarthritis rat model via downregulation of Col1A1

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