LC-MS metabolomics comparisons of cancer cell and macrophage responses to methotrexate and polymer-encapsulated methotrexate

Al-Natour, Mohammad Ahmad; Alazzo, Ali; Kim, Dong-Hyun; Ghaemmaghami, Amir M.

doi:10.1016/j.ijpx.2019.100036

Cited by 6 publications

(2 citation statements)

References 38 publications

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“…Further, other strategies, including the use of solid dispersion, the formation of salts or prodrugs, viscosity modifiers, or complexing, have been reported [ 6 , 8 , 9 ]. Nanotechnology has also been utilized for the entrapment of various drug types, such as the use of nanocrystals [ 10 , 11 ], solid lipid nanoparticles [ 12 ], nano-frameworks [ 13 ], PLGA nanoparticles [ 14 ], silica nanoparticles [ 15 ], chitosan nanoparticles [ 16 ], and liposomes [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Taste Masking of Promethazine Hydrochloride Using l-Arginine Polyamide-Based Nanocapsules

et al. 2023

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Promethazine hydrochloride (PMZ), a potent H1-histamine blocker widely used to prevent motion sickness, dizziness, nausea, and vomiting, has a bitter taste. In the present study, taste masked PMZ nanocapsules (NCs) were prepared using an interfacial polycondensation technique. A one-step approach was used to expedite the synthesis of NCs made from a biocompatible and biodegradable polyamide based on l -arginine. The produced NCs had an average particle size of 193.63 ± 39.1 nm and a zeta potential of −31.7 ± 1.25 mV, indicating their stability. The NCs were characterized using differential scanning calorimetric analysis and X-ray diffraction, as well as transmission electron microscopy that demonstrated the formation of the NCs and the incorporation of PMZ within the polymer. The in vitro release study of the PMZ-loaded NCs displayed a 0.91 ± 0.02% release of PMZ after 10 min using artificial saliva as the dissolution media, indicating excellent taste masked particles. The in vivo study using mice revealed that the amount of fluid consumed by the PMZ-NCs group was significantly higher than that consumed by the free PMZ group (p < 0.05). This study confirmed that NCs using polyamides based on l -arginine and interfacial polycondensation can serve as a good platform for the effective taste masking of bitter actives.

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Section: Introductionmentioning

confidence: 99%

Taste Masking of Promethazine Hydrochloride Using l-Arginine Polyamide-Based Nanocapsules

et al. 2023

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“…On the other hand, metabolomics aims for the qualitative and quantitative measurement of the end products (i.e., metabolites) of the gene expression and the cellular metabolic activities in a biological system. , The information gained using metabolomics allows the characterization of the metabolic phenotypes and therefore provides a valuable means to study the molecular effects and toxicity of polymeric NPs on the biological systems. Some studies have already been denoted for studying different NPs using metabolomics; − however, in most cases, the studies were focused on the use of metal-based NPs for cosmetic and textile applications, and few have been focused on clinical use.…”

Section: Introductionmentioning

confidence: 99%

Metabolic Signatures of Surface-Modified Poly(lactic-co-glycolic acid) Nanoparticles in Differentiated THP-1 Cells Derived with Liquid Chromatography-Mass Spectrometry-based Metabolomics

et al. 2022

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Polymeric nanoparticles (NPs) are widely used in preclinical drug delivery investigations, and some formulations are now in the clinic. However, the detailed effects of many NPs at the subcellular level have not been fully investigated. In this study, we used differentiated THP-1 macrophage cells, as a model, to investigate the metabolic changes associated with the use of poly (lactic- co -glycolic acid) (PLGA) NPs with different surface coating or conjugation chemistries. Liquid chromatography-mass spectrometry-based metabolic profiling was performed on the extracts ( n = 6) of the differentiated THP-1 cells treated with plain, Pluronic (F-127, F-68, and P-85)-coated and PEG–PLGA NPs and control (no treatment). Principal component analysis and orthogonal partial least squares-discriminant analysis (OPLS-DA) in conjunction with univariate and pathway analyses were performed to identify significantly changed metabolites and pathways related to exposure of the cells to NPs. OPLS-DA of each class in the study compared to the control showed clear separation and clustering with cross-validation values of R 2 and Q 2 > 0.5. A total of 105 metabolites and lipids were found to be significantly altered in the differentiated THP-1 cell profiles due to the NP exposure, whereas more than 20 metabolic pathways were found to be affected. These pathways included glycerophospholipid, sphingolipid, linoleic acid, arginine and proline, and alpha-linolenic acid metabolisms. PLGA NPs were found to perturb some amino acid metabolic pathways and altered membrane lipids to a different degree. The metabolic effect of the PLGA NPs on the cells were comparable to those caused by silver oxide NPs and other inorganic nanomaterials. However, PEG–PLGA NPs demonstrated a reduced impact on the cellular metabolism compared to Pluronic copolymer-coated PLGA and plain PLGA NPs.

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The toxicity of nanoparticles and their interaction with cells: an in vitro metabolomic perspective

Awashra

Młynarz

2023

Nanoscale Adv.

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LC-MS metabolomics comparisons of cancer cell and macrophage responses to methotrexate and polymer-encapsulated methotrexate

Cited by 6 publications

References 38 publications

Taste Masking of Promethazine Hydrochloride Using l-Arginine Polyamide-Based Nanocapsules

Taste Masking of Promethazine Hydrochloride Using l-Arginine Polyamide-Based Nanocapsules

Metabolic Signatures of Surface-Modified Poly(lactic-co-glycolic acid) Nanoparticles in Differentiated THP-1 Cells Derived with Liquid Chromatography-Mass Spectrometry-based Metabolomics

The toxicity of nanoparticles and their interaction with cells: an in vitro metabolomic perspective

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