LC-MS-based identification of bioactive compounds and hepatoprotective and nephroprotective activities of Bignonia binata leaves against carbon tetrachloride-induced injury in rats

Abstract

The chemical profiling of the main phytoconstituents of total ethanolic extract (TEE) and its different fractions of Bignonia binata leaves was dereplicated using liquid chromatographyhigh resolution-electrospray ionization-mass spectrometry (LC-HR-ESI-MS), revealed the presence of various classes of secondary metabolites; 8 phenylethanoids, 2 flavonoidal glycosides and 2 iridoids. Moreover, the hepatoprotective and nephroprotective activities of the TEE and its different fractions were investigated in CCl 4 -intoxicated rats and were compared with those of silymarin-treated group, revealing the highest potency of the EtOAc group, followed by the aqueous one in improving the CCl 4 -induced alterations in several biochemical parameters. Besides, EtOAc and aqueous fractions exhibited the most inhibition of CCl 4 -induced inflammatory mediators and improving the changes in the histopathological structures of the liver and kidney. Additionally, the EtOAc fraction demonstrated the highest total phenolic content, while TEE showed the highest amount of total flavonoid content.

Flavonoidal glycosides and in vitro antioxidant activity of Bignonia binata Thunb. leaves Family Bignoniaceae and in silico evidence of their potential anti-COVID-19 activity.

“…Also, in phosphomolybdate assay, the highest antioxidant capacity was obtained by the EtOAc fraction and 50%MAqF with 68.8±0.76 and 59.6±1.16 mg ascorbic acid equiv./g dried sample, respectively. Both the antioxidant activities, indicating the strong relationship between the presence of phenolics and the free radical scavenging activity, in which these samples exhibited high phenolic content and had also a potent radical scavenging activity [8]. All antioxidant results are shown in Table 3.…”

“…is distributed from Mexico to Argentina [3], Ten compounds were formerly isolated, which are belonging to phenolics, sterols, triterpenes, nitrogenous compounds and iridoids. Besides, the DPPH radical scavenging, cytotoxic, antimicrobial, antimalarial, antitrypanosomal, hepatoprotective and nephroprotective activities were previously reported [6][7][8]. Additionally, the chemical profile was investigated by LC-HR-ESI-MS, where it revealed the presence of twelve compounds related to phenylethanoids, flavonoidal glycosides and iridoids [8].…”

“…Besides, the DPPH radical scavenging, cytotoxic, antimicrobial, antimalarial, antitrypanosomal, hepatoprotective and nephroprotective activities were previously reported [6][7][8]. Additionally, the chemical profile was investigated by LC-HR-ESI-MS, where it revealed the presence of twelve compounds related to phenylethanoids, flavonoidal glycosides and iridoids [8]. Furthermore, the EtOAc fraction and total ethanolic extract of B. binata Thunb.…”

“…Furthermore, the EtOAc fraction and total ethanolic extract of B. binata Thunb. exhibited the highest amount of total phenolic and flavonoidal contents, respectively [8]. The newly emerging ailments such as the novel COVID-19 and other infectious diseases, which make serious complications on both human health and economy worldwide, need crucial research for discovering a new antiviral drug preferably from a natural source such as polyphenols [9,10].…”

Biological potential of Moluccella laevis L. aerial parts, family Lamiaceae (Labiatae)

“…The content of total phenolic content for the TEE and its derived fractions of M. laevis was determined by the Folin-Ciocalteu method [7]. Analysis was performed by adding 3.5 mL of deionized H2O, 50 μL of the TEE or its derived fractions, 50 μL Folin-Ciocalteu reagents (2N) and 300 μL of Na2CO3 (10 %).…”